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Protein related to Perilla frutescens L. terpene substance biosynthesis, encoding gene of protein, and application

A technology of biosynthesis and encoding genes, applied in the fields of plant genetic improvement, application, genetic engineering, etc., can solve the problem that few people have studied

Inactive Publication Date: 2021-05-04
河北省农林科学院经济作物研究所
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

At present, relevant research at home and abroad mainly focuses on the development and utilization of perilla, important agronomic traits of germplasm resources, planting and cultivation techniques, and oil content analysis. Pathways and gene function analysis, etc., are rarely studied

Method used

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  • Protein related to Perilla frutescens L. terpene substance biosynthesis, encoding gene of protein, and application
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  • Protein related to Perilla frutescens L. terpene substance biosynthesis, encoding gene of protein, and application

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Embodiment Construction

[0035] The technical solution of the present patent will be described in further detail below in conjunction with specific embodiments.

[0036] Embodiments of the present patent are described in detail below, examples of which are illustrated in the accompanying drawings, wherein the same or similar reference numerals refer to the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present patent, but should not be construed as a limitation on the present patent.

[0037] 1. Acquisition and functional verification of proteins related to terpenoid biosynthesis and their encoding genes

[0038] The acquisition of proteins related to terpenoid biosynthesis and their encoding genes

[0039] (1) Cloning of Perilla frutescens L. PfGPPS protein cDNA

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Abstract

The invention discloses a protein related to Perilla frutescens L. terpene substance biosynthesis, an encoding gene of protein, and application, and is the protein consisting of an amino acid sequence disclosed by a sequence 2 in a sequence table, and a gene which encodes the protein related to the Perilla frutescens L. terpene substance biosynthesis, and the encoding sequence of the gene is a DNA molecule disclosed by a sequence 1 in the sequence table. The invention provides a pfGPPS protein and an encoding gene thereof, the encoding gene is imported into tobaccos to obtain a tobacco plant which carries out overexpression on a pfGPPS gene, then, the transgenosis tobacco plant is subjected to pot culture, during a mature period, transgenosis tobacco leaves are harvested, and the content of terpene substances is detected through GC-MS. Compared with wild tobaccos, the content of the terpene substances in the transgenosis tobacco is obviously improved. Therefore, the pfGPPS gene and the protein encoded by the pfGPPS gene perform an important function in a plant terpene substance biosynthesis process. The pfGPPS gene provided by the invention and the protein encoded by the pfGPPS gene have an important application value in research of improvement of the content of the plant terpene substances.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a protein related to the biosynthesis of perilla terpenoid substances, and its encoding gene and application. Background technique [0002] Perilla frutescens L. is an annual herbal medicinal plant of the Lamiaceae family, native to eastern Asia, divided into wild type and cultivated type, widely distributed in various provinces and regions in my country, as well as Bhutan, Indochina, Indonesia, Java, Japan , North Korea and other places. Perilla has been cultivated in my country for more than 2,000 years. In the north, it is mainly used for oil, and it is also used for medicinal purposes. There are two traditional oil producing areas in the northwest and northeast. Perilla is one of the 87 crops announced by the National Health Commission in 2019 as both a drug and a food. It has important development value in the fields of medicine and food, and has received extensive attention at ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/10C12N15/54C12N15/84C12N15/11A01H6/50A01H5/12A01H6/82
CPCC12N9/1085C12N15/8243C12Y205/01001
Inventor 姜涛温春秀刘灵娣温赛群田伟谢晓亮
Owner 河北省农林科学院经济作物研究所
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