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Bt solid bottom fermentation and continuous transplantation production method

A technology of thuringiensis spores and submerged fermentation, applied in the direction of bacteria, etc., can solve the problem of material thickness, air purification and air supply volume, temperature and humidity control that cannot meet the standardization and standardization requirements, geographical, climate, environmental influence and restrictions, production temperature Humidity is difficult to control and other problems, to achieve the effect of reducing the possibility of bacteriophage contamination, not easy to infect bacteria, and stable production process

Inactive Publication Date: 2004-11-17
GUANGDONG BEIDA NEW CENTURY BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, the solid-state fermentation of Bacillus thuringiensis mostly adopts the shallow plate type, warehouse type shallow plate type, box type, and barrel type in various forms. Air volume, temperature and humidity control cannot meet the standardization and standardization requirements, and production is often affected and restricted by region, climate, and environment. Especially in summer, it is difficult to control the temperature and humidity of production. Some medium components are not easy to crush during the crushing process, resulting in high energy consumption and waste of production. Large, unstable product quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] Bacillus thuringiensis solid-state submerged fermentation process: liquid-state amplified culture of strains, medium sterilization and inoculation, solid-state submerged fermentation, drying, and crushing.

[0009] 1. Fermenter medium formula and preparation method: 665 kg of wheat bran, 14 kg of fish meal, 14 kg of yeast powder, mix the medium materials together and mix well, put 7 kg of lime into 735 kg of water, and take the supernatant Stir well with the medium mixture.

[0010] 2. Sterilization of the culture medium: put the culture medium in a sterilizer for high temperature sterilization at 121°C for 35 minutes.

[0011] 3. Inoculation and seed dressing: Inoculation of strains in sand tubes, breeding in eggplant bottles, liquid cultivation in seed tanks, inoculation of medium at room temperature and mixing in the kettle, and then distribution of materials.

[0012] 4. Solid state submerged fermentation:

[0013] 4.1 The fermenter is set in the fermentation room...

Embodiment 2

[0018] Bacillus thuringiensis solid-state submerged fermentation process: the culture medium is sterilized, the solid-state submerged fermentation product is used as the strain to transfer and then fermented, dried and crushed.

[0019] 1. Fermenter medium formula and preparation method: according to the proportion of the medium formula in Example 1, wheat bran, fish meal, yeast powder, and lime are each multiplied by 5 times, totaling 3500 kg, and the medium materials are mixed together and mixed well. Put 35 kilograms of lime into 3675 kilograms of water, take its supernatant and mix with the culture medium and mix well.

[0020] 2. Sterilization of the culture medium: put the culture medium in a sterilizer for high temperature sterilization at 121°C for 35 minutes.

[0021] 3. The solid-state fermented product is used as a strain for transfer and then fermented:

[0022] 3.1 Each fermenter in the fermentation room is covered with a layer of sterile wet cloth for air filtra...

Embodiment 3

[0026] Bacillus thuringiensis solid-state deep-layer continuous transfer and re-fermentation: medium sterilization, solid-state bacteria continuous transfer and re-fermentation, drying, crushing.

[0027] 1. Culture medium formula and preparation method: culture medium formula, ratio, quantity and preparation method are with embodiment two (1);

[0028] 2. Sterilization of the culture medium: put the culture medium in a sterilizer for high temperature sterilization at 121°C for 35 minutes.

[0029] 3. Continuous transfer and re-fermentation of solid-state strains:

[0030] 3.1 After the medium is sterilized, it is sent to the fermenter, and it is paved by a turning machine with a thickness of 800mm.

[0031] 3.2 Then extract the fermented product of Example 2 (3.2) which has been fermented to the number of logarithmic bacteria at 20 billion / mg with a material transfer machine, and transfer it to 10 fermenters as strains, and then carry out re-fermentation after uniform seed d...

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Abstract

The invention discloses a Bacillus thuringiensis solid bottom fermentation and continuous transplantation production method which comprises, (1) mixing the liquid state bacterial with solid state culture medium, storing in fermentation apparatus for fermentation, (2) treating the fermentate as bacterial to carry out continuous transfer and second fermentation. The invention realizes increased production efficiency.

Description

technical field [0001] The invention relates to a microbial solid-state fermentation method, in particular to a production method of Bacillus thuringiensis solid-state submerged fermentation and continuous transfer. Background technique [0002] At present, the solid-state fermentation of Bacillus thuringiensis mostly adopts shallow plate type, warehouse type shallow plate type, box type, and barrel type in different forms. Air volume, temperature and humidity control cannot meet the standardization and standardization requirements. Production is often affected and restricted by region, climate, and environment. Especially in summer, it is difficult to control the temperature and humidity of production. Some medium components are not easy to crush during the crushing process, resulting in high energy consumption and waste of production. Large, unstable product quality. Contents of the invention [0003] The object of the present invention is to provide a production method...

Claims

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Application Information

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IPC IPC(8): C12N1/20
Inventor 田锋张世坚王胜华黄国勇
Owner GUANGDONG BEIDA NEW CENTURY BIOENG
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