Tetramethylpyrazine bacillus amyloliquefaciens, and applications of sesame-flavor baijiu special-purpose mouldy bran

A technology for tetramethylpyrazine sesame fragrance and starch spore solution, which is applied in the field of brewing engineering, can solve problems such as the production of sesame fragrance bran koji, etc., and achieve the effects of increasing fullness and layering, product stability, and clear functions

Inactive Publication Date: 2017-07-28
ANHUI XUANJIU GRP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese Patent Authorization Announcement No. CN102766592B, titled Bacillus amyloliquefaciens and its uses, discloses a strain of bacteria whose preservation number is CGMCC No.5740 Bacillus amyloliquefaciens JNC-002, which has the ability to produce tetramethylpyrazine, But so far there is no report that it is used in the production of sesame fragrant bran koji

Method used

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  • Tetramethylpyrazine bacillus amyloliquefaciens, and applications of sesame-flavor baijiu special-purpose mouldy bran
  • Tetramethylpyrazine bacillus amyloliquefaciens, and applications of sesame-flavor baijiu special-purpose mouldy bran

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Embodiment 1

[0023] In the present embodiment, the preparation method of special bran yeast for tetramethylpyrazine sesame-flavored liquor is as follows:

[0024] 1. Preparation of the primary seed liquid of Bacillus amyloliquefaciens

[0025] Use an inoculation loop to pick colonies from the pure culture slant of the Bacillus amyloliquefaciens XJB-104 strain with the strain preservation number CCTCC M 2017133 and place them in the primary seed medium at 37°C, 160rpm, After 24 hours, the primary seed solution was obtained; the primary seed medium: peptone 10g / L, yeast extract powder 5g / L, NaCl 15g / L;

[0026] 2. Preparation of secondary seed liquid of Bacillus amyloliquefaciens

[0027] Inoculate the above-mentioned first-level seed liquid into the second-level seed medium with an inoculum amount of 1% (V / V), cultivate it for 20-25h at 37°C and 160rpm, and obtain the second-level seed liquid; wherein the second-level seed medium: glucose 70g / L, peptone 30g / L, yeast extract 10g / L, diammo...

Embodiment 2

[0034] In the present embodiment, the preparation method of special bran yeast for tetramethylpyrazine sesame-flavored liquor is as follows:

[0035] 1. Preparation of the primary seed liquid of Bacillus amyloliquefaciens

[0036] Use an inoculation loop to pick colonies from the pure culture slant of the Bacillus amyloliquefaciens XJB-104 strain with the strain preservation number CCTCC M 2017133 and place them in the primary seed medium at 37°C, 160rpm, After 24 hours, the primary seed solution was obtained; the primary seed medium: peptone 10g / L, yeast extract powder 5g / L, NaCl 15g / L;

[0037] 2. Preparation of secondary seed liquid of Bacillus amyloliquefaciens

[0038] Inoculate the above-mentioned first-level seed liquid into the second-level seed medium with an inoculum amount of 1% (V / V), cultivate it for 20-25h at 37°C and 160rpm, and obtain the second-level seed liquid; wherein the second-level seed medium: glucose 70g / L, peptone 30g / L, yeast extract 10g / L, diammo...

Embodiment 3

[0044] In the present embodiment, the preparation method of special bran yeast for tetramethylpyrazine sesame-flavored liquor is as follows:

[0045] 1. Preparation of the primary seed liquid of Bacillus amyloliquefaciens

[0046] Use an inoculation loop to pick colonies from the pure culture slant of the Bacillus amyloliquefaciens XJB-104 strain with the strain preservation number CCTCC M 2017133 and place them in the primary seed medium at 37°C, 160rpm, After 24 hours, the primary seed solution was obtained; the primary seed medium: peptone 10g / L, yeast extract powder 5g / L, NaCl 15g / L;

[0047] 2. Preparation of secondary seed liquid of Bacillus amyloliquefaciens

[0048] Inoculate the above-mentioned first-level seed liquid into the second-level seed medium with an inoculation amount of 1% (V / V), cultivate it for 20-25h at 37°C and 160rpm, and obtain the second-level seed liquid; wherein the second-level seed medium: glucose 70g / L, peptone 30g / L, yeast extract 10g / L, dia...

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Abstract

The invention discloses a tetramethylpyrazine bacillus amyloliquefaciens, and applications of a sesame-flavor baijiu special-purpose mouldy bran. The tetramethylpyrazine sesame-flavor baijiu special-purpose mouldy bran is obtained using tetramethylpyrazine bacillus amyloliquefaciens XJB-104 strain via following steps: preparation of a primary seed solution, preparation of a secondary seed solution, bran solid state culture medium fermentation, and aeration-drying of an obtained matured mouldy bran material. The obtained tetramethylpyrazine sesame-flavor baijiu special-purpose mouldy bran is bright in color, is dark brown, possesses typical smell, and slight ammonia smell, and is beneficial for improvement of the quality of sesame-flavor baijiu; and the tetramethylpyrazine content is higher than 380mg/kg.

Description

technical field [0001] The invention belongs to the technical field of brewing engineering, and in particular relates to tetramethylpyrazine bacillus amyloliquefaciens and its use in preparing sesame-flavored liquor special-purpose bran koji. Background technique [0002] According to the "Pharmacopoeia", tetramethylpyrazine, also known as tetracycline, is a heterocyclic, nitrogen-containing colorless crystal compound, which was first isolated and extracted from the rhizome of the Chinese herbal medicine Chuanxiong. Conventional medicines for diseases. According to China's "China Brewing" (2007 Issue 10, pages 60-61) and "Brewing Technology" (2012 Issue 3, pages 17-22), the content of tetramethylpyrazine in sesame-flavored liquor is relatively high , it not only makes an important contribution to the flavor of sesame-flavored liquor, but also endows sesame-flavored liquor with health benefits. Increasing the content of tetramethylpyrazine in sesame-flavored liquor is benefi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12G3/02C12R1/07
CPCC12G3/02C12N1/205C12R2001/07
Inventor 周萍叶明张温清司冠儒梅婕汪家胜郭志忠
Owner ANHUI XUANJIU GRP
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