Treating method for androstenedione fermented wastes and application of recovered materials

A technology of androstenedione and a processing method, applied in the field of androstenedione fermentation production, can solve the problems of easily polluting the environment, occupying the space of factories and enterprises, restricting the development of enterprises, etc., achieving simple operation, reducing production costs, and increasing enterprise benefits Effect

Active Publication Date: 2017-08-18
NANYANG INST OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The emulsion after recovering methanol contains 70-80% oil and unconverted sterols to become fermentation waste. Due to the high degree of emulsification and high concentration of oil, protein and polysaccharide, this kind of waste not only easily pollutes the environment during processing, Moreover, it also oc

Method used

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  • Treating method for androstenedione fermented wastes and application of recovered materials
  • Treating method for androstenedione fermented wastes and application of recovered materials
  • Treating method for androstenedione fermented wastes and application of recovered materials

Examples

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Effect test

Embodiment 1

[0033] The processing method of androstenedione fermentation waste, the steps are as follows:

[0034] 1) Add ethyl acetate solvent to the androstenedione fermentation waste according to the solid-liquid ratio of 1:2, and stir for 5 minutes in a water bath at 50°C, then let it stand for 2 hours, and take the ethyl acetate phase of the upper layer for use; After adding an equal volume of ethyl acetate solvent to the phase, let it stand at 50°C for 30 minutes, take the upper phase of ethyl acetate for use, and at the same time filter the lower phase to obtain a filtrate, and mix it with the above-mentioned ethyl acetate phase to obtain a mixed solution. The filter residue is discarded;

[0035] 2) The mixed solution was crystallized at 10°C and 0°C for 12 hours successively; after each crystallization, the crystals were filtered and washed with ethyl acetate solvent to obtain sterol crystals with a purity of 95%; the obtained filtrate was concentrated and degreased Melt, that i...

Embodiment 2

[0037] The processing method of androstenedione fermentation waste, the steps are as follows:

[0038] 1) Add ethyl acetate solvent to the androstenedione fermentation waste according to the solid-liquid ratio of 1:2, and stir for 5 minutes in a water bath at 60°C, then let it stand for 2 hours, and take the ethyl acetate phase of the upper layer for use; After adding an equal volume of ethyl acetate solvent to the phase, let it stand at 60°C for 30 minutes, take the upper phase of ethyl acetate for use, and at the same time filter the lower phase to obtain a filtrate, and mix it with the above ethyl acetate to obtain a mixed solution. The filter residue is discarded;

[0039] 2) The mixed solution was crystallized at 10°C and 0°C for 12 hours successively; after each crystallization, the crystals were filtered and washed with ethyl acetate solvent to obtain sterol crystals with a purity of 95%; the obtained filtrate was concentrated and degreased Melt, that is, fat.

Embodiment 3

[0041] The processing method of androstenedione fermentation waste, the steps are as follows:

[0042] 1) Add ethyl acetate solvent to the androstenedione fermentation waste according to the solid-liquid ratio of 1:3, and stir for 5 minutes in a water bath at 50°C, then let it stand for 2 hours, and take the ethyl acetate phase of the upper layer for use; After adding an equal volume of ethyl acetate solvent to the phase, let it stand at 60°C for 30 minutes, take the upper phase of ethyl acetate for use, and at the same time filter the lower phase to obtain a filtrate, and mix it with the above ethyl acetate to obtain a mixