Eucommia ulmoides key fruit hull treating method beneficial to extraction of gutta-percha
A technology of Eucommia samara and treatment method, which is applied in the field of treatment of Eucommia samara shell, can solve the problems of long extraction time, poor penetration, and environmental pollution, and achieve excellent treatment effect, improved penetration, and excellent environmental benefits Effect
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Embodiment 1
[0014] (1) Preparation of the seed liquid of the white rot fungus: Inoculate the white rot bacteria of the white rot fungus on a newly prepared PDA plate with a diameter of 9 cm, activate the mycelia for 4 days to cover the plate, and inoculate one plate of fungus The silk was inoculated into a shaker flask equipped with 400 mL of PDA liquid medium, and cultured on a shaker at 27° C. for 76 hours to obtain a seed liquid of R. albicans.
[0015] (2) Inoculation and cultivation of samara shell of Eucommia ulmoides: Soak samara shell of Eucommia ulmoides in water for 15 hours and make the water content of the shell 65%. The inoculated fruit shells are piled up for fermentation, and the ambient temperature is kept at 27°C and the humidity is 82% to ferment for 14 days. During the fermentation period, the fungus albicans occurs, and the shell material is filled with white hyphae until the mycelium slowly dissolves.
[0016] (3) Treatment after fermentation: After the fermentation i...
Embodiment 2
[0019] (1) Preparation of the seed solution of the white rot fungus: Inoculate the white rot bacteria of the white rot fungus on a newly prepared PDA plate with a diameter of 9 cm, activate the mycelia for 5 days to cover the plate, and inoculate one plate of bacteria The silk was inoculated into a shaker flask equipped with 400 mL of PDA liquid medium, and cultured on a shaker at 28° C. for 62 hours to obtain a seed liquid of R. albicans.
[0020] (2) Inoculation and cultivation of samara shell of Eucommia ulmoides: Soak samara shell of Eucommia ulmoides in water for 11 hours and make the water content of the samara 73%, inoculate the seed liquid of eucommia samara according to 0.99% of the mass of samara eucommia husk, and The inoculated husks were piled up for fermentation, and kept at an ambient temperature of 29°C and a humidity of 90% for 11 days of fermentation. During the fermentation period, the fungus albicans occurred, and the husk material was filled with white myce...
Embodiment 3
[0024] (1) Preparation of the seed liquid of the white rot fungus: Inoculate the white rot bacteria of the white rot fungus on a newly prepared PDA plate with a diameter of 9 cm, activate the mycelia for 6 days to cover the plate, and inoculate one plate of bacteria The silk was inoculated into a shaker flask equipped with 400 mL of PDDA liquid medium, and cultured on a shaker at 29° C. for 88 hours to obtain a seed liquid of R. albicans.
[0025] (2) Inoculation and cultivation of the samara shell of Eucommia ulmoides: Soak the samara shell of Eucommia ulmoides in water for 19 hours and make the water content of the samara 69%, inoculate the seed liquid of eucommia samara according to 1.17% of the mass of the samara shell of Eucommia ulmoides, and The inoculated fruit shells are piled up for fermentation, and the ambient temperature is kept at 28°C and the humidity is 95% to ferment for 12 days. During the fermentation period, the fungus albicans occurs, and the shell material...
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