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A strain of albicans and its application

A technology of white sac rake tooth fungus and mycelium, applied in the direction of application, fungi, chemicals for biological control, etc., to achieve the effect of promoting root growth of crops, increasing yield, and promoting flowering

Active Publication Date: 2019-09-20
SHANDONG PENGBO BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on R. albicans mainly focuses on its hypoxia resistance (CN104138395A), anti-fatigue effect (CN104138396A) and lignin degradation effect (CN105754881A); , Improving disease resistance and fertilizer utilization has not been reported

Method used

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  • A strain of albicans and its application
  • A strain of albicans and its application
  • A strain of albicans and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Isolation and identification of bacterial strains

[0027] 1. Isolation and screening of strains:

[0028] (1) Isolation of strains

[0029] Take fresh Daxinganling wild blueberry root samples and rinse them with running water, dry them with filter paper, cut them into appropriate sizes, wash them with sterile water for 3 times, and put them in 10% H 2 o 2 Sterilize in medium for 10 minutes, rinse 3 times with sterile water, soak in 6% sodium hypochlorite solution for 2 minutes, then rinse 3 to 5 times with sterile water, put it on a sterile filter paper, and blot the surface moisture. Use sterile scissors to cut into root segments of about 1 cm, and inoculate them on the PDA medium after high-temperature and high-pressure sterilization at 121° C. for 30 minutes. After being placed in an incubator at 23°C for 3-10 days, hyphae can be seen growing from the cut edge of the sample, which is repeatedly separated and purified on the plate until each plate conta...

Embodiment 2

[0044] Embodiment 2: the preparation of plant inducer

[0045] The specific preparation method is as follows:

[0046] 1. Fermentation culture:

[0047]The bacterial classification of Irpex lacteus PR2 isolated and preserved in Example 1 preserved on the slant of the test tube at 4°C was placed on the PDA medium, cultivated at 25°C for one week, and the agar was dug into pieces and inoculated in 50mL PDA medium. The 250mL Erlenmeyer flask of culture medium was cultivated at 25°C for 3 days on a rotary shaker at 180r / min as seeds, and inoculated into a 500mL Erlenmeyer flask containing 150mL fermentation medium with 10% of the amount, cultivated for 5 days under the same conditions, and terminated Ferment to obtain seed liquid.

[0048] PDA medium formula: potato 200g, glucose 20g, agar 20g, water 1000ml.

[0049] Fermentation medium formula: 1.0L of potato extract, 1.0g of yeast extract, 3.0g of peptone, 15.0g of glucose, and 17.0g of agar.

[0050] Preparation of potato e...

Embodiment 3

[0053] Embodiment 3: the application of plant inducer in potato growth promotion and production increase

[0054] 1. Test method:

[0055] The potato sowing date is April 18-19, 2015, the sowing density is 3300-3500 plants / mu, the ridge spacing is 55cm, and the plant spacing is 35cm. During the implementation process, the farming operations during the potato growing season are the same as field planting.

[0056] Treatment 1: conventional treatment, that is, when sowing, no endophyte extract (that is, the plant inducer prepared in Example 2) was added to spray the ditch; during the expansion period, amino acid water-soluble fertilizer was drip-irrigated with water.

[0057] Treatment 2: when sowing, endophyte extract (i.e. the plant inducer prepared in Example 2) 10ml / mu spray ditch; in the expansion stage, amino acid water-soluble fertilizer, drip irrigation with water.

[0058] Process 3: when sowing, endophyte extract (i.e. the plant inducer prepared in Example 2) 10ml / mu...

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Abstract

The invention discloses a strain of Irpex lacteus, named Irpex lacteus (Irpex lacteus) PR2, which has been preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, referred to as CGMCC, and the address is: Beichen, Chaoyang District, Beijing No. 3, No. 1 Yard, West Road, Institute of Microbiology, Chinese Academy of Sciences, strain preservation number is CGMCC NO.13190. The present invention is the first time to isolate and purify a kind of R. albicans from the root of wild blueberry in Daxing'an Mountains. It uses liquid aerobic fermentation to obtain a large number of hyphae of R. albicans, uses ethanol as the extraction solvent, and obtains it through ultrasonic extraction. Natural substances that grow crop roots, improve stress resistance, promote flowering, increase yield and crop quality, are conducive to the further development of natural compounds with ultra-high life-promoting activities; and no three wastes are generated during the entire production process, which is cost-effective and meets the requirements of Social needs for environmental protection and food safety production.

Description

technical field [0001] The invention relates to a strain of albicans rake tooth fungus and its application, and belongs to the field of biotechnology. Background technique [0002] With the increase of population and the decrease of arable land, the contradiction between my country's food security, resource consumption and environmental protection has become increasingly acute. Fertilizer plays an important role in agricultural production, but there are some unreasonable factors in the process of use, which lead to the failure of the production performance of fertilizer to be effectively exerted, resulting in the decline of soil fertility, the decrease of fertilizer utilization rate, the increase of agricultural production cost, and the decrease of benefit. It leads to the decline of the quality of agricultural products, pollutes the ecological environment, accelerates the decline of soil quality, and directly affects the continuous increase of grain production, the improvem...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P1/02A01N63/04A01P21/00C12R1/645
CPCA01N63/30C12P1/02C12N1/145C12R2001/645
Inventor 代丽丽
Owner SHANDONG PENGBO BIOTECHNOLOGY CO LTD
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