Preparation method and application of composite microbial feed additive

A technology of feed additives and compound microorganisms, applied in the field of microbial engineering, can solve problems such as inability to store at room temperature for a long time, affect product promotion and application, and poor storage stability, so as to regulate intestinal microecological balance, enhance animal immunity, and promote proliferation and colonization effects

Inactive Publication Date: 2011-10-26
JIANSGU INST OF MICROBIOLOGY
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  • Claims
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Problems solved by technology

Lactic acid bacteria are beneficial bacteria in the intestinal tract of animals. They can colonize the intestinal tract and play a good role in regulating the intestines. However, since lactic acid bacteria do not have spores, these preparations have poor storage stability, cannot be stored at room temperature for a long time, and are not resistant to gast...

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  • Preparation method and application of composite microbial feed additive
  • Preparation method and application of composite microbial feed additive

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Embodiment 1

[0067] The preparation method of the compound microbial feed additive, the preparation steps are as follows:

[0068](1) Preparation of Clostridium butyricum live bacteria preparation

[0069] a. Erlenmeyer flask seed culture: Prepare 2200mL of liquid medium according to the formula, adjust the pH to 6.5~7.5, and divide into Erlenmeyer flasks, 100mL in 150mL Erlenmeyer flask, 1900mL in 2000mL Erlenmeyer flask, sterilize at 121°C for 30 minutes, and sterilize for 30 minutes after cooling. CGMCC No.4664 freezing tube was inoculated into a 150mL triangular flask, and cultured at 30~38°C under anaerobic conditions for 14 hours; the grown Clostridium butyricum seeds were transferred into a 2000mL triangular flask with 5% inoculum In the bottle, culture statically at 30~38°C for 24 hours.

[0070] b. Seed tank cultivation: Add liquid culture medium to a 50L seed tank with a liquid volume of 70%, adjust the pH to 7.0~8.5, start stirring, sterilize at 121°C for 30 minutes, turn on co...

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Abstract

The invention discloses a preparation method and application of a composite microbial feed additive, belonging to the technical field of microbial engineering. The composite microbial feed additive provided in the invention comprises a live Clostridium butyricum preparation and a live Bacillus licheniformis preparation, wherein the live Clostridium butyricum preparation is obtained by the steps of seed culturing in triangular flasks, culturing in a seeding tank, culturing in a fermentation cylinder, bacterial sludge collecting by centrifugation, mixing with calcium carbonate, drying at low temperature, crushing, sieving and mixing with the accessory starch, and the live Bacillus licheniformis preparation is obtained by the steps of strain culturing on an inclined plane, culturing in eggplant-like flasks, culturing in a seeding tank, culturing in a fermentation cylinder, bacterial sludge collecting by centrifugation, mixing with calcium carbonate, drying at low temperature, crushing, sieving and mixing with the accessory starch. The composite microbial feed additive is obtained by evenly mixing the live Clostridium butyricum preparation and the live Bacillus licheniformis preparation according to a mass ratio of 1:1. The composite microbial feed additive can substantially raise the utilization rate of a feed, significantly reduce cultivation cost and increase economic benefits.

Description

technical field [0001] The invention relates to a preparation method and application of a compound microbial feed additive, belonging to the technical field of microbial engineering. Background technique [0002] With the progress of society and the improvement of people's living standards, food safety issues have attracted more and more attention in recent years. Especially in 2008, melamine was found in milk powder such as Sanlu, and melamine was found in eggs later, food safety is even more worrying. Through follow-up investigations, it was found that in addition to artificially added melamine in food, some of it was caused by animals eating feed containing melamine. Therefore, food safety must first start with feed safety. [0003] In my country, for a long time, in the process of animal husbandry and aquaculture, it has been relying on adding antibiotics to feed to prevent and control animal diseases. Almost all feeds sold in the market are added with antibiotics. Du...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/16C12R1/145C12R1/10A23K10/18
Inventor 唐宝英张六六吴燕刘佳陆茂林
Owner JIANSGU INST OF MICROBIOLOGY
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