58 results about "Biosynthetic process" patented technology

The invention discloses a bacterial cellulose membrane in a gradient structure and a preparation method of the bacterial cellulose membrane. The bacterial cellulose membrane is prepared by the steps of adding crop starch in a fermentation culture solution, heating for gelatinizing the mixture, adding a thickening agent into the gelatinized mixture, and carrying out static culture; and the bacterial cellulose membrane has a gradient structure with continuous transition from a compact upper surface downwards to a loose lower surface. The in-site fast controllable preparation of the gradient structure of the bacterial cellulose membrane can be realized by adjusting the proportion of the crop starch to the thickening agent. The preparation method of the bacterial cellulose membrane disclosed by the invention has the advantages that the resources of raw materials are wide, biosynthesis process is mild and pollution-free, and the product is environment-friendly, safe, low in production cost, controllable in process and designable and has a bright large-scale production prospect. The bacterial cellulose membrane with the gradient structure is suitable for the fields of cosmetics and biomedical materials including a facial mask, a cooling paste, a wound dressing, an artificial skin and the like.

Disclosed herein plant propagation materials, methods of manufacturing, formulations and uses thereof. The plant propagation materials disclosed herein may comprise a strigolactone obtained by a biosynthetic process. The plant propagation material may comprise a chemical mimic of a strigolactone. The strigolactone may be 5-deoxystrigol. Methods of manufacturing the plant propagation materials may comprise a chemical process. Alternatively, methods of manufacturing the plant propagation material may comprise a biosynthetic process. The methods may comprise use of one or more polynucleotides. The polynucleotides may encode a metabolite. The polynucleotides may comprise one or more genes encoding one or more components of a strigolactone pathway.

The present invention describes the apparatus for aerobic liquid-phase fermentation. The present invention relates to the field of chemical, physical and physical-chemical processes carried out in apparatus with an aeration and intermixing of a liquid medium, namely: processes of biosynthesis of various biological products, processes of processing waste from various productions, processing of semi-products, and processes of clearing of waste water that could also can be used in food, medical, microbiological, petrochemical industries, and also in an ecological remediation of an environment from various wastes.

Disclosed herein plant propagation materials, methods of manufacturing, formulations and uses thereof. The plant propagation materials disclosed herein may comprise a strigolactone obtained by a biosynthetic process. The plant propagation material may comprise a chemical mimic of a strigolactone. The strigolactone may be 5-deoxystrigol. Methods of manufacturing the plant propagation materials may comprise a chemical process. Alternatively, methods of manufacturing the plant propagation material may comprise a biosynthetic process. The methods may comprise use of one or more polynucleotides. The polynucleotides may encode a metabolite. The polynucleotides may comprise one or more genes encoding one or more components of a strigolactone pathway.

The invention provides a spider dragline silk protein and a biosynthesis method thereof. The spider dragline silk protein comprises an 'ABA-type' triblock copolymer of a spider silk protein and a collagen-like protein, the amino acid sequence and the nucleotide sequence of the spider dragline silk protein are SEQ ID NO.1 and SEQ ID NO.2, and the collagen-like protein is a tripolymer (Pro-Gly-Pro)15 and positioned at two ends of the spider silk protein. The biosynthesis method includes the steps: (1) designing and optimizing a spider dragline silk protein gene; (2) building a gene expression vector; (3) converting, fermenting and culturing engineering bacteria; (4) inducing expression; (5) extracting and purifying products. The spider dragline silk protein has good biocompatibility, directing properties and fibroblast potential and can meet requirements of artificial spider silk fiber molecular weight, and the spider dragline silk protein is mild in biosynthetic process and simple in process and provided with a potential protein fiber material and has a broad prospect of industrial implementation.

