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Method of saccharomycetes bio-synthesing, producing optical pure adenosine methionine and its culture solution

A pure adenosylmethionine biosynthesis technology, applied in fermentation, fungi, etc., can solve the problems of difficult large-scale preparation and high production costs, and achieve simple and easy preparation methods, low raw material costs, and easy scale The effect of chemical production

Inactive Publication Date: 2007-05-02
SHANGHAI DUOMIRUI BIOLOGICAL TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

U.S. Patent [DN: 20020025926A1] discloses a separation and purification method for optically pure (S, S)-type adenosylmethionine. Since the method needs to use preparative high-performance liquid chromatography in production, the production cost High, and it is not easy to achieve large-scale preparation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The enrichment of yeast cells can be produced by using conventional yeast fermentation methods, or can be entrusted to yeast fermentation production enterprises. Take Saccharomyces carlsbergensis CBS 1513 wet bacteria 500kg in 2.0m 3 In the bioreactor, add 500kg tap water, 50kg glucose (concentration 5%), 2kg yeast extract (concentration 0.2%), 3kg dipotassium hydrogen phosphate (concentration 0.3%) and 10kg DL-type methionine (concentration 1.0%) to form 1000L reaction solution, the stirring speed is 180rpm, the ventilation rate is 0.5vvm, and the temperature is controlled at 27°C. After 12h of reaction initiation, the concentration of glucose is maintained at 0.8% by feed feeding and maintained for 10h. Sampling and analysis resulted in 11.6 g / l of adenosylmethionine.

Embodiment 2～3

[0036] Sucrose and molasses were used as carbon sources to replace the glucose in Example 1, and the remainder was the same as in Example 1. As a result, 8.6 g / l and 10.5 g / l of adenosylmethionine were obtained respectively.

Embodiment 4

[0038] Change the concentration of glucose in Example 1 to be 1%, add the nitrogen source peptone that the concentration is 2% in the culture solution, the yeast extract concentration is 0.05%, the concentration of methionine is 2%, and the air flow is at 0.2vvm, The temperature was controlled at 37°C, the reaction time was 18 hours, and the glucose concentration maintained by feeding was 1% to the end of the reaction after 8 hours from the start of the reaction. The rest was the same as in Example 1, and the result was 7.2g / l of adenosine methyl sulfide. acid.

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Abstract

This invention provides a yeast fungus biosynthesis preparation of optical rotation adenosyl methionine. Biosynthesis of adenosyl methionine is conducted using yeast fungus by importing air into nutrient solution containing carbon source, methionine and vitamin, stirring and add carbon source during the biosynthetic process. This invention also discloses a preparation of producing optical rotation adenosyl methionine with above-mentioned method, and provides a nutrient solution for yeast fungus biosynthetic optical rotation adenosyl methionine. The level of biosynthetic adenosyl methionine will reach 10g / l, and the purity of the products is over 98.5%, with containing more than 97.0% (S,S)-type. This method is simple and of low cost, so it is easy to produce in great scale.

Description

technical field [0001] The invention relates to a method for producing adenosylmethionine by biosynthesis, in particular to a method for large-scale synthesis by yeast and further production of (S, S)-type optically active pure adenosylmethionine method, and a culture medium used therein. Background technique [0002] Adenosylmethionine is also known as adenosylmethionine, active methylthioadenosine, and the English abbreviation is SAMe or SAM. It is the main methyl donor in the human body and other organisms. Vitamin B 12 Provide methyl, provide aminopropyl for polyamine synthesis in vivo, provide precursors for tRNA biosynthesis, participate in the biosynthesis and metabolism of hormones, neurotransmitters, nucleic acids, proteins and phospholipids in vivo, and maintain the normal function of cell membranes and normal human body An important life substance indispensable for metabolism and health. Adenosylmethionine has clear curative effects on liver disease, depression...

Claims

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Application Information

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IPC IPC(8): C12P19/44C12N1/16
Inventor 冯军赵文杰程晴华朱裕辉薛春佳蒋家琛林纲
Owner SHANGHAI DUOMIRUI BIOLOGICAL TECH CO LTD
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