33 results about "Mycology culture" patented technology

The invention provides biologically active compositions comprising oligosaccharides, and which are produced by growing a fungal culture, and are at least partially purified for use. The compositions of the invention have antibacterial, antifungal and nematicidal activity, and are thus useful to reduce the impact of such pathogens on growing plants, to reduce the occurrence of such pathogens on surfaces and in substances, and to treat infections caused by such pathogens in animals and humans. The invention also provides methods for producing such compositions from certain fungal cultures.

The invention discloses an extract for marine fungi culture, a preparation method thereof and application thereof. The extract is an oily matter prepared by the following steps: after seed culture and fermentation culture of marine fungi CCTCC M 208095, collection of culture fermentation broth, extraction of the fermentation broth by ethyl acetate and concentration and drying of the residual matter. The extract has the advantages of resisting pathogenic bacteria and oral cancer cells KB and inhibiting acetylcholinesterase, topoisomerase I, and a drug-resistant strain KBv200 of the extract, and can be used for preparing antibacterial and antineoplastic medicines for treating senile dementia.

Three new alkaloids, Hirsutellones A, B, and C, are produced in cultures of fungus such as Hirsutella nivea strain BCC 2594 and Trichoderma sp. strain BCC 7579. The Hirsutellones exhibited potent growth inhibitory activity against Mycobacterium tuberculosis (H37Ra strain) with an MIC value of 0.78 μg / ml, while showing weak or no cytotoxicity to mammalian cells. Therefore, Hirsutellones and pharmaceutical compositions containing Hirsutellones may be useful for the treatment of tuberculosis. Also described herein are methods of isolating the Hirsutellones.

A method of treating drain systems containing organic matter and providing a biopesticide for killing insects is provided. The method comprises introducing into a drain system a bacterial culture, such as Bacillus sp or Pseudomonas sp, which metabolizes the organic matter, and a biocidal amount of an entomopathogenic fungal culture, such as Metarhizium, which kills the insects such as cockroaches or other soft-bodied insects. The drain system can be a residential or commercial drain system. The bacterial culture and the fugal culture can be maintained separately and then mixed for the treating of the drain, or they can be maintained together prior to introducing them into the drain. The microorganism cultures are dispersed separately or together into the drain. The cultures can be dispersed as a spray, a powder, a liquid, or a foam.

In one embodiment, a testing device includes a substrate and a plurality of spatially distinct fungal cultures disposed on the substrate.

Methods are provided for cultivating fungi for scaled-up production of microbe-based products. Specifically, cultures of fungi, such as, e.g., Pisolithus tinctorius, are grown by aerobic submerged fermentation in liquid medium containing a particulate anchoring carrier to increase mycelial biomass.

The invention relates to a fungus colony PCR method and a pathogenic fungus identification method. The fungus colony PCR method aims to achieve the high positive rate of amplification, and the pathogenic fungus identification method aims to achieve short detection time and reliable results. The fungus colony PCR method comprises the following steps of: inoculating fungus cultures onto a culture medium for culture; taking fungus colonies which begin to appear on the culture medium as experimentally available colonies; and preparing a DNA template for colony PCR amplification. The pathogenic fungus identification method comprises the following steps of: inoculating clinical sample cultures or pathogenic fungus cultures onto the culture medium for culture; taking the colonies which begin to appear on the culture medium as the experimentally available colonies; preparing the DNA template for the PCR amplification; and detecting amplification products. The fungus colony PCR method and the pathogenic fungus identification method have the advantages of improving the positive rate of the fungus colony PCR amplification, solving the problems of complex steps, wall-breakage difficulty, longtime and the like in fungus genomic DNA extraction, performing colony identification at the very beginning of the growth of pathogenic fungi, and achieving reliable results and identification time remarkably shorter than conventional fungus phenotype identification time.

The present invention relates to a kind of ethyl acetate extract effective part oil of the culture endophytic fungus of South China Sea mangrove and its preparation method and this extract is used for preparing and killing cotton bollworm [Heliothis armigera (Hühner)] and fish parasite Pesticide application of Sinergasilus spp.