The present inventors examined the inhibitory activity against HCV replicon of myriocin, fumonisin B1, and ceramide trafficking inhibitor HPA-12, which are compounds derived from microorganisms such as those of the genus Aureobasidium, and found that these compounds have the effect of inhibiting the replication of HCV replicon RNAs or inhibiting the expression of HCV proteins. The present inventors further performed serine palmitoyltransferase knockdown experiments using siRNAs, and results showed that HCV replicon activity and HCV protein expression were significantly inhibited in cells with suppressed LCB1 expression. Thus, sphingolipid biosynthesis was found to be involved in HCV infection. This showed that HCV infection can be treated or prevented by inhibiting enzyme activities involved in the process of sphingolipid biosynthesis by adding compounds or knocking down a gene.

The present invention aims to synthesize a polypeptide having an unnatural structure at the N-terminus via a biosynthetic process by translation of amino acid sequence information encoded by a nucleic acid. A polypeptide having any amino acid at the N-terminus is synthesized by using an ARS ribozyme that catalyzes the acylation of tRNA with any amino acid to attach any amino acid to an initiator tRNA, thereby initiating a translation with the initiator tRNA.

The invention relates to a Pavlova-viridis-derived delta-6 desaturase new gene. Polyunsaturated fatty acids, such as eicosapentaenoic acid (EPA) and dehydroacetic acid (DHA), are important nutrient substances which are necessary for the human body and can not be synthesized by the human body. In view of the microalga-Pavlova viridis capable of naturally synthesizing EPA and DHA, a conserved region sequence amplification-RACE combined method is used for for cloning to obtain the new delta-6 fatty acid desaturase gene. The acquisition of the gene provides references for further recognizing and utilizing the biosynthesis process of the EPA and DHA which have high practical significance.

The invention provides a compound shown as a formula I, or a conformational isomer thereof, or an optical isomer thereof, or a pharmaceutically acceptable salt thereof. The compound can be tightly combined with related binding proteins in an miRNA biosynthesis process and can effectively inhibit the synthesis of miRNA-21. The prepared active compound provided by the invention can be used as an miRNA-21 inhibitor, and further as a potential drug for treating malignant tumors. The formula I is shown in the description.

The invention discloses a biosynthesis method of perylenequinonoid compounds, in particular a biosynthesis method of perylenequinonoid compounds and specifically relates to optimization of a fermentation process in a biosynthesis process. A fungus, which grows in Yunnan western mountainous area and is identified to belong to ascomycetes, hypocreaceae and hypornyces, serves as a strain and a solid mixture, which contains maize powder, wheat bran and bean drges, is taken as a solid-state culture medium. The solid-state culture medium is sterilized and the strain is inoculated, and fermentation is conducted at 21-23 DEG C for no less than 9 days, so that a brick-red solid fermented product containing the perylenequinonoid compounds is obtained, therefore a bacterium block containing the perylenequinonoid is obtained through biosynthesis, wherein the bacterium block contains biosynthesis products of the invention, namely such perylenequinonoid compounds as elsinochrome A, hypocrellin A, hypocrellin B, hypomycin A and B, and the like. A finished perylenequinonoid compound product can be obtained by drying and crushing the bacterium block and by extracting and separating. According to the biosynthesis method disclosed by the invention, the total amount of the perylenequinonoid compounds in the finished product is improved.

The present invention relates to a polypeptide which belongs to the R2R3-type MYB family and which regulates the shikimate pathway towards the production of benzenoids. The shikimate pathway is a biosynthesis pathway through which the three essential aromatic amino acids tyrosine, phenylalanine and tryptophane are synthesized in plants, bacteria and fungi. The present inventiont provides for the first time a regulatory protein in the shikimate pathway and a means to regulate the biosynthesis of these three essential amino acids which cannot be produced by mammals. At the same time, it opens up the way for the regulation of the biosynthesis of aromatic and-non- aromatic compounds which are derived from these essential amino acids. A polypeptide or polynucleotide of the invention may be used in a method for manipulating the transcript levels of the genes of the shikimate pathway towards benzenoids for instance the genes of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (DAHPS), 5-enol-pyruvylshikimate-3-phosphate synthase (EPSPS), L- phenylalanine ammonia-lyase (PAL) and chorismate mutase (CM). Through these enzymes, biosynthetic processes at lower levels may be influenced. For instance, compounds of the invention may be used in a method to regulate scent in flowers or to regulate resistance to pest insects or pathogenic organisms.