78 results about "Androstenedione serum" patented technology

Fermentation of phytosterol compositions to produce androstenedione (androstene-3,17-dione) and subsequently androstenedione (androstene-1,4-diene,3,17-dione) A method comprising: propagating a microbial culture of the genus Mycobacterium in a first nutrient medium; placing the microbial culture and the phytosterol composition in a bioreactor for a sufficient time to convert the composition substantially to androgen enedione ("AD solution"); propagation of a fungal culture of Fusarium in a second nutrient medium; infusion of one or more of 1) Fusarium species or 2) fungal culture medium into the organism In the reactor, react for sufficient time to convert the AD solution to androsdienedione.

Methods are provided for detecting the amount of one or more CAH panel analytes (i.e., pregnenolone, 17-OH pregnenolone, progesterone, 17-OH progesterone, dehydroepiandrosterone (DHEA), androstenedione, testosterone, deoxycorticosterone, 11-deoxycortisol, and cortisol) in a sample by mass spectrometry. The methods generally involve ionizing one or more CAH panel analytes in a sample and quantifying the generated ions to determine the amount of one or more CAH panel analytes in the sample. In methods where amounts of multiple CAH panel analytes are detected, the amounts of multiple analytes are detected in the same sample injection.

Methods for treating or reducing the likelihood of acquiring estrogen-related (e.g. estrogen-exacerbated) diseases including endometriosis include administering to a patient a selective estrogen receptor modulator (SERM), in combination with inhibiting ovarian secretions, e.g., by administering an LHRH agonist or antagonist. In some embodiments, a precursor of sex steroids, said precursor being selected from the group consisting of dehydroepiandrosterone (DHEA), dehydroepiandrosterone sulfate (DHEA-S), androst-5-ene-3β,17β-diol (5-diol), and androstenedione or a compound transformed into one of these, is also administered

The invention discloses a microbial strain for preparing androstenedione. The collection number of the microbial strain is CCTCC NO:M2012522. The preparation of androstenedione by the utilization of the strain comprises the following steps: A, primary seeding tank fermentation: inoculation amount of the strain is 0.5-1.5%, and cultivation is carried out at 160-200rpm and at 0.03-0.07MPa for 30-42h; B, secondary seeding tank fermentation: inoculation amount of the strain is 8-12%, and cultivation is carried out under the same conditions for 20-30h; C, fermentation tank fermentation for conversion to generate androstenedione: inoculation amount of the strain is 10-14%, and cultivation is carried out under the conditions of 27-35 DEG C and 0.03-0.07MPa; and D, termination of conversion: sterol is lower than 0.5%, pH is 8.9-9.0, discharging, heating to 90-100 DEG C and keeping for 30-50min, cooling to 30-50 DEG C, stopping stirring and standing for 4-6h. The invention provides a high-efficiency fermentation strain of mycobacterium. Meanwhile, a surfactant, soya-bean oil and a broth are used to form a two-way system. The fermentation technology is improved, and feeding amount of raw materials and conversion rate of microorganisms are greatly raised.

The invention belongs to the technical field of bio-catalysis, and in particular relates to a method for improving regeneration of mycobacterium coenzyme and for simultaneously promoting production ofandrostenedione. According to the method, sterol C27 site monooxygenase Cyp125 in a phytosterol metabolic pathway is introduced into steroid transformation microorganism, so as to improve androstenedione conversion efficiency of recombinant microorganism in a fermentation system; and therefore, a novel method for solving a problem of slow metabolism of mycelial phytosterol is offered. Through over-expression of the Cyp125, specific enzyme activity of the mycobacterium sterol C27 site monooxygenase is improved by 22.2%; regeneration of intracellular NAD+ is improved, and an intracellular NAD+/NADH ratio is improved by 131%; and meanwhile, it is unexpectedly discovered that a bacterium biomass amount can be improved by 18.7% through the over-expression of the enzyme, and finally, effects ofshortening a conversion cycle by 24h and improving an AD(D) yield by 10.0% can be achieved.

