Biosynthetic method and application of bulbus fritillariae cirrhosae endophytic fungus mediated nano-silver

Abstract

The invention belongs to the technical field of biosynthesis and discloses a biosynthetic method and an application of bulbus fritillariae cirrhosae endophytic fungus mediated nano-silver. A bulbus fritillariae cirrhosae endophytic fungus soak solution and a silver nitrate solution are subjected to a reaction, and nano-silver 4-AgNPs and 13-AgNPs are prepared from bulbus fritillariae cirrhosae endophytic fungi CBY4 and CBY13 with the biosynthetic method after 15 bulbus fritillariae cirrhosae endophytic fungi are screened primarily; in the biosynthetic process, the synthetic conditions are as follows: pH is 3-11, concentration of the silver nitrate solution is 2 mmol/L, illumination intensity is 2000 Lux, and the temperature is 50 DEG C. It is discovered for the first time that the endophytic fungi CBY4 and CBY13 separated from bulbus fritillariae cirrhosae have nano-silver synthesizing capacity, the reaction conditions more suitable for nano-silver synthesis are obtained by optimization, and synthesized nano-silver has certain bacteriostatic and antitumor activity.

Description

technical field [0001] The invention belongs to the technical field of biosynthesis, and in particular relates to a biosynthesis method and application of nano-silver mediated by endophytic fungi of Fritillaria sichuanensis. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: [0003] 1. Analysis status of endophytic fungi [0004] Endophytic fungi are a group of fungi that live between or within the tissue cells of healthy plants without causing obvious disease symptoms. Since Vogl and other scientists successfully isolated the first strain of endophytic fungi from the seeds of ryegrass in 1898, the analysis of endophytic fungi has never stopped in the past 120 years. At present, the analysis of endophytic fungi has covered the entire plant kingdom. Whether it is common lower plants such as mosses and lichens, or higher plants with high economic value such as ginseng and Dendrobium candidum. Especially in 1993...

AI Technical Summary

Problems solved by technology

[0038] 2.2.2.1.1 Bacteria

The synthesis of nano silver in cells mainly uses some biological enzymes in cells as reducing agents to reduce silver ions into nano silver particles, but the mechanism is not yet clear.

Moreover, to obtain nano-silver synthesized in cells, it is necessary to first use ultrasonic waves or cell lysing agents to break up the cells, which increases the difficulty of downstream nano-silver recovery and purification.

[0047] There are three main methods for extracellular synthesis of nano-silver: (1) Using microbial cells, centrifuge or filter the cultured microbial culture solution, discard the supernatant, collect the cells, and repeatedly wash the cells with distilled water to remove surface interfering substances , react with silver nitrate solution, silver ions will induce bacteria to produce active substances that can reduce silver ions, but the synthesis process is slow

(2) adopt microbial culture supernatant, with the centrifugation of cultured microbial culture, get supernatant and silver nitrate solution reaction, the active substance that contains in the supernatant can reduce silver ion to nano-silver particle, but supernatant The components contained in the liquid are complex, and the cost of separation and purification of nano-silver is high

(3) Interaction with sulfhydryl groups leads to inactivation of key bacterial enzymes

[0064] The purification and separation of monomers is difficult, and it is difficult to completely separate cell metabolites and nano silver

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