Bacterial cellulose membrane with gradient structure and preparation method thereof

A technology of bacterial cellulose film and bacterial cellulose, applied in the field of bacterial cellulose film and its preparation, can solve the problems of decreased crystallinity of composite materials, agglomeration of starch granules, uneven distribution, etc., and achieves mild biosynthesis process and production efficiency. The effect of low cost and controllable preparation process

Active Publication Date: 2013-08-07
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Further research found that after adding starch, the crystallinity of the composite material decreased, and at the same time, the starch mainly covered the surface of the cellulose and the distribution was extremely uneven, and the starch granules were seriously agglomerated.
[0007] In the prior art, starch is added to the fermentation medium to make BC appear dense and loose and layered, but there are problems: the mechanism of starch building the gradient structure is not clear; the dense layer in the gradient structure is thin and the density cannot be adjusted. The ratio between the dense layer and the loose layer, there is obvious delamination between the dense layer and the loose layer, which affects the mechanical properties of the material; the dispersion uniformity of the starch in the composite material is poor and it mainly covers the surface of the cellulose

Method used

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  • Bacterial cellulose membrane with gradient structure and preparation method thereof
  • Bacterial cellulose membrane with gradient structure and preparation method thereof
  • Bacterial cellulose membrane with gradient structure and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] 1) the deployment of fermentation broth;

[0049] Components of the culture solution, in mass percent, in wt%: glucose, fructose, sucrose or mannitol 2, peptone 0.05, yeast extract 0.05, citric acid 0.01, disodium hydrogen phosphate 0.02, potassium dihydrogen phosphate 0.01, methyl Cellulose 0.1, wheat starch 0.1;

[0050] The pH of the culture solution is 4.0;

[0051] Heat the culture medium to 50°C to dissolve methyl cellulose and evenly disperse it in the culture medium, at the same time make the wheat starch gelatinize and evenly disperse in the fermentation medium, sterilize with high pressure steam, irradiate with ultraviolet light and cool to room temperature , through pure oxygen, the fermentation medium is obtained;

[0052] 2) strain expansion;

[0053] The fermentation culture liquid is inoculated and expanded; the degree of expansion: the number of cells of Acetobacter xylinum is 2×10 5 pieces / ml.

[0054] 3) Static cultivation;

[0055] Transfer the ...

Embodiment 2

[0059] 1) the deployment of fermentation broth;

[0060] Components of the culture solution, in mass percent, in wt%: glucose, fructose, sucrose or mannitol 3, peptone 0.2, yeast extract 0.2, citric acid 0.05, disodium hydrogen phosphate 0.1, potassium dihydrogen phosphate 0.05, methyl Cellulose 0.5, wheat starch 0.5;

[0061] The pH of the culture solution is 5.0;

[0062] Heat the culture medium to 70°C to dissolve the methylcellulose and disperse it uniformly in the culture medium, meanwhile, gelatinize the wheat starch and disperse it evenly in the fermentation medium, sterilize it with high pressure steam, irradiate it with ultraviolet light and cool it down to room temperature , through pure oxygen, the fermentation medium is obtained;

[0063] 2) strain expansion;

[0064] The fermentation culture liquid is inoculated and expanded; the degree of expansion: the number of cells of Acetobacter xylinum is 2×10 6 pieces / ml.

[0065] 3) Static cultivation;

[0066] Tran...

Embodiment 3

[0070] 1) the deployment of fermentation broth;

[0071] Components of the culture medium, in mass percent, in wt%: glucose, fructose, sucrose or mannitol 5, peptone 0.5, yeast extract 0.5, citric acid 0.1, disodium hydrogen phosphate 0.2, potassium dihydrogen phosphate 0.1, methyl Cellulose 1, Wheat Starch 1;

[0072] The pH of the culture solution is 6.0;

[0073] Heat the culture medium to 80°C to dissolve the thickener and evenly disperse it in the culture medium, and at the same time make the wheat starch gelatinize and evenly disperse in the fermentation medium, sterilize it with high pressure steam, irradiate it with ultraviolet light and cool it to room temperature. Through pure oxygen, the fermentation medium is obtained;

[0074] 2) strain expansion;

[0075] The fermentation culture liquid is inoculated and expanded; the degree of expansion: the number of cells of Acetobacter xylinum is 2×10 7 pieces / ml.

[0076] 3) Static cultivation;

[0077] Transfer the ex...

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Abstract

The invention discloses a bacterial cellulose membrane in a gradient structure and a preparation method of the bacterial cellulose membrane. The bacterial cellulose membrane is prepared by the steps of adding crop starch in a fermentation culture solution, heating for gelatinizing the mixture, adding a thickening agent into the gelatinized mixture, and carrying out static culture; and the bacterial cellulose membrane has a gradient structure with continuous transition from a compact upper surface downwards to a loose lower surface. The in-site fast controllable preparation of the gradient structure of the bacterial cellulose membrane can be realized by adjusting the proportion of the crop starch to the thickening agent. The preparation method of the bacterial cellulose membrane disclosed by the invention has the advantages that the resources of raw materials are wide, biosynthesis process is mild and pollution-free, and the product is environment-friendly, safe, low in production cost, controllable in process and designable and has a bright large-scale production prospect. The bacterial cellulose membrane with the gradient structure is suitable for the fields of cosmetics and biomedical materials including a facial mask, a cooling paste, a wound dressing, an artificial skin and the like.

Description

technical field [0001] The invention relates to a bacterial cellulose membrane with a gradient structure and a preparation method thereof, in particular to a bacterial cellulose membrane with a loose lower surface whose upper surface is dense and continuously transitions downward and a preparation method thereof. Background technique [0002] Bacterial Cellulose (also known as microbial cellulose) is a nanofibrous material. It uses the inside of the bacterial cell as a biosynthesis reactor, and the glucose small molecule undergoes a series of complex allosteric processes under the catalysis of the enzyme, and finally forms a β-1,4-glucose chain through the β-1,4-glucosidic bond. The catalytic site on the side of the lineage cell is extruded. The β-1,4-glucose chains interact with each other through intramolecular and intermolecular hydrogen bonds to form lipopolysaccharide layers, crystal-like aggregates, cellulose microfibrils, and finally cellulose in a stepwise and hiera...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C08L1/02C12R1/02
Inventor 王华平杨敬轩李喆吴旭君孙晓晓陈仕艳
Owner DONGHUA UNIV
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