Method for preparing active dietary fibers through fermentation of irpex lacteus and schizophyllum commune

A technology for Raketodon albicans and Schizophyllum, which is applied in the field of degrading and transforming potato pomace to synthesize active dietary fiber, can solve the problems such as failure to fully break, refractory lignocellulose and degrading microorganisms, etc. Effects of immunity, high medical value

Active Publication Date: 2018-01-12
孙敏
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the refractory nature of lignocellulose and the particularity of degrading microorganisms, this technology has not yet been well established. In my country, the technology of microbial fermentation to produce active dietary fiber is limited to the laboratory research stage.

Method used

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  • Method for preparing active dietary fibers through fermentation of irpex lacteus and schizophyllum commune
  • Method for preparing active dietary fibers through fermentation of irpex lacteus and schizophyllum commune
  • Method for preparing active dietary fibers through fermentation of irpex lacteus and schizophyllum commune

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0090] Embodiment 1: a kind of preparation method of albicans albicans PCV1, comprises the following steps:

[0091] The sterile water used for soil in Shennongjia, Hubei Province was divided into 10 -2 ~10 -6 After gradient dilution, spread on potato dextrose agar medium, culture at 25°C for 24 hours, pick out a single colony and dissolve it in 10mL sterile water, and make 10 -2 ~10 -6 Bacterial solution of gradient concentration, each draws 0.15mL in potato dextrose agar medium, cultivates at 25 ℃ for 24 hours, described potato dextrose agar medium is potato 200g, glucose 20g, agar 20g, tap water 1000mL, natural pH; Bacterial colonies were further inoculated in starch degradation selection medium, and cultured at 25°C for 5 days; the starch degradation selection medium was 200g of potatoes, 20g of glucose, 20g of agar, 1000mL of tap water, natural pH, 15g of starch, and 2mL of iodine solution; screening Several strains of fungi with strong starch-degrading ability were ob...

Embodiment 2

[0094] Embodiment 2: a kind of preparation method of albicans albicans PCV1, comprises the following steps:

[0095] The sterile water used for soil in Shennongjia, Hubei Province was divided into 10 -2 ~10 -6 After gradient dilution, spread on potato dextrose agar medium, culture at 25°C for 36 hours, pick out a single colony and dissolve it in 10mL sterile water, and make 10 -2 ~10 -6Bacterial solution of gradient concentration, each draws 0.20mL on potato dextrose agar medium, cultures at 25 ℃ for 48 hours, described potato dextrose agar medium is potato 250g, glucose 25g, agar 25g, tap water 1000mL, natural pH; Bacterial colonies were further inoculated in starch degradation selection medium, and cultured at 25°C for 6 days; the starch degradation selection medium was 250g of potatoes, 25g of glucose, 25g of agar, 1000mL of tap water, natural pH, 20g of starch, and 3mL of iodine solution; Several strains of fungi with strong starch-degrading ability were obtained; then,...

Embodiment 3

[0097] The screening of the effective degrading potato dregs strain: add 60 g water to 100 g wet potato dregs after beating, add fungal strains according to the inoculation amount of 10%, culture at 25°C for 6 days, and detect the liquefaction, starch degradation and cellulose degradation of the culture system situation. Embodiment 3: a kind of preparation method of albicans albicans PCV1, comprises the following steps:

[0098] The sterile water used for soil in Shennongjia, Hubei Province was divided into 10 -2 ~10 -6 After gradient dilution, spread on potato dextrose agar medium, culture at 28°C for 48 hours, pick out a single colony and dissolve it in 10mL sterile water, and make 10 -2 ~10 -6 Bacterial solution of gradient concentration, each draw 0.20mL in potato dextrose agar medium, cultivate 72 hours at 28 ℃, described potato dextrose agar medium is potato 225g, glucose 23g, agar 23g, tap water 1000mL, natural pH; Bacterial colonies were further inoculated in starc...

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Abstract

The invention relates to novel strains, namely, irpex lacteus (Irpex lacteus PCV1) and schizophyllum commune (schizophyllum commune SCF1) for preparing active dietary fibers by fermenting potato dregs, and a method for converting the potato dregs to synthesize the active dietary fibers through degradation of irpex lacteus PCV1 and schizophyllum commune SCF1. The Irpex lacteus PCV1 is the strain belonging to the polyporales, polyporaceae and the irpex lacteus genus; the Irpex lacteus PCV1 strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on July 6, 2016,and is assigned with the accession number of CGMCC NO.12529. The schizophyllum commune SCF1 is the strain belonging to the agaricales, schizophyllaceae and the schizophyllum; the schizophyllum commune SCF1 strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on July 6, 2016, and is assigned with the accession number of CGMCC NO.12530. The method comprises thefollowing steps: inoculating Irpex lacteus PCV1 into a liquid fermentation culture medium containing the potato dregs, brain and inorganic salt for fermentation culture, after culture is finished, inactivating the fermentation liquor, after inactivating, inoculating the schizophyllum commune SCF1 for fermentation culture, carrying out homogenization treatment on the fermentation final product, and thus the active dietary fiber product is obtained.

Description

technical field [0001] The present invention relates to new bacterial strains capable of fermenting potato dregs to prepare active dietary fiber——Irpexlacteus PCV1 and Schizophyllum commune SCF1, and a method for synthesizing active dietary fiber by using them to degrade and transform potato dregs. Background technique [0002] In 1972, Trowell et al. defined dietary fiber as: polysaccharide carbohydrates and lignin that are not digested and absorbed by the human body when measuring various nutrients in food. With the deepening of people's research on dietary fiber, its definition has been continuously improved. On November 2, 1999, at the annual meeting of the American Association of Cereal Chemists (AACC), dietary fiber was defined as: edible plant-based ingredients, carbohydrates that can resist digestion and absorption in the small intestine of the human body, and can be partially or fully fermented in the large intestine of the human body. The sum of compounds and simi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/02A23L33/22C12R1/645
Inventor 孙敏
Owner 孙敏
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