A method for preparing Enterococcus faecalis by solid-state fermentation
A technology of Enterococcus faecalis and solid-state fermentation, which is applied in the field of preparation of lactic acid bacteria, can solve the problems of high cost, low protein content, poor comprehensive performance and the like, and achieves the effects of low product cost, high protein content and high number of viable bacteria.
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Embodiment 1
[0026] A method for preparing Enterococcus faecalis by solid-state fermentation, the method comprising the following steps:
[0027] (1) Use the MRS medium as the seed medium, cultivate Enterococcus faecalis for 20 hours at 35° C. under the condition of a rotating speed of 180 rpm to obtain a first-level seed liquid; the first-level seed medium includes the following components by weight percentage: Ingredients: peptone 10g / L, yeast extract 15g / L, anhydrous sodium acetate 5g / L, dipotassium hydrogen phosphate 2g / L, ammonium citrate 2g / L, magnesium sulfate 0.58g / L, manganese sulfate 0.25g / L, Glucose 20g / L, pH7.0±0.2;
[0028] (2) Inoculate the primary seed solution into the seed tank for expanded cultivation, the MRS medium is the seed medium, and under the microaerobic condition of 35°C (the oxygen content is 0.15m 3 / min) cultured for 20h to obtain the secondary seed liquid; the secondary seed medium includes the following components in weight percentage: peptone 10g / L, yeast...
Embodiment 2
[0033] A method for preparing Enterococcus faecalis by solid-state fermentation, the method comprising the following steps:
[0034] (1) Use the MRS medium as the seed medium, cultivate Enterococcus faecalis for 18 hours under microaerobic conditions at 37° C. (180 rpm / min), to obtain a primary seed liquid; the primary seed medium includes the following weight percentages Components: peptone 10g / L, yeast extract 15g / L, anhydrous sodium acetate 5g / L, dipotassium hydrogen phosphate 2g / L, ammonium citrate 2g / L, magnesium sulfate 0.58g / L, manganese sulfate 0.25g / L L, glucose 20g / L, pH7.0±0.2;
[0035] (2) Inoculate the first-level seed solution into the seed tank for expanded cultivation, MRS medium is the seed medium, and under the microaerobic condition of 37°C (oxygen content is 0.15m 3 / min) for 18 hours to obtain secondary seed liquid; the secondary seed medium includes the following components by weight percentage: peptone 10g / L, yeast extract 15g / L, anhydrous sodium acetat...
Embodiment 3
[0040] A method for preparing Enterococcus faecalis by solid-state fermentation, the method comprising the following steps:
[0041] (1) Use the MRS medium as the seed medium, and cultivate Enterococcus faecalis for 16 hours under microaerobic conditions at 40°C (180 rpm / min) to obtain a primary seed liquid; the primary seed medium includes the following weight percentages Components: peptone 10g / L, yeast extract 15g / L, anhydrous sodium acetate 5g / L, dipotassium hydrogen phosphate 2g / L, ammonium citrate 2g / L, magnesium sulfate 0.58g / L, manganese sulfate 0.25g / L L, glucose 20g / L, pH7.0±0.2;
[0042] (2) Inoculate the first-level seed solution into the seed tank for expanded cultivation, MRS medium is the seed medium, and under the microaerobic condition of 40°C (oxygen content is 0.15m 3 / min) cultured for 16 hours to obtain the secondary seed liquid; the secondary seed medium includes the following components in weight percentage: peptone 10g / L, yeast extract 15g / L, anhydrous s...
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