Preparation method and application of microbial straw degradation microbial agent
A technology of degrading bacteria and microorganisms, which is applied in the field of preparation of microbial straw degrading bacterial agents, can solve the problems of short returning time, shortening the time of straw returning to the field, and the limitation of the process of straw returning to the field naturally, so as to improve the soil micro-environment As well as the ecological community structure, solving the technical problems of straw returning to the field and soil improvement, and the effect of high-efficiency degradation
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Embodiment 1
[0034] The preparation method of microbial straw degrading bacteria agent comprises the following steps:
[0035]1) Prepare strains Irpex lacteus, Aspergillus sydowii, Penicillium citrinum, Schizophyllum commune;
[0036] 2) Take the strains prepared in step 1) and perform activation, cultivation, and fermentation respectively:
[0037] a) Activation of strains: respectively activate the strains of Irpex lacteus, Aspergillus sydowii, Penicillium citrinum, and Schizophyllum commune on a slant, the activation temperature is 37°C, and the activation time is 24h, and then purified on a plate, the purification temperature is 37°C, and the purification The time is 24h, and the corresponding strain plate is obtained;
[0038] b) Strain cultivation: Take a ring of Irpex lacteus, Aspergillus sydowii, Penicillium citrinum, and Schizophyllum commune strains, respectively, and insert them into the Erlenmeyer flasks containing the seed medium, and culture them on a shaking table. The cult...
Embodiment 2
[0042] The preparation method of microbial straw degrading bacteria agent comprises the following steps:
[0043] 1) Prepare strains Irpex lacteus, Aspergillus sydowii, Penicillium citrinum, Schizophyllum commune;
[0044] 2) Take the strains prepared in step 1) and perform activation, cultivation, and fermentation respectively:
[0045] a) Activation of strains: respectively activate the strains of Irpex lacteus, Aspergillus sydowii, Penicillium citrinum, and Schizophyllum commune on a slant, the activation temperature is 37°C, and the activation time is 24h, and then purified on a plate, the purification temperature is 37°C, and the purification The time is 24h, and the corresponding strain plate is obtained;
[0046] b) Strain cultivation: Take a ring of Irpex lacteus, Aspergillus sydowii, Penicillium citrinum, and Schizophyllum commune strains, respectively, and insert them into the Erlenmeyer flasks containing the seed medium, and culture them on a shaking table. The cul...
Embodiment 3
[0050] The preparation method of microbial straw degrading bacteria agent comprises the following steps:
[0051] 1) Prepare strains Irpex lacteus, Aspergillus sydowii, Penicillium citrinum, Schizophyllum commune;
[0052] 2) Take the strains prepared in step 1) and perform activation, cultivation, and fermentation respectively:
[0053] a) Activation of strains: respectively activate the strains of Irpex lacteus, Aspergillus sydowii, Penicillium citrinum, and Schizophyllum commune on a slant, the activation temperature is 37°C, and the activation time is 24h, and then purified on a plate, the purification temperature is 37°C, and the purification The time is 24h, and the corresponding strain plate is obtained;
[0054] b) Strain cultivation: Take a ring of Irpex lacteus, Aspergillus sydowii, Penicillium citrinum, and Schizophyllum commune strains, respectively, and insert them into the Erlenmeyer flasks containing the seed medium, and culture them on a shaking table. The cul...
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