Clostridium butyricum having diarrhea treating effect and application of clostridium butyricum as substitute of sodium butyrate

A Clostridium butyricum, substitute technology, applied in the direction of application, bacteria, microbial-based methods, etc., can solve the problems of small dissociation degree, reduced feed intake, high technical cost, etc., achieve strong acid production ability and repair mucous membrane , the effect of broad application prospects

Active Publication Date: 2012-07-11
山东宝来利来生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] (2) The amount of sodium butyrate is not easy to control: as an organic acid, sodium butyrate has a small degree of dissociation and weak acidity. The amount of sodium butyrate to achieve the same acidification capacity is much larger than that of inorganic acids, and the cost of addition is high.
[0009] (3) Storage and transportation conditions are limited: Sodium butyrate products are stable at low temperature and in a sealed state under normal storage and handling conditions, but they cannot be mixed with substances of the opposite nature, dust, overheated items, and strong oxidants. It is easy to dilute and agglomerate or cause the feed to be damp
[0010] (4) High technical cost: The results of studying the absorption of SCFA (short-chain fatty acid) by nuclide tracer met...

Method used

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  • Clostridium butyricum having diarrhea treating effect and application of clostridium butyricum as substitute of sodium butyrate
  • Clostridium butyricum having diarrhea treating effect and application of clostridium butyricum as substitute of sodium butyrate
  • Clostridium butyricum having diarrhea treating effect and application of clostridium butyricum as substitute of sodium butyrate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Isolation, identification and culture characteristics of 1 strain

[0041] 1 Materials and methods

[0042] 1.1 Sample collection The feces of healthy sows in Ningyang Ecological Ranch, Tai'an City, Shandong Province.

[0043] 1.2 Medium

[0044] Clostridium proliferation medium: tryptone 5g / L, beef extract 5g / L, yeast extract 3g / L, glucose 10g / L, K 2 HPO 4 2g / L, MgSO 4 0.2g / L, CaCO 3 10g / L, MnSO 4 0.2g / L; pH 7.0, 121°C, 15min sterilization.

[0045] Clostridium count medium: tryptone 5g / L, beef extract 5g / L, yeast extract 3g / L, glucose 10g / L, K 2 HPO 4 2g / L, MgSO 4 0.2g / L, CaCO 3 10g / L, MnSO 4 0.2g / L, agar 15g / L; pH 7.0, 121℃, 15min sterilization.

[0046] Clostridium isolation medium: add to the proliferation medium: taurocholate 5g / L, agar 15g / L, adjust the pH to 7.0, and sterilize for 15 minutes.

[0047] 1.3 Separation method

[0048] 1) Take 10 g of feces samples and add them to 90 mL of sterile water, put them in an 80°C water bath...

Embodiment 2

[0200] Example 2 Application of Clostridium butyricum Cb-2 instead of sodium butyrate in piglet production

[0201] purpose meaning

[0202] Piglet weaning syndrome has always been an important problem restricting the healthy cultivation of piglets. The current common method is to add organic acids to the diet after weaning. Sodium butyrate is a relatively stable sodium salt of butyric acid, which plays an important role in treating piglet diarrhea, improving gastrointestinal function, and improving animal production performance.

[0203] Clostridium butyricum is a kind of probiotic bacteria in the intestinal tract of animals. It has been reported as a micro-ecological feed additive. At the same time, because this bacteria can form spores, it has strong resistance to the external environment. It has strong stress resistance and tolerance. High temperature and high pressure and resistance to a variety of antibiotics have become the hotspots of research and application today. ...

Embodiment 3

[0225] Example 3 Clostridium butyricum Therapeutic application test to mice with diarrhea

[0226] Objective: To observe the effect of Clostridium butyricum Cb-2 on ampicillin-induced diarrhea and intestinal flora in mice, and to evaluate the role of Clostridium butyricum Cb-2 in regulating the balance of intestinal flora.

[0227] Methods: Ampicillin gavage method was used to create a mouse model of diarrhea; after successful model establishment, different doses of Clostridium butyricum Cb-2 were fed for treatment, and the feces at different treatment times were observed and detected for Bifidobacterium, Lactobacillus, Number of Enterobacteriaceae, Enterococci and Bacteroidetes.

[0228] 1 Materials and methods

[0229] 1.1 Experimental animals: Kunming mice, male, weighing 18-22 g, purchased from Thailand Institute of Biological Products.

[0230] 1.2 Medicines: Ampicillin, purchased from Kangdien Biopharmaceutical Co., Ltd.

[0231] 1.3 Medium: BDS, LBS, eosin methylene ...

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Abstract

The invention discloses a clostridium butyricum having a diarrhea treating effect. The strain is named as clostridium butyricum Cb-2 which is conserved in China Center for Type Culture Collection with the conservation number of CCTCC M2011384, in November 9th, 2011. A test proves that the clostridium butyricum disclosed by the invention has strong acid producing capability especially butyric acidproducing capability; and the clostridium butyricum has obvious effects on diseases, such as intestinal tract flora infection, acute and chronic diarrhea, irritable bowel syndrome and non-ulcer dyspepsia, caused by various reasons, and has no toxicity and harm. Thus, the clostridium butyricum or clostridium butyricum powder disclosed by the invention is used as a raw material for preparing a micro-ecological preparation and used for replacing sodium butyrate to be applied; and when a specific application is carried out, a clostridium butyricum preparation can be singly used as an active component to be prepared into the micro-ecological preparation together with corn starch, or, the clostridium butyricum preparation is mixed with at least one of other bacterial preparations and the corn starch to prepare a composite micro-ecological preparation.

Description

technical field [0001] The invention relates to a Clostridium butyricum strain capable of treating diarrhea and its application as a sodium butyrate substitute. Background technique [0002] At present, with the continuous development of the economy and the continuous improvement of living standards, people have increasingly realized the basic meaning of "green". However, due to the drive of economic interests, the use of antibiotics has begun to flood, especially in large quantities in feed, which leads to the imbalance of normal flora in animals and inhibits the formation of the body's immune response, leading to serious superinfection and even death. Secondly, the residues of antibiotics in livestock and poultry and their meat, eggs, milk and other foods cannot be completely inactivated by heating. People who eat such foods for a long time may cause chronic poisoning. In addition, modern medical technology, pesticides, fertilizers, hormones, isotopes and other chemical p...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/16C12R1/145
Inventor 单宝龙谷巍王红翟延庆郭洪新
Owner 山东宝来利来生物工程股份有限公司
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