Application of irpex lacteus in preparing anti-hypoxia medicaments
A technology of Racula albicans and hypoxia resistance, which is applied in the field of medicine, can solve the problems of different types of Racula albicans, achieve the effects of small toxic and side effects, simple preparation method, and low cost
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Embodiment 1
[0014] Embodiment 1: the preparation of the extract of Racula albicans
[0015] The fermented liquid was obtained by fermenting the fungus albicans, centrifuged at 4000 rpm for 10 minutes, the supernatant was discarded, and the precipitate was freeze-dried to obtain the fungus powder, which was then extracted with deionized water for 2 The second time, according to the liquid-to-material ratio of 1:40, the temperature is 90 ° C, the first time is 3 hours, the second time is 1 hour, the two water extracts are combined, and the filtrate is obtained with a 200 mesh filter cloth, and the filtrate is concentrated. Add ethanol until the alcohol concentration is 80%, stir overnight, filter and take the precipitate, and freeze-dry the precipitate to obtain the extract of R. albicans.
Embodiment 2
[0017] The fermented liquid was obtained by fermenting the fungus albicans, centrifuged at 4500 rpm for 8 minutes, the supernatant was discarded, and the precipitate was freeze-dried to obtain the fungus powder of the fungus. The material ratio is 1:40, the temperature is 80°C, the first time is 3 hours, the second time is 1 hour, the two water extracts are combined, and the filtrate is obtained with a 200-mesh filter cloth, the filtrate is concentrated, and then ethanol is added to ethanol. The concentration is 80%, stir overnight, filter and take the precipitate, and freeze-dry the precipitate to obtain the extract of R. albicans.
Embodiment 3
[0019] Get the fermented liquid from the fungus A. albicans through a fermenter, centrifuge it at 5000 rpm for 5 minutes, discard the supernatant, and freeze-dry the precipitate to obtain the powder of the fungus, then extract the powder twice with water, press the liquid The material ratio is 1:40, the temperature is 70°C, the first time is 3 hours, the second time is 1 hour, the two water extracts are combined, and the filtrate is obtained with a 200-mesh filter cloth, the filtrate is concentrated, and then ethanol is added to ethanol. The concentration is 80%, stir overnight, filter and take the precipitate, and freeze-dry the precipitate to obtain the extract of R. albicans.
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