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Processing technology of single-cell protein from distilled spirit lees

A technology of single-cell protein and processing technology, which is applied in food processing, animal husbandry, animal feed, etc., to achieve the effect of widening feed resources, simple process, and increasing production

Inactive Publication Date: 2014-12-03
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the new century, people have transformed from single strain fermentation to multi-bacteria mixed fermentation. However, strain selection and compatibility are only one aspect of the development of distiller's grains. Since there are no technical processes such as raw material pretreatment and solid-state fermentation medium formula, distiller's grains Development can only stay in the simple silage fermentation stage

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0019] A process for processing distiller's grain single-cell protein, characterized in that it comprises the following steps:

[0020] Step (1), distiller's grains pretreatment: air-dry the distiller's grains until the moisture content is 55%, then add 1000 units of glucoamylase per 100 g of distiller's grains, and let the distiller's grains stand for 1 hour at 50°C and pH 4.0 to obtain pre-distiller's grains. dispose of distiller's grains;

[0021] Step (2), preparation of solid-state culture medium: according to parts by weight, weigh 70 parts of pretreated distiller's grains obtained in step (1), 7 parts of wheat bran, 2 parts of corn flour, 1 part of bean cake, 0.5 part of urea, slaked lime 1 part, 0.03 part of potassium dihydrogen phosphate, and then mix them evenly to obtain a solid medium;

[0022] Step (3), inoculation and fermentation: inoculate the mixed strains into the solid medium obtained in step (2), the inoculum amount is 5%; then after 48 hours of fermentati...

Embodiment 2

[0026] A process for processing distiller's grain single-cell protein, characterized in that it comprises the following steps:

[0027] Step (1), distiller's grains pretreatment: air-dry the distiller's grains until the water content is 65%, then add 1000 units of glucoamylase per 100 g of distiller's grains, and let the distiller's grains stand for 2 hours at 60°C and pH 4.2 to obtain pre-distiller's grains dispose of distiller's grains;

[0028] Step (2), preparation of solid-state medium: according to parts by weight, weigh 90 parts of pretreated distiller's grains obtained in step (1), 20 parts of wheat bran, 5 parts of corn flour, 5 parts of bean cake, 3 parts of urea, and slaked lime 3 parts, 0.06 parts of potassium dihydrogen phosphate, and then mix them evenly to obtain a solid medium;

[0029] Step (3), inoculation and fermentation: inoculate the mixed strains into the solid medium obtained in step (2), the inoculum amount is 5%; then after 48 hours of fermentation, ...

Embodiment 3

[0033] A process for processing distiller's grain single-cell protein, characterized in that it comprises the following steps:

[0034] Step (1), distiller's grains pretreatment: air-dry distiller's grains until the water content is 60%, then add 1000 units of glucoamylase per 100 g of distiller's grains, and let distiller's grains stand at 55°C and pH 4.1 for 1.4 hours to obtain pre-distiller's grains dispose of distiller's grains;

[0035] Step (2), preparation of solid medium: according to parts by weight, weigh 85 parts of pretreated distiller's grains obtained in step (1), 13 parts of wheat bran, 3 parts of corn flour, 4 parts of bean cake, 2 parts of urea, and slaked lime 2 parts, 0.05 parts of potassium dihydrogen phosphate, and then mix them evenly to obtain a solid medium;

[0036] Step (3), inoculation and fermentation: inoculate the mixed strains into the solid medium obtained in step (2), the inoculum amount is 5%; then after 48 hours of fermentation, dry at 65°C ...

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PUM

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Abstract

The invention discloses a processing technology of a single-cell protein from distilled spirit lees and relates to the technical field of animal nutrition and microbial fermentation. A finished product is obtained by performing pretreatment on the distilled spirit lees, then reasonably combining the lees with various ingredients according to a formula of a solid-state culture medium, fully mixing, then inoculating, fermenting for 48h, then drying and finally packaging. The processing technology comprises the steps of pretreatment of the lees, preparation of the solid-state culture medium, inoculation, fermentation, drying, packaging and obtainment of the finished product. The pretreatment comprises the steps that the distilled spirit lees is aired till the water content is 55-65%, then 600-1000 units of glucoamylase is added in per 100g of the distilled spirit lees, and the pretreatment of the lees is performed for 1-2h under the conditions that the temperature is 50-60 DEG C and the pH is 4.0-5.2. The processing technology disclosed by the invention can develop the distilled spirit lees into a single-cell protein fodder by combining with a microbial fermentation technology on the basis of animal nutrition theory, thereby turning waste into treasure and exploring fodder resources; simultaneously, the processing technology disclosed by the invention has the characteristics of simple technology, low cost and no pollution.

Description

technical field [0001] The invention belongs to the technical field of animal nutrition and microbial fermentation, and in particular relates to a processing technology of distiller's grain single-cell protein. Background technique [0002] my country is a big wine producing country, and distiller's grain waste resources are very rich. According to statistics, about 3×10 7 More than tons of distiller's grains. Distiller's grains have high water content, high acidity, and are perishable and deteriorated, resulting in serious waste of distiller's grains, and a considerable part of distiller's grains contains about 30% of indigestible chaff, resulting in high crude fiber content of distiller's grains, poor palatability, and low digestibility. Seriously affect the feeding value and feeding effect of distiller's grains. [0003] Many domestic scholars have done relevant research on the development of distiller's grains as a protein feed. As early as the 1980s, scholars had stu...

Claims

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Application Information

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IPC IPC(8): A23K1/06A23K1/00A23K10/12A23K10/14A23K10/38
CPCY02P60/87
Inventor 薛白王之盛焦浩鹏王立志
Owner SICHUAN AGRI UNIV
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