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Biosynthesis method of perylenequinonoid compounds

A biosynthesis and compound technology, which is applied in the field of biosynthesis of perylenequinone compounds, can solve the problems of serious environmental pollution, complex production process, and low production efficiency, and achieve the effects of overcoming many processes, ensuring product quality, and improving production efficiency

Inactive Publication Date: 2016-06-01
赵建英
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  • Application Information

AI Technical Summary

Problems solved by technology

In the current biosynthesis method of perylenequinone compounds, the cost of the finished product is too high, the environmental pollution is serious, the production process is complicated, the production efficiency is low, and the storage time is short

Method used

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Embodiment

[0011] The biosynthesis method of the perylenequinone compound of the present embodiment comprises the following steps:

[0012] (1) The fungi that grow in the mountainous area of ​​western Yunnan and are identified as Ascomycetes, Hypocreataceae, and Mycoparasites are used as the strains, and the solid mixture containing corn flour, wheat bran and bean dregs is used as the solid medium;

[0013] (2) After sterilizing the solid-state medium, insert the strain, ferment at 23°C for 9 days, and obtain a brick-red solid fermented product containing perylenequinone compounds;

[0014] (3) The brick-red solid fermented product is dried and pulverized, and then extracted and separated to obtain the perylenequinone compound product.

[0015] The biosynthesis method of the perylene quinone compound can directly produce the perylene quinone compound without further processing, the process is simple, and the produced product has high yield, good quality and long storage time.

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Abstract

The invention discloses a biosynthesis method of perylenequinonoid compounds, in particular a biosynthesis method of perylenequinonoid compounds and specifically relates to optimization of a fermentation process in a biosynthesis process. A fungus, which grows in Yunnan western mountainous area and is identified to belong to ascomycetes, hypocreaceae and hypornyces, serves as a strain and a solid mixture, which contains maize powder, wheat bran and bean drges, is taken as a solid-state culture medium. The solid-state culture medium is sterilized and the strain is inoculated, and fermentation is conducted at 21-23 DEG C for no less than 9 days, so that a brick-red solid fermented product containing the perylenequinonoid compounds is obtained, therefore a bacterium block containing the perylenequinonoid is obtained through biosynthesis, wherein the bacterium block contains biosynthesis products of the invention, namely such perylenequinonoid compounds as elsinochrome A, hypocrellin A, hypocrellin B, hypomycin A and B, and the like. A finished perylenequinonoid compound product can be obtained by drying and crushing the bacterium block and by extracting and separating. According to the biosynthesis method disclosed by the invention, the total amount of the perylenequinonoid compounds in the finished product is improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a biosynthesis method of a perylenequinone compound. Background technique [0002] Perylenequinone derivatives are a class of compounds with 4,9-dihydroxy-3,10 perylenequinone as the active center, and they are widely distributed in animals and fungi. Perylenequinone derivatives as photosensitizers are one of the frontiers and hotspots of photosensitization research in recent years. In the current biosynthesis method of perylenequinone compounds, the cost of the finished product is too high, the environment is seriously polluted, the production process is complicated, the production efficiency is low, and the storage time is short. Contents of the invention [0003] In order to overcome the above-mentioned technical problems existing in the prior art field, the object of the present invention is to provide a biosynthesis method of perylenequinone compound. The pres...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P15/00C12R1/645
Inventor 赵建英
Owner 赵建英
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