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Irpex lacteus and application thereof

A technology of rhizoctonia albicans and strains, which is applied in the field of environmental microorganisms, can solve the problems of high requirements on culture conditions, decreased degradation effect, low optimal concentration, etc., and achieves the effects of protecting the ecological environment, strong degradation ability and fast growth rate.

Active Publication Date: 2017-11-24
SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] It can be seen from the above table that the current research on the degradation of tetracycline antibiotics is mainly based on laboratory liquid culture, which requires high culture conditions. Most culture temperatures are above 30°C, which is difficult to achieve in practical applications.
However, the degradation experiment of Pleurotus rugosa has good results, but it has the disadvantages of low degradation concentration and long degradation time.
The optimal concentration for degradation by bacteria other than yeast is also relatively low, below 100mg / L, when the concentration increases, its degradation effect will decrease

Method used

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  • Irpex lacteus and application thereof
  • Irpex lacteus and application thereof
  • Irpex lacteus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Albicans albicans is isolated from rotting wood in primitive forests. The isolation and cultivation methods of the fungus are as follows:

[0029] 1. Medium preparation:

[0030] (1) Strain isolation and preservation medium (solid, 1L): 20g of malt extract powder, 17g of agar, add water to 1L, and the pH value is natural;

[0031] (2) Solid medium (solid, 1Kg): birch shaving flakes and rice straw are mixed in a volume ratio of 1:2, the dry weight after mixing is 1Kg, and water is added to 1500ml; among them, the diameter of the wood chip is 0.5-1cm, and the length of the straw is 1- 2cm;

[0032] Both of the above two media were sterilized in a pressure cooker at 121° C. for 20-30 minutes.

[0033] 2. Strain activation, solid culture preparation:

[0034] Activation of strains: pick the strain of R. albicans and inoculate it in a 60mm petri dish added with isolation and preservation medium, and culture it at 28-30°C for 4-5 days.

[0035] Cut the above-mentioned culti...

Embodiment 2

[0038] 1) Cultivation of strains: Pick up the mycelia of Irpex lacteus IFP00606 with an inoculation needle and inoculate them into the plate of the culture medium for the separation and preservation of the above strains, and cultivate them in an incubator at a temperature of 28°C for 4 days. Loose, snowy white, cottony. Cut it evenly into small pieces of hyphae with a sterilized hole puncher with a diameter of 6mm, inoculate it into a solid culture substrate, inoculate 5-10% (V / V), cultivate it at 28°C for 15 days, and wait for the bacteria to grow. The silk is covered with matrix, which is ready for fungicide.

[0039] The configuration of the solid culture substrate is solid matter and liquid matter, and 15 grams of liquid matter is added for every 10 grams of dry solid matter; wherein, the solid matter is a mixture of birch shavings and straw, and the liquid matter is starch 0.5wt%, urea 0.3wt%, Copper sulfate 1wt%, the balance is water; the birch shavings and rice straw a...

Embodiment 3

[0041] Degradation of Oxytetracycline Residues by Degrading Bacteria by Solid State Fermentation

[0042] (1) configuration oxytetracycline content is 245ppm water 5L;

[0043] (2) Take about 0.5kg of the obtained solid-state fermentation degrading bacteria agent, add it to 5L of water containing oxytetracycline, simulate outdoor conditions for treatment, use a surf pump to circulate the water, and treat at room temperature (25-30°C) for 5 days . And the uninoculated and sterilized rice straw was used as the control.

[0044] (3) Samples were taken every 24 hours during the degradation process, and 2ml of water samples were taken and placed in a 5mL plastic centrifuge tube for centrifugation (model: KDC-40) at a rotation speed of 4000rpm, and the supernatant was taken out.

[0045] (4) Perform gas chromatographic analysis on the supernatant collected by centrifugation for each sampling. Calculate the degradation rate: degradation rate (%)=(A-B) / A×100, wherein A is the oxyte...

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Abstract

The invention belongs to the field of environmental microorganism, and relates to irpex lacteus and application thereof. The irpex lacteus is a strain IFP00606 and preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation date is July 13, 2017, and the preservation number is CGMCC No.14352. A degrading bacterium produced by the technical scheme can effectively repair tetracycline antibiotic residue polluted water, reduce the harm on a human body by the antibiotic residue and protect the ecological environment.

Description

technical field [0001] The invention belongs to the field of environmental microbes, and relates to a white sac bacterium and an application thereof. Background technique [0002] As antibacterial or bactericidal drugs, antibiotics have been widely used in the treatment of human diseases, livestock and poultry and aquaculture and other fields, mainly including tetracyclines, sulfonamides, β-lactams, fluoroquinolones and macrolides Wait. my country is a big country in the production and use of antibiotics, with an annual production of 210,000 tons of antibiotics and a usage of 189,000 tons, of which veterinary antibiotics account for more than half of the usage. Studies have found that after organisms ingest a large amount of antibiotics, in addition to being partially metabolized by the body, 40% to 90% are excreted in the form of original drugs or primary metabolites with feces and urine, and finally enter the soil through fertilization and other methods. environment or i...

Claims

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Application Information

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IPC IPC(8): C12N1/14C02F3/34C12R1/645
CPCC02F3/34C02F2103/343C12N1/14C12N1/145C12R2001/645
Inventor 魏玉莲袁海生李通
Owner SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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