Preparation method and application of composite microbial feed additive

Abstract

The invention discloses a preparation method and application of a composite microbial feed additive, belonging to the technical field of microbial engineering. The composite microbial feed additive provided in the invention comprises a live Clostridium butyricum preparation and a live Bacillus licheniformis preparation, wherein the live Clostridium butyricum preparation is obtained by the steps of seed culturing in triangular flasks, culturing in a seeding tank, culturing in a fermentation cylinder, bacterial sludge collecting by centrifugation, mixing with calcium carbonate, drying at low temperature, crushing, sieving and mixing with the accessory starch, and the live Bacillus licheniformis preparation is obtained by the steps of strain culturing on an inclined plane, culturing in eggplant-like flasks, culturing in a seeding tank, culturing in a fermentation cylinder, bacterial sludge collecting by centrifugation, mixing with calcium carbonate, drying at low temperature, crushing, sieving and mixing with the accessory starch. The composite microbial feed additive is obtained by evenly mixing the live Clostridium butyricum preparation and the live Bacillus licheniformis preparation according to a mass ratio of 1:1. The composite microbial feed additive can substantially raise the utilization rate of a feed, significantly reduce cultivation cost and increase economic benefits.

Description

technical field [0001] The invention relates to a preparation method and application of a compound microbial feed additive, belonging to the technical field of microbial engineering. Background technique [0002] With the progress of society and the improvement of people's living standards, food safety issues have attracted more and more attention in recent years. Especially in 2008, melamine was found in milk powder such as Sanlu, and melamine was found in eggs later, food safety is even more worrying. Through follow-up investigations, it was found that in addition to artificially added melamine in food, some of it was caused by animals eating feed containing melamine. Therefore, food safety must first start with feed safety. [0003] In my country, for a long time, in the process of animal husbandry and aquaculture, it has been relying on adding antibiotics to feed to prevent and control animal diseases. Almost all feeds sold in the market are added with antibiotics. Du...

AI Technical Summary

Problems solved by technology

Lactic acid bacteria are beneficial bacteria in the intestinal tract of animals. They can colonize the intestinal tract and play a good role in regulating the intestines. However, since lactic acid bacteria do not have spores, these preparations have poor storage stability, cannot be stored at room temperature for a long time, and are not resistant to gastric acid. During the process, it will lose a large part of its activity when it passes through the stomach, especially when it is added to the feed for granulation, the bacteria cannot withstand high temperatures and die in large numbers, which affects the promotion and application of the product. Acid-resistant and high-temperature-resistant Bacillus products

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