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Process for solid culture of hair weeds cells

A technology of cell culture and solid-state culture, applied in the field of cell culture, to achieve the effects of simple method, strong drought resistance, high economic value and environmental protection value

Inactive Publication Date: 2006-06-28
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention is a research aimed at the above-mentioned problems, and its purpose is to provide a method that is simple and easy to operate, which is beneficial to the protection and artificial production of the endangered species of Nostocchia, in the hope that the artificial cultivation of Nostocchi algae is progressing slowly at present. Under the circumstances, realize complete artificial cultivation, and can expand production under close to natural conditions or directly inoculated on sand or desert soil natural sand surface, so that it can grow relatively fast solid-state culture method of Nostocella cells

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: The solid-state culture of Nostoc sativa cells in a sand table, the specific method is:

[0016] A. Preparation of culture medium: NaNO 3 0.5g / L, MgSO 4 ·7H 2 O 75mg / L, CaCl 2 2H 2 O 30mg / L, NaSiO 3 9H 2 O55mg / L, K 2 HPO 4 35mg / L, pH7.5, mix well and set aside.

[0017] B. Preparation of Nostocchia cell liquid: Centrifuge or filter the Nostocchia cell culture solution, and collect Nostocyst cells. According to the ratio of 10g (wet weight) of Nostocchia cells to 1L of culture solution, resuspend them, or use dry Nostocchi cells, soak them for 24 hours at the ratio of 2.5g of cell powder to 1L of culture solution, to restore their activity, and use them as solid-state culture Use species.

[0018] C. Sand pretreatment: sieve the sand through a 40-mesh sieve, wash with water, and dry at 100°C until constant weight. Spread flat on glass, plastic, metal or wooden platters, 0.5cm thick.

[0019] D. Cell culture: the cell liquid of Nostocchis 400ml...

Embodiment 2

[0026] A. Preparation of culture medium: NaNO 3 1.0g / L, MgSO 4 ·7H 2 O 75mg / L, CaCl 2 2H 2 O 35mg / L, NaSiO 3 9H 2 O60mg / L, K 2 HPO 4 37.5mg / L, pH7.25 (pH7.8), mix well and set aside.

[0027] C. Sand pretreatment: sieve the sand through a 40-mesh sieve, wash with water, and dry at 100°C until constant weight. Spread flat on glass, plastic, metal or wooden platters, 1.0cm thick.

[0028] D. Cell culture: the cell liquid of Nostocchis 600ml / m 2 The amount of inoculum is inoculated on the sand surface, according to 1.2L / m 2 The total consumption is divided into 3 times and spray culture solution or water every day during the light period, and the sand tray is covered with a transparent plastic film to keep the surface of the sand grains moist.

[0029] E. Culture conditions: temperature 25°C, light 120μmol m -2 the s -1 , Light and dark cycle 12h:12h.

[0030] All the other are with embodiment 1.

Embodiment 3

[0032] A. Preparation of culture medium: NaNO 3 1.5g / L, MgSO 4 ·7H 2 O 75mg / L, CaCl 2 2H 2 O 40mg / L, NaSiO 3 9H 2 O65mg / L, K 2 HPO4 40mg / L, pH8.0, mix well and set aside.

[0033] C. Sand pretreatment: sieve the sand through a 40-mesh sieve, wash with water, and dry at 100°C until constant weight. Spread flat on glass, plastic, metal or wooden platters, 1.5cm thick.

[0034] D. Cell culture: the cell liquid of Nostocella spp. is 800ml / m 2The amount of inoculum is inoculated on the sand surface, according to 1.5L / m 2 The total consumption is divided into 3 times and spray culture solution or water every day during the light period, and the sand tray is covered with a transparent plastic film to keep the surface of the sand grains moist.

[0035] E. Culture conditions: temperature 26°C, light 180μmol·m -2 the s -1 , light and dark cycle 12h:12h;

[0036] All the other are with embodiment 1.

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Abstract

The invention discloses fa-tsai cell solid state culturing method. It solves the problem of relative fast growth. Its technical proposal is as follows: inoculating fa-tsai cell suspending liquid on solid state culture ground substance surface; spraying culture solution; culturing at manual control or natural condition; processing dry-wet rhythm culture; realizing full manual control or field enlarging culture. The technique increases breeding speed. The formed product has strong drought resistance and can preserve for long time; it can purify air and not produce poisoning matter; its enlarging culture can maintain ecological balance and reform desertification soil; thus it has high economic and environmental protection value.

Description

technical field [0001] The invention relates to a cell culture technology, in particular to a cell culture technology of Nostocs edulis, in particular to a solid-state culture method of cells of Nostocs edulis which can be directly inoculated on sand grains or the natural sandy soil surface of desert soil. Background technique [0002] Nostoc flagelliforme is a prokaryotic organism belonging to Procaryotae, Cyanophyta, Cyanophyceae, Hormogonales, Nostocaceae, and Nostoc (Nostoc). It is rich in nutrition, has certain medicinal value, has great economic development value and potential, and has long been favored by people because its pronunciation is similar to "facai". Nostoc is mainly distributed in the desert and semi-desert areas of Northwest China and some provinces in North China. Nostoc can not only perform photosynthesis, but also fix nitrogen. It is a terrestrial cyanobacteria that lives under special ecological conditions. It is resistan...

Claims

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Application Information

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IPC IPC(8): C12N1/12A01G33/00
Inventor 贾士儒苏建宇何茜陈雪峰于海峰
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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