Preparation method of rhodamine hybridized carbon dots and application of rhodamine hybridized carbon dots in mitochondrion target recognition

A targeted recognition and mitochondrial technology, which is applied in the field of intracellular mitochondrial targeted recognition, can solve the problems of short fluorescence emission wavelength, easy to be interfered by background light, etc., and achieve the effect of simple preparation, environmental friendliness and excellent fluorescence properties

Active Publication Date: 2018-09-07
ZHENGZHOU UNIV
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  • Abstract
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Problems solved by technology

However, for most carbon dots, their fluorescent emission wavelengths are generally short, mostly in the blue light range, and their applications in the biological field are susceptible to interference from background light

Method used

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  • Preparation method of rhodamine hybridized carbon dots and application of rhodamine hybridized carbon dots in mitochondrion target recognition
  • Preparation method of rhodamine hybridized carbon dots and application of rhodamine hybridized carbon dots in mitochondrion target recognition
  • Preparation method of rhodamine hybridized carbon dots and application of rhodamine hybridized carbon dots in mitochondrion target recognition

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Embodiment 1

[0037] The preparation method of Rho-CDs 580 comprises the following steps:

[0038]Mix and dissolve 30 mg citric acid and 12.9 mg m-diethylaminophenol (molar ratio = 2:1) in 2 mL distilled water to prepare a precursor solution, and place it in a microwave reaction tube; place the microwave reaction tube in the microwave reaction tube In a container, the precursor solution was heated to 160 °C and reacted for 2 h; the obtained product was naturally cooled to room temperature; treated with a 0.22 μm filter membrane to remove large particles; and then treated with an ultrafiltration tube with a cutoff of 3 KDa to remove Purified Rho-CDs 580 solution was obtained from unreacted raw materials; the Rho-CDs 580 solution was vacuum freeze-dried to obtain Rho-CDs 580 solid powder. See the attached Figure 2-4 . Its electron microscope picture and particle size distribution chart are shown in the appendix Figure 5 and 6 , and its fluorescence emission diagrams under different exci...

Embodiment 2

[0040] The preparation method of Rho-CDs 520:

[0041] Mix 30 mg of citric acid and 8.5 mg of m-aminophenol (molar ratio = 2:1) and dissolve in 2 mL of distilled water to prepare a precursor solution. All the other steps are as in Example 1. See the attached Figure 2-4 .

Embodiment 3

[0043] The preparation method of Rho-CDs 540:

[0044] 30 mg of citric acid and 11.8 mg of m-ethylamino-p-cresol (molar ratio = 2:1) were mixed and dissolved in 2 mL of distilled water to prepare a precursor solution. All the other steps are as in Example 1. The UV-Vis absorption spectrum, excitation spectrum and fluorescence emission spectrum of Rho-CDs 540 solution are shown in the appendix Figure 2-4 .

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Abstract

The invention relates to a preparation method rhodamine hybridized carbon dots and application of the rhodamine hybridized carbon dots in mitochondrion target recognition. The preparation method disclosed by the invention has the beneficial effects that a microwave assisted method is utilized, citric acid and an m-amino phenol compound are used as raw materials, fluorescence emission wavelength can be regulated and controlled through a way of changing an amino group in a meta position of phenol, rhodamine molecular fluorogen hybridized carbon dots are generated, and preparation of controllableand divinable fluorescence emission wavelength carbon dots is realized. The preparation method disclosed by the invention is simple in operation, can easily obtain the raw materials and is green andenvironment-friendly, and a new strategy for synthesizing molecular fluorogen hybridized carbon dots is created. The rhodamine hybridized carbon dots prepared by the invention can be used for the mitochondrion target recognition in cells, and has potential application value in the fields of biology, medical treatment and the like.

Description

technical field [0001] The invention relates to the field of nanomaterial manufacturing, in particular to a method for preparing rhodamine hybridized carbon dots by using a microwave-assisted method and a method for using the nanomaterial for intracellular mitochondrial targeting recognition. Background technique [0002] After more than ten years of development, carbon dots (CDs), as a new type of photoluminescent nanomaterial, have been widely used in biomarkers, cancer diagnosis, fluorescence sensing, solar cells, etc. due to their unique photoelectric properties. application. Compared with traditional semiconductor quantum dots (CdTe, CdSe, PbS), carbon dots have become a research hotspot in the field of biomarkers due to their advantages such as low toxicity, environmental friendliness, strong photostability, and simple preparation methods, and are considered to be a The most ideal fluorescent labeling and detection material. [0003] In the "bottom-up" synthesis meth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06C09K11/65B82Y20/00B82Y30/00B82Y40/00G01N21/64
CPCB82Y20/00B82Y30/00B82Y40/00C09K11/06C09K11/65C09K2211/1007C09K2211/1088G01N21/6486
Inventor 李朝辉耿欣孙远强屈凌波
Owner ZHENGZHOU UNIV
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