The invention relates to a method for simultaneously detecting five kinds of steroid hormones in serum. The five kinds of steroid hormones are respectively testosterone, androstenedione, 11-deoxycortisol, cortisol and cortisone. The method for simultaneously detecting the five kinds of steroid hormones in the serum comprises the following steps: sample pretreatment: adding an acetonitrile solution of an internal standard substance in a serum sample for carrying out protein precipitation, then adding tert-butyl methyl ether for extracting, blow-drying supernatant, adding a combination solution, and taking the supernatant, thus obtaining a to-be-detected sample; enrichment, separation and detection: carrying out enrichment, separation and detection on the to-be-detected sample by adopting a two-dimensional liquid chromatography-tandem quadrupole mass spectrometer. According to the method for simultaneously detecting the five kinds of steroid hormones in the serum, disclosed by the invention, the method is adopted for simultaneously detecting five kinds of compounds of which the concentration ranges are inconsistent in the serum, and the time for simultaneously detecting the five kinds of the compounds is about 8.5 to 12.0 minutes; the method for simultaneously detecting the five kinds of steroid hormones in the serum is low in cost, high in flux, high in precision degree and strong in specificity.

The invention provides a method for preparing androstenedione using a double liquid phase fermentation system by fermenting Mycobacterium sp.DE6823, and aseptic distilled water is selected as a fermentation medium water phase, and rice oil, sunflower oil, soybean oil and peanut oil can be selected as an oil phase, after fermentation conversion, a solution containing androstenedione is obtained, and after condensation, decolouring, filtering, condensation, recrystallization and vacuum drying and other operations, and androstenedione whose purity is above 99% is obtained. Different oil phases can be selected, and the conversion rate of the plant sterol is in the range of 59%-85%. The invention solves the problems that the raw materials of the present androstenedione production technology in our country are limited by season and region, thereby effectively reducing destroy to ecological environment; the invention has the characteristics of simple operation, small environmental pollution, high conversion rate, low production cost, etc., and is suitable for industrialized production.

The invention provides a method for screening a I type 17 beta-hydroxysteroid dehydrogenase inhibitor through an immobilized enzyme, and belongs to the technical field of enzymology and enzyme engineering. I type 17 beta-hydroxysteroid dehydrogenase (17 beta-HSD1) has the important function in treating hormone-dependent diseases. At present, a substrate radiolabelling method is mainly adopted in studying the activity of 17 beta-HSD1, due to the fact that the free enzyme of 17 beta-HSD1 is likely to be inactivated, 17 beta-HSD1 is hard to prepare, a fresh placenta is needed for preparing an enzyme source in a new medicine screening experiment of every time, raw materials are hard to obtain, the price is high, and difficulty is brought to the screening work of new medicine. According to the method, an amino-modified silicon ball is adopted as a carrier, a glutaraldehyde crosslinking method is utilized, 17 beta-HSD1 extracted from the placenta is fixed, an external immobilization 17 beta-HSD1 enzyme model is built, androstenedione is adopted as a substrate, a high performance liquid chromatography method is used for detecting products, and the potential 17 beta-HSD1 inhibitor is screened. According to the method, instability of the free enzyme is overcome, operation is easy, the manufacturing cost is low, and repeated using is achieved.

The invention discloses a method for simultaneously determining 15 forbidden androgens in cosmetics. The method comprises the following steps: (1) preprocessing a sample: extracting, purifying and concentrating the sample; (2) analyzing and determining the preprocessed sample by adopting an ultrahigh-efficient liquid chromatography-mass spectrometry/mass spectrometry. The method can simultaneously determine 15 forbidden androgens including adrenosterone, trenbolone, boldenone, fluoxymesterone, oxandrolone, androstenedione, nandrolone, metandienone, testosterone, dehydroepiandrosterone, clostebol, androstanolone, mestanolone, chlorotestosterone acetate and testosterone propionate. The method for simultaneously determining the 15 forbidden androgens in the cosmetics is accurate, rapid and sensitive. According to the method, effective scientific evidence and technical support can be provided for the inspection work of cosmetics and the quality control of the daily production.

The invention belongs to the technical field of biological catalyst, and particularly relates to a mycobacteria genetically engineered bacterium with cofactor regeneration function and its applicationin fermentation of two liquid phases. Through a NADH oxidizing system in the cell, the genetically engineered bacterium for transforming plant sterol biology is structured and transformed for the NADH oxidizing system in the cell, thus the problem of low transforming efficiency of androstenedione in the oil water two-liquid-phase fermenting system is solved; the genetically engineered bacterium also provides possibility for the wide application of the oil water two-liquid-phase fermenting system in industrial production of the androstenedione. The genetically engineered bacterium MNR M3N2 structured by the invention can improve the AD (D) output by 1.4-2.9 times during the preparation process of androstenedione by degrading the phytosterol through the oil water two-liquid-phase fermentingsystem fermenting method.

