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Clostridium butyricum

A technology of caproic acid bacteria and strains, applied in the field of microorganisms, can solve the problems of shortening the aging time of new cellars, achieve stable quality and flavor, obvious effects, and reduce business expenses

Inactive Publication Date: 2011-01-12
HUBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The strain has the characteristics of high caproic acid production and good growth stability. After strengthening the functional bacteria, it can be used to prepare high-concentration compound caproic acid bacterial liquid, which can be applied to the cultivation of artificial pit mud, and can shorten the aging of newly built cellars in actual production. The effect of cycle and rejuvenation of degraded cellar function improves the yield rate and high-quality product rate, solves the problems of rejuvenation of degraded old cellars and shortens the aging time of new cellars

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0031] The preparation of embodiment 1 caproic acid bacteria (Clostridium butyricum) GK13

[0032] Clostridium butyricum GK13 is a strain of Clostridium isolated from the high-quality old pit mud of Luzhou-flavor liquor. The isolation medium (EAM medium) ethanol 2.0% (after sterilization and before use), NaAc0.5 %, Yeast 0.1%, MgSO 4 ·7H 2 O 0.02%, K 2 HPO 4 0.04%, (NH 4 ) 2 SO 4 0.05%, CaCO 3 1.0%, natural pH; add 3.0% agar to solid medium. The pit mud samples were taken through heat treatment at 80°C, enriched and cultivated for copper sulfate color development for primary screening, and samples with good acid and gas production conditions were selected for further screening, and the products were qualitatively and quantitatively analyzed by gas chromatography, and caproic acid was selected. The samples with the best level were separated and purified. Take 1mL of re-screening sample solution, add 10ml of sterile water, shake for 1min, take a small amount of liquid f...

Embodiment 2

[0033] The preparation of embodiment 2 compound caproic acid bacterial strains

[0034] The caproic acid bacteria (Clostridium butyricum) GK13 strain, compound methanogens and butyric acid bacteria were strengthened, and the compound strain was rejuvenated by heat treatment at 80°C to prepare a high-concentration compound caproic acid bacteria strain ZC-21. After strengthening, the acid production level of ZC-21 and the total number of spore-producing bacteria have been greatly improved, and the analysis of the results of multiple subcultures shows that the acid production level of the strain and the growth of spores have not degenerated. Has good stability. The test results are shown in Table 1.

[0035] Table 1 The levels of acid production and spore production of compound caproic acid bacteria strain ZC~21

[0036]

[0037] Example 3 Application of the compound caproic acid bacteria strain ZC~21 prepared in Example 2 in artificial pit mud cultivation

[0038] (1) Arti...

Embodiment 4

[0055] Example 4 Application of compound caproic acid bacteria strain ZC~21 in the rejuvenation of degenerated old cellars:

[0056] (1) Formula and preparation method of rejuvenation culture medium

[0057] ZC~21 compound caproic acid bacteria liquid 25~30kg, high-quality pit mud (sterilized at normal pressure for half an hour) 10~15kg, loess 30kg, 20° wine tail 15~20kg, washing water (boiling to remove slag) 13~20kg, Daqu powder 6kg, aromatic yeast (activated in warm water at 32-35°C for 2 hours) 300g, sodium acetate 130g, dipotassium hydrogen phosphate 50g, ammonium phosphate 50g, esterified red yeast rice 475g.

[0058] Stir the above raw materials into a paste, adjust the alcohol content to 2%, pH 6-pH 6.5, culture product temperature 32-35°C, stir once on the 2nd day, seal the culture for about 7 days, and then use it.

[0059] (2) Rejuvenation method of degraded cellar

[0060] The pit mud of the aging cellar was carefully tested and analyzed. Determination of pit mu...

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Abstract

The invention aims to provide a Clostridium butyricum GK13 bacterial strain CCTCCNO. M2010175. The bacterial strain has the characteristics of high caproic acid yield and good growth stability, can be applied in preparing high-concentration compound Clostridium butyricum liquid after strengthening functional bacteria, can be applied in cultivating artificial pit mud, obtains the effect of shortening the newly-built cellar pool aging period and rejuvenating the degrade cellar pool function in the practical production, improves the alcohol yield and the superior product rate, and solves the problems of rejuvenating a degrade old cellar pool, and shortening the newly-built cellar pool aging time and the like.

Description

technical field [0001] The invention relates to a microorganism, in particular to a high-caproic acid-producing bacterial strain that can be applied to artificial pit mud cultivation and degraded old pit pond maintenance, rapidly increasing the number of main functional bacteria in pit pit mud, and shortening pit maturation time. Background technique [0002] The fermentation of Luzhou-flavor Daqu liquor adopts a unique mud cellar fermentation process, which belongs to the co-fermentation of various microbial groups and is a complex biochemical reaction system. The study found that ethyl caproate is the main aroma substance in Luzhou-flavor liquor, and the content of ethyl caproate directly affects the quality of Daqu liquor. It comes from the combination of caproic acid produced by a class of Clostridium-caproic acid bacteria that inhabits the pit mud and ethanol produced by yeast in the fermented grains. During the fermentation process, the formation of caproic acid and e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12G3/02C12R1/145
Inventor 陈茂彬镇达方尚玲汪江波
Owner HUBEI UNIV OF TECH
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