Manufacturing method of dry preserved specimen of hyphomycetes culture

Abstract

The invention discloses a manufacturing method of a dry preserved specimen of hyphomycetes culture. The manufacturing method comprises the following steps: adding a prepared agar culture medium into a culture dish to manufacture an agar culture plate, paving wet filter paper with a plurality of holes on the surface of the agar culture plate, inoculating hyphomycetes strains on the centers of the filter paper holes, sealing the culture disc, placing the culture disc at a room temperature under the nature lights to obtain hyphomycetes culture; taking out the filter paper, placing the filter paper on another culture disc, fumigating the filter paper in a formaldehyde fumigating barrel for 6 to 8 days, and then naturally drying the filter paper in the air in a place with good ventilation so as to obtain the dry preserved specimen of hyphomycetes culture. The provided method has the advantages of simple operation and mild culture conditions; the spore yield of hyphomycetes is prominently increased, the method is effective for most hyphomycetes, conidia are evenly distributed on the filter paper, the observation on the conidia is convenient, the phenotype of conidia can be observed conveniently, moreover, the drying time of species is greatly shortened, and the efficiency and quality of species manufacturing are both improved.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a technology for preparing dried specimens of hyphosporium fungus culture. Background technique [0002] For a long time, the classification and identification of hyphosporium fungi have mainly been based on the morphological characteristics of the fungus, and the characteristics of conidia (shape, size, number of septa, surface decoration and appendages, etc.) The appearance of continuous sporulation (sporulation phenotype) is also an important basis for the identification of hyphosporium species. Dried specimens of cultures are one of the important ways for long-term preservation of hyphomycetes. [0003] Hyphosporium fungi tend to fail to produce conidia or hardly produce conidia during artificial culture. At present, the main methods for promoting hyphosporium sporulation include colony scratching method, ultraviolet light irradiation method, freezing stimulation method, d...

AI Technical Summary

Problems solved by technology

At present, the main methods for promoting hyphosporium sporulation include colony scratching method, ultraviolet light irradiation method, freezing stimulation method, dark / light alternate culture method, etc., but these methods all have the following disadvantages: (1) the ability to promote sporulation The effect is not obvious, the amount of sporulation is small, and the sporulation phenotype is difficult to observe; (2) it is difficult to accurately grasp the treatment time, and improper treatment time will inhibit the sporulation or death of the hyphosporium fungus; (3) the culture cycle is long, often after a long period of time (4) The characteristics of conidia produced are unstable, and there are large differences in the characteristics of conidia produced under different culture conditions

But this method has the following disadvantages: (1) The growth characteristics of different types of hyphomycetes are quite different, and the types of culture media required are also different. (2) In the process of specimen preparation, in addition to culturing hyphomycetes, it is still necessary to prepare water agar medium, sterilize, prepare culture plates, and dig out fungal colonies, etc. , the production steps are loaded down with trivial details; (3) the culture medium and the water agar culture plate that are used for hyphomycetes culture all contain a large amount of water, cause specimen to take a long time and just can fully dry, fully dry needs 15~20 days when the weather is fine, When the weather is bad, it takes longer to dry and the specimens tend to become smelly and lose their preservation value

Images