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Method for detecting five androgens in serum

An androgen and serum technology, applied in the field of medical analysis and detection, can solve the problems of long derivatization time, many side reactions and high cost, and achieve the effects of shortened detection time, low detection limit and easy operation.

Inactive Publication Date: 2019-09-06
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Usually, derivatization is time-consuming, costly, and has many side reactions
In addition, some scholars use atmospheric pressure photoionization (APPI) ion source, but this mode generally requires the use of toluene, which is too toxic

Method used

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  • Method for detecting five androgens in serum
  • Method for detecting five androgens in serum
  • Method for detecting five androgens in serum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] The method for detecting 5 kinds of androgens by ultra-high performance liquid chromatography-tandem mass spectrometry provided by this embodiment comprises the following steps:

[0062] 1. Materials

[0063] The experimental samples of the methodological research came from the serum samples of Wuhan Kangshengda Medical Laboratory Co., Ltd. in June 2015.

[0064] 2. Instruments and reagent consumables

[0065] Instruments: Shimadzu 8050 triple quadrupole mass spectrometer, Shimadzu 30A ultra-high performance liquid chromatography, ultrapure water instrument (Millipore ultrapure water machine), high-speed refrigerated centrifuge (GL-20M high-speed refrigerated centrifuge), vortex Mixer (SCILOGEX, USA), electronic analytical balance (FA1004, Shanghai Sunny Hengping Scientific Instrument Co., Ltd.), GM-12 water bath nitrogen blowing instrument (Beijing Chengmeng Weiye Technology Co., Ltd.), pipette gun (Dalong Xingchuang Experimental Instrument Co., Ltd., Beijing), volum...

Embodiment 2

[0094] The method for detecting five kinds of androgens by ultra-high performance liquid chromatography-tandem mass spectrometry provided in this example has the same steps as those in Example 1, the only difference being:

[0095] The mobile phase gradient elution parameters are:

[0096]

[0097] The detection of 5 kinds of androgens is completed within 6 minutes, and the measured accuracy and sensitivity are close to those of Example 1.

experiment example 1

[0113] The methodological verification experiment was carried out on the detection methods of the five kinds of androgen in Example 1.

[0114] 1. Precision experiment

[0115]Take the actual serum samples, add low, middle and high level quality control substances respectively, carry out the precision experiment, test each concentration 5 times, and calculate the average value and relative standard deviation (RSD%) of the test results. The experimental data of methodological verification are shown in Table 3-7 respectively.

[0116] Table 3 DHEA precision data experiment (unit: ng / mL)

[0117]

[0118] Table 4 Testosterone precision data experiment (unit: ng / mL)

[0119]

[0120]

[0121] Table 5 androstenedione precision data experiment (unit: ng / mL)

[0122]

[0123] Table 6 DHT precision data experiment (unit: ng / mL)

[0124]

[0125] Table 7 androsterone precision data experiment (unit: ng / mL)

[0126]

[0127] 2. Recovery rate of standard addition ...

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Abstract

The invention provides a method for detecting five androgens in serum. The method comprises the following steps of: mixing a serum sample to be detected with an internal standard solution, adopting amixed extraction solvent, obtaining detection liquid through liquid-liquid extraction pretreatment, and detecting the detection liquid by adopting an ultra-high performance liquid chromatography-tandem mass spectrum technology. The ultra-high performance liquid chromatography conditions are as follows: mobile phase A is 0.1% formic acid aqueous solution, and mobile phase B is 0.1% formic acid methanol solution. The mass spectrum conditions are as follows: a positive ion mode, namely a mass spectrum scanning mode of multi-reaction monitoring; and five androgens, which are dehydroepiandrosterone, testosterone, androstenedione, dihydrotestosterone, and androsterone. The method of the invention has the advantages that the detection time for simultaneously detecting five androgens is only fiveminutes, and the detection flux is large; the operation is simple and convenient, and the time consumption is less; and the method has the advantages of strong specificity, high sensitivity, high precision, low detection limit, wide detection range and wide practical application prospect.

Description

technical field [0001] The invention relates to the technical field of medical analysis and detection, and more specifically, relates to a method for detecting five kinds of androgens in serum. Background technique [0002] Androgens are an important class of steroid hormones. Androgens, also known as male hormones, are hormones that maintain normal sexual desire and reproductive functions. Androgen can regulate the distribution and composition percentage of human adipose tissue, and inhibit the increase or increase of body fat. Low androgen levels can lead to obesity, abdominal fat accumulation, and possibly other metabolic diseases. Therefore, the increase or decrease of androgen is closely related to growth and development and various clinical diseases, such as precocious puberty, polycystic ovary hyperprolactinemia, congenital adrenal hyperplasia, Cushing syndrome, androgen-secreting tumors and thyroid diseases Wait. [0003] Androgen can promote the growth of male or...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/045
Inventor 王献刘琪刘杨茜
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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