Method for processing vancomycin fermentation waste slag with solid-state fermentation

A technology for solid-state fermentation and fermentation waste residue, which is used in the removal of solid waste, fungi, etc.

Inactive Publication Date: 2008-08-20
SHANGHAI LAIYI BIOMEDICAL RES & DEV CENT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the defects in the existing vancomycin fermentation waste residue treatm

Method used

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  • Method for processing vancomycin fermentation waste slag with solid-state fermentation
  • Method for processing vancomycin fermentation waste slag with solid-state fermentation
  • Method for processing vancomycin fermentation waste slag with solid-state fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 , the acquisition of yeast seed liquid

[0019] Prepare the seed medium according to the following formula:

[0020] Malt extract powder 8.0%; Yeast powder 0.1%; K 2 HPO 4 0.1%; urea 0.1%; the rest is water; pH6.0.

[0021] After the culture medium was subpackaged and sterilized, various yeasts were added as shown in Table 1, and cultured with shaking at 30°C for 24 hours to obtain yeast seed liquid.

[0022] Table 1

[0023]

Embodiment 2～12

[0024] Embodiment 2~12 , Solid-state fermentation treatment of vancomycin fermentation waste residue

[0025] Take the vancomycin fermentation waste residue, prepare it into a solid medium with an initial water content of ≤80%, and then put it in an oven at 100°C for about 5 minutes for disinfection; then insert the yeast seed solution according to the inoculation amount of about 10% , under the condition of 20-40° C., and cultured statically for 10 hours to 7 days, see Table 2 for details. (Note: Although the conditions and inoculation amount of the above disinfection treatment have been clearly defined, for those skilled in the art, it can be determined according to the actual situation, such as the amount of waste residue, the initial water content of the culture medium and the bacteria in the seed solution. Appropriate adjustments for concentration, etc., are obvious).

[0026] Among them, turn once 12 hours after inoculation, turn again after 24 hours, and turn once ev...

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Abstract

The invention provides a processing method of vancomycin fermentation residue. The invention makes use of vancomycin fermentation residue as a sole culture medium, and the solid state culture method is adopted under the proper conditions for culturing yeast. The obtained yeast culture dry powder does not contain vancomycin residue after the culture and proper drying and smashing. Thus, the method not only solves the vancomycin residue problem in vancomycin fermentation residue and realizes the harm-free processing, but also can effectively cultures yeast and obtains a great deal of yeast protein with application value.

Description

technical field [0001] The invention belongs to the fields of fermentation waste residue treatment and yeast cultivation, and in particular relates to a method for treating vancomycin fermentation waste residue through yeast solid-state fermentation. Background technique [0002] Vancomycin, a glycopeptide antibiotic, is currently the first choice clinically for the treatment of severe infections caused by methicillin-resistant Staphylococcus aureus, and most of it is produced by microbial fermentation and culture. [0003] After the fermentation of vancomycin is completed, the fermented liquid is pretreated to a certain extent, and the useful clear liquid is filtered out. In the remaining fermentation waste residue, the main components are the mycelium of the vancomycin-producing bacteria and the unmetabolized substance in the original culture medium. Utilized organic matter, inorganic salts and a small amount of vancomycin, etc.; it is characterized by being rich in organi...

Claims

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Application Information

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IPC IPC(8): C12N1/16B09B3/00
Inventor 陈代杰金一平戈梅罗敏玉张建斌阮林高叶伟东吴亚铭阮丽军左明丽
Owner SHANGHAI LAIYI BIOMEDICAL RES & DEV CENT
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