Preparation method of rhodamine hybridized carbon dots and application of rhodamine hybridized carbon dots in mitochondrion target recognition
A targeted recognition and mitochondrial technology, which is applied in the field of intracellular mitochondrial targeted recognition, can solve the problems of short fluorescence emission wavelength, easy to be interfered by background light, etc., and achieve the effect of simple preparation, environmental friendliness and excellent fluorescence properties
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Embodiment 1
[0037] The preparation method of Rho-CDs 580 comprises the following steps:
[0038]Mix and dissolve 30 mg citric acid and 12.9 mg m-diethylaminophenol (molar ratio = 2:1) in 2 mL distilled water to prepare a precursor solution, and place it in a microwave reaction tube; place the microwave reaction tube in the microwave reaction tube In a container, the precursor solution was heated to 160 °C and reacted for 2 h; the obtained product was naturally cooled to room temperature; treated with a 0.22 μm filter membrane to remove large particles; and then treated with an ultrafiltration tube with a cutoff of 3 KDa to remove Purified Rho-CDs 580 solution was obtained from unreacted raw materials; the Rho-CDs 580 solution was vacuum freeze-dried to obtain Rho-CDs 580 solid powder. See the attached Figure 2-4 . Its electron microscope picture and particle size distribution chart are shown in the appendix Figure 5 and 6 , and its fluorescence emission diagrams under different exci...
Embodiment 2
[0040] The preparation method of Rho-CDs 520:
[0041] Mix 30 mg of citric acid and 8.5 mg of m-aminophenol (molar ratio = 2:1) and dissolve in 2 mL of distilled water to prepare a precursor solution. All the other steps are as in Example 1. See the attached Figure 2-4 .
Embodiment 3
[0043] The preparation method of Rho-CDs 540:
[0044] 30 mg of citric acid and 11.8 mg of m-ethylamino-p-cresol (molar ratio = 2:1) were mixed and dissolved in 2 mL of distilled water to prepare a precursor solution. All the other steps are as in Example 1. The UV-Vis absorption spectrum, excitation spectrum and fluorescence emission spectrum of Rho-CDs 540 solution are shown in the appendix Figure 2-4 .
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