The invention relates to a method recombining Yarrowia lipolytica to efficiently and biologically convert androstenedione so as to produce testosterone and belongs to the field of genetic engineering. The method is characterized in that 17beta-hydroxyl steroid dehydrogenase from human and glucose dehydrogenase from saccharomyces cerevisiae are respectively cloned and excessively co-expressed in Y.lipolytica Polh, and the androstenedione is efficiently converted in the Yarrowia lipolytica to produce the testosterone for the first time at home and abroad by building an NADPH coenzyme circulation system. The enzyme activity determination and intracellular coenzyme level researches of the built genetic-engineering strains show that the recombinant strains can continuously and effectively convert the androstenedione to produce the testosterone. When the conversion temperature is 37 DEG C, conversion pH is 7.5, substrate cosolvent is methylated-beta-cyclodextrin, biomass is 200g/L and substrate concentration is 5g/L, 15g/L androstenedione is converted into 14.3g/L testosterone after three material-supplementing whole-cell conversions, the currently-reported highest level of testosterone production using microorganism conversion is achieved, and a foundation is provided for industrial testosterone production using microorganisms.

The invention discloses a method for preparing 9alpha-hydroxide-androstenedione by utilizing microbial conversion. The method comprises the steps of: 1) by taking rhodococcus erythropolis as a seed lot, inoculating rhodococcus erythropolis in a seed culture medium, and culturing to obtain seeds; 2) inoculating the seeds into a conversion culture medium, and culturing to obtain fermentation liquor; 3) adding an androstenedione solution into the fermentation liquor, continuing culturing to obtain conversion liquor, and detecting the content of 9alpha-hydroxide-androstenedione in the conversion liquor; and 4) separating and extracting the 9alpha-hydroxide-androstenedione in the conversion liquor. The method has the advantages of being high-efficiency and specific, environment-friendly, high in added materail concentration, high in conversion rate, product purity and yield, low in cost and the like, is simple in overall preparation process, and economic and practical, has wide application prospects, and has very important significance on the application for extending the steroid raw materail drug androstenedione.

The invention discloses Ketosteroid C27-monooxygenase derived from mycobacterium neoaurum and application of the ketosteroid C27-monooxygenase, and belongs to the technical field of gene engineering and enzyme engineering. By means of gene knockout and expression strengthening methods, three isoenzymes of a key enzyme SMO in the sterol side chain degradation process are screened out from the mycobacterium neoaurum; the isoenzymes are intensively expressed in mycobacterium neoaurum of high-yielding androstenedione (ADD) separately, the ADD yield is obviously increased, and the effect of SMO2 is most obvious. By overexpressing SMO2, the final yield of ADD is increased to 7.3 g/L from 5.2 g/L. The Ketosteroid C27-monooxygenase derived from the mycobacterium neoaurum and the application of the ketosteroid C27-monooxygenase provide beneficial guidance for industrialization of increasing the ADD yield through a microbiological fermentation method.

The invention discloses a method for extracting androstenedione in a bi-liquid phases system. which is characterized in that a broth is pretreated, and is extracted by methanol and is performed with vacuum concentration until no methanol is generated, and then filtered to obtain a crude product I, washing the crystal by a mixing solvent to obtain a crude product II, and the crude product II is performed through twice methanol purification and dried to obtain the androstenedione pure product. The method has the advantages of good extraction effect and high product purity, methanol is taken as a main solvent, the cost is low, and the method is suitable for industrial production.

The invention discloses preparation methods of danazol and an intermediate thereof. The preparation method of danazol is prepared by the steps of taking androstenedione as a starting raw material, and carrying out 3-site enol etherification, 17-site carbonyl ethinylation, 3-site hydrolysis, 2-site methylidynel hydroxylation and oximation to obtain danazol. The 3-site enol etherification comprises firstly carrying out a reaction of androstenedione and triethyl orthoformate for 4-10 h in the presence of absolute ethyl alcohol and p-toluenesulfonic acid and at the temperature of 30-50 DEG C, then adding triethylamine at the temperature of 0-10 DEG C, and continuing to carry out a reaction for 0.2-1 h; the 17-site carbonyl ethinylation comprises firstly carrying out a reaction of a potassium hydroxide powder for 1-2 h in an acetylene airflow and at the temperature of 5-10 DEG C, and then carrying out a reaction with the 3-site enol etherified product for 2-4 h in the presence of tetrahydrofuran and a catalyst, at the temperature of 15-30 DEG C and in the acetylene airflow. The 3-site enol etherification is mild in reaction conditions and relatively high in yield, and the 17-site carbonyl ethynylation is relatively high in reaction yield and relatively short in time.

The invention provides a Chinese herbal compound for treating the pubertal polycystic ovary syndrome. The Chinese herbal compound is prepared from, by weight, 30-35 parts of radix astragali, 20-25 parts of American ginseng, 15-20 parts of Radix Salviae Miltiorrhizae,, 15-20 parts of hawthorn, 15-20 parts of poria cocos, 20-25 parts of bighead atractylodes rhizome and 15-20 parts of tangerine peel. The pubertal polycystic ovary syndrome (PCOS) is treated through the method of tonifying qi and spleens, reducing phlegm, activating blood and regulating constitution; compared with other traditional Chinese medicine research, the Chinese herbal compound can reduce levels of luteinizing hormone (LH), luteinizing hormone/follicle-stimulating hormone (LH/FSH), testosterone (T) and androstenedione (AND) in serum of patients and also can reduce the levels of total cholesterol (TC) and apolipoprotein B (APOB) in blood fat, and therefore the aims of regulating the menstrual cycle, improving hyperandrogenism and correcting dyslipidemia are achieved.

The invention provides a method for preparing androstenedione using a double liquid phase fermentation system by fermenting mycobacterium sp.DE6823, and aseptic distilled water is selected as a fermentation medium water phase, and rice oil, sunflower oil, soybean oil and peanut oil can be selected as an oil phase, after fermentation conversion, a solution containing androstenedione is obtained by employing a membrane separating method, and after condensation, decolouring, filtering, condensation, recrystallization and vacuum drying and other operations, androstenedione with purity of 99% or more is obtained. Different oil phases can be selected, and the conversion rate of the plant sterol is in the range of 59%-85%. The invention solves the problems that the raw materials of the present androstenedione production technology in our country are limited by season and region, thereby effectively reducing destroy to ecological environment; and the invention has the characteristics of simple operation, small environmental pollution, high conversion rate, low production cost, etc., and is suitable for industrialized production.

The invention belongs to the technical field of androstenedione production and specifically relates to a treating method for androstenedione fermented wastes and application of recovered materials. The method comprises the following steps: adding a demulsifying agent into the androstenedione fermented wastes at material liquor ratio of 1:(1-3); standing by, splitting phases and taking an upper phase under the condition at 20-60 DEG C; adding isometric demulsifying agent into a lower phase, and then standing by, splitting phases and taking the upper phase under the same temperature again; filtering the lower phase, thereby acquiring a filtrate; mixing the filtrate with the upper phase, thereby acquiring a mixed solution; crystallizing the mixed solution under the condition at 0-10 DEG C for two times, filtering and taking crystal, thereby acquiring sterol crystal; concentrating the filtrate and then precipitating the grease, thereby acquiring the grease. An organic solvent demulsification method and a low-temperature secondary crystallization technique are adopted by the invention for effectively recycling and reusing grease and sterol from the androstenedione fermented wastes, so that the production cost is saved and the pollution of the wastes is reduced.

The invention provides a method for detecting five androgens in serum. The method comprises the following steps of: mixing a serum sample to be detected with an internal standard solution, adopting amixed extraction solvent, obtaining detection liquid through liquid-liquid extraction pretreatment, and detecting the detection liquid by adopting an ultra-high performance liquid chromatography-tandem mass spectrum technology. The ultra-high performance liquid chromatography conditions are as follows: mobile phase A is 0.1% formic acid aqueous solution, and mobile phase B is 0.1% formic acid methanol solution. The mass spectrum conditions are as follows: a positive ion mode, namely a mass spectrum scanning mode of multi-reaction monitoring; and five androgens, which are dehydroepiandrosterone, testosterone, androstenedione, dihydrotestosterone, and androsterone. The method of the invention has the advantages that the detection time for simultaneously detecting five androgens is only fiveminutes, and the detection flux is large; the operation is simple and convenient, and the time consumption is less; and the method has the advantages of strong specificity, high sensitivity, high precision, low detection limit, wide detection range and wide practical application prospect.

The invention provides a high performance liquid chromatography method and application thereof. The method is characterized by carrying out component analysis of a steroid compound-containing mixture by a high performance liquid chromatography, wherein the high performance liquid chromatography adopts reversed high performance liquid chromatography and gradient elution, and mobile phases adopted by the gradient elution respectively are water and acetonitrile having the content of 95%. Through the high performance liquid chromatography method, the steroid compound-containing mixture (such as a mixture containing androstenedione and/or phytosterol) can be simultaneously analyzed. The high performance liquid chromatography method eliminates the interference factors, greatly shortens analysis time, has simple, reliable and accurate processes, and solves the existing problems of many operation steps, long analysis time and many interference factors. Therefore, the high performance liquid chromatography method provides an effective approach for steroid drug research and production process industrial production management.

The present invention provides a method for producing 11alpha-hydroxy-4-ene-3,17-androstanedione. The method comprises use of fungi to convert substrate 4-ene-3,17-androstenedione to the 11alpha hydroxy-4-ene-3,17-androstenedione, and includes the following steps: 1) collecting spores of a desired specie, and performing seed culture; 2) placing the seeds obtained in the step 1) in a conversion tank, and using a conversion culture medium to ferment and culture; 3) crushing the substrate, and beating with a 30 to 60% (v/v) aqueous ethanol solution to form a paste; 4) continuously adding the substrate of the step 3) in batches into the conversion culture medium in the conversion tank of the step 2) for 2 to 5 times, wherein in each time of adding, the amount of the added substrate is 1-3% (w/v) of the volume of the fermented liquid in the conversion tank, and the time for each time of adding is 4 to 5 hours. Compared with the prior art, the method provided by the invention improves the feeding amount of the substrate, the utilization rate and the conversion time greatly shorten the production cycle, and the method is an efficient and environmentally friendly production method.

The invention discloses a method for separating and preparing bisnorcholenaldehyde (BA) from crystallization mother liquor obtained after extraction of phytosterol fermentation liquor. The fermentation liquor obtained after fermentation of phytosterol through mycobacteria is further subjected to steps of extraction, crystallization and the like for extraction of androstenedione (4AD), a crystallization mother liquor condensed extract after the steps is completely dissolved by low-carbon alcohol, and then filtration is performed to obtain a filtrate containing BA. The filtrate containing BA is adsorbed by a small amount of resin, then is subjected to volatilization drying at a room temperature, and then is added into the top end of a chromatographic column filled with macroporous adsorption resin. Stepwise elution is performed by using mixed solutions of ethanol and water with different concentrations as eluents; and an eluent containing the BA is collected, and the eluent is subjected to evaporating concentration, then is completely dissolved by a mixed solution of methanol, acetonitrile and water, and then is crystallized at a low temperature, and the BA with the purity of 98.5% or more is obtained after filtration and crystal washing. The method is simple in process, the mother liquor can be purified and recycled, the cost is low, industrialization can be realized easily, the process is pollution-free and wastewater-free and is in line with environmental protection requirements, and an obtained product is in line with requirements of the pharmaceutical industry.

The invention belongs to the field of microbial pharmaceutics and provides a substrate treatment method for phytosterol side chain degradation reaction. The method is applied to phytosterol side chain degradation reaction for AD (androstenedione) production. Phytosterol and attapulgite are modified prior to feeding of phytosterol, and according to a substrate feeding quantity and an attapulgite addition amount, a mass ratio of the phytosterol to the attapulgite is controlled to be 1:0.1-5, and concentration of fed co-modified phytosterol and attapulgite is 1-40g/L. The substrate treatment method has advantages that dispersity of a substrate in an aqueous solution is effectively improved, the utilization rate and the reaction rate of the substrate are increased, and substrate and product inhibition is relieved to some extent to increase the conversion rate of products; in addition, the method is simple in process, economical, practical, low in environment pollution and significant to biotransfomation based steroid medicine production.