Method for optimization of androstenedione fermentation process

A fermentation process and androstenedione technology is applied in the field of optimizing androstenedione fermentation process, which can solve the problems of difficult androstenedione fermentation production, low utilization rate of sterol, and high cost, and achieves reduction in the rate of bacterial contamination in fermentation and the reduction of raw materials. The effect of stable supply and increased production

Inactive Publication Date: 2015-12-23
SHANDONG FANGMING PHARMACEUTICAL CO LTD
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0003] However, there are still some difficulties in the fermentation and production of androstenedione at present, such as low sterol utilization rate, large foam and high cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0023] The present invention will be further described below by shake flask and 1 ton tank experiment example, and following example is illustrative, not delimitative, can not limit protection scope of the present invention with following example.

[0024] 1. Shake flask test:

[0025] Inoculate the mature slant into the first-grade seed bottle, the filling volume is 100ml / 500ml, the temperature is 30±1°C, the rotation speed is 200r / min, the humidity is 30%-60%, and the culture is 72h. The bacteria are large and the pH value reaches about 8.0. Fermentation bottle, inoculum volume 10%.

[0026] The culture conditions of the fermentation bottle are: filling volume 30ml / 250ml, rotation speed 220r / min, culture for 7-8 days, culture temperature 30±1℃, humidity 30%-60%. Glucose and glycerin solutions were added during the fermentation process, and the feeding process was as follows: 4ml of 25% ammonium sulfate solution was added to 50ml of 32% glycerin solution, mixed evenly, and a...

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Abstract

The invention discloses a method for optimization of an androstenedione fermentation process, and is characterized in that a fermentation formula comprises the components (g / L): 25 of glucose, 27 of a soybean cake powder, 1.0 of urea, 4 of citric acid, 3 of potassium dihydrogen phosphate, 0.5 of magnesium sulfate, 0.05 of ferrous sulfate, 6 of calcium carbonate, 30 of phytosterol, 7 of Tween, 15 of soybean oil, and 2 of polyoxypropylene-polyoxyethylene glycerol ether, wherein the pH value is 8.0. The method effectively increases the solubility of sterol, improves the utilization rate of sterol, reduces bubbles generated in the fermentation process, improves the yield, reduces the fermentation bacterial-infection rate, and reduces the production cost.

Description

technical field [0001] The invention relates to a method for optimizing the fermentation process of androstenedione, belonging to the technical field of fermentation process optimization. Background technique [0002] Androstenedione is a steroid compound extracted from the testis or urine and has the effect of androgen. The pure production of androstenedione by chemical methods is far from meeting the market demand. Phytosterols can be used as intermediates of steroid hormone drugs, and can be used as raw materials for the semi-synthesis of steroid hormone drugs, and the current market demand is relatively large [1] . However, phytosterol side chains are difficult to degrade by chemical means, and have been treated as waste for a long time. However, through the selective degradation of sterol side chains by microorganisms, the important intermediate androstenedione (androstenedione), which is an important intermediate for the preparation of steroid hormone drugs, can be ob...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/02
Inventor 李瑾于新令余洪智
Owner SHANDONG FANGMING PHARMACEUTICAL CO LTD
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