A method for preparing Enterococcus faecalis by solid-state fermentation

A technology of Enterococcus faecalis and solid-state fermentation, which is applied in the field of preparation of lactic acid bacteria, can solve the problems of high cost, low protein content, poor comprehensive performance and the like, and achieves the effects of low product cost, high protein content and high number of viable bacteria.

Active Publication Date: 2019-09-17
上海邦成生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Enterococcus faecalis product produced by the invention has good preservation and high bacteria content, but the cost is high, the protein content is low, and the comprehensive performance is poor

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for preparing Enterococcus faecalis by solid-state fermentation, the method comprising the following steps:

[0027] (1) Use the MRS medium as the seed medium, cultivate Enterococcus faecalis for 20 hours at 35° C. under the condition of a rotating speed of 180 rpm to obtain a first-level seed liquid; the first-level seed medium includes the following components by weight percentage: Ingredients: peptone 10g / L, yeast extract 15g / L, anhydrous sodium acetate 5g / L, dipotassium hydrogen phosphate 2g / L, ammonium citrate 2g / L, magnesium sulfate 0.58g / L, manganese sulfate 0.25g / L, Glucose 20g / L, pH7.0±0.2;

[0028] (2) Inoculate the primary seed solution into the seed tank for expanded cultivation, the MRS medium is the seed medium, and under the microaerobic condition of 35°C (the oxygen content is 0.15m 3 / min) cultured for 20h to obtain the secondary seed liquid; the secondary seed medium includes the following components in weight percentage: peptone 10g / L, yeast...

Embodiment 2

[0033] A method for preparing Enterococcus faecalis by solid-state fermentation, the method comprising the following steps:

[0034] (1) Use the MRS medium as the seed medium, cultivate Enterococcus faecalis for 18 hours under microaerobic conditions at 37° C. (180 rpm / min), to obtain a primary seed liquid; the primary seed medium includes the following weight percentages Components: peptone 10g / L, yeast extract 15g / L, anhydrous sodium acetate 5g / L, dipotassium hydrogen phosphate 2g / L, ammonium citrate 2g / L, magnesium sulfate 0.58g / L, manganese sulfate 0.25g / L L, glucose 20g / L, pH7.0±0.2;

[0035] (2) Inoculate the first-level seed solution into the seed tank for expanded cultivation, MRS medium is the seed medium, and under the microaerobic condition of 37°C (oxygen content is 0.15m 3 / min) for 18 hours to obtain secondary seed liquid; the secondary seed medium includes the following components by weight percentage: peptone 10g / L, yeast extract 15g / L, anhydrous sodium acetat...

Embodiment 3

[0040] A method for preparing Enterococcus faecalis by solid-state fermentation, the method comprising the following steps:

[0041] (1) Use the MRS medium as the seed medium, and cultivate Enterococcus faecalis for 16 hours under microaerobic conditions at 40°C (180 rpm / min) to obtain a primary seed liquid; the primary seed medium includes the following weight percentages Components: peptone 10g / L, yeast extract 15g / L, anhydrous sodium acetate 5g / L, dipotassium hydrogen phosphate 2g / L, ammonium citrate 2g / L, magnesium sulfate 0.58g / L, manganese sulfate 0.25g / L L, glucose 20g / L, pH7.0±0.2;

[0042] (2) Inoculate the first-level seed solution into the seed tank for expanded cultivation, MRS medium is the seed medium, and under the microaerobic condition of 40°C (oxygen content is 0.15m 3 / min) cultured for 16 hours to obtain the secondary seed liquid; the secondary seed medium includes the following components in weight percentage: peptone 10g / L, yeast extract 15g / L, anhydrous s...

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PUM

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Abstract

The invention relates to a method using solid-state fermentation to prepare enterococcus faecalis. The method includes the steps of firstly, inoculating enterococcus faecalis seeds into a primary seed culture medium, and culturing at 35-40 DEG C under a micro-aerobic condition for 16-25 hours to obtain a primary seed solution; secondly, inoculating the primary seed solution obtained in the first step into a secondary seed culture medium of a seed tank, and performing amplification culture at 35-40 DEG C under a micro-aerobic condition for 16-20 hours to obtain a secondary seed solution; thirdly, inoculating the secondary seed solution obtained in the second step into the fermentation culture medium of a fermentation tank for culture, and fermenting at 35-40 DEG C under a micro-aerobic condition for 16-30 hours to obtain a tertiary seed solution; fourthly, evenly mixing the tertiary seed solution obtained in the third step with a solid-state culture medium, bagging, and performing fermentation culture for 36-80 hours to obtain a fresh fermentation material; fifthly, drying the fresh fermentation material obtained in the fourth step to obtain the enterococcus faecalis prepared by solid-state fermentation. Compared with the prior art, the method is simple in preparation process, low in production cost, high in viable count of the enterococcus faecalis, and the like.

Description

technical field [0001] The invention relates to a preparation method of lactic acid bacteria, in particular to a method for preparing Enterococcus faecalis by solid-state fermentation. Background technique [0002] Enterococcus faecalis is a class of lactic acid bacteria belonging to the genus Enterococcus of the family Streptococcaceae. Spherical cells, arranged in chains, non-spore-forming, facultative anaerobic, Gram-positive cocci. Enterococcus faecalis can ferment a variety of sugars, producing acid without producing gas, which belongs to the same type of fermentation. Enterococcus faecalis can grow at 10°C and 45°C, has high chemical resistance, can grow in the environment of 6.5% NaCl and pH 9.6, etc., and is widely distributed in nature. Enterococcus faecalis is a normal flora in the intestines of humans and animals, and is also an important lactic acid bacteria, which has good intestinal adhesion ability, can produce lactic acid and some antibacterial substances, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01
Inventor 肖前青郑云峰陆克文
Owner 上海邦成生物工程有限公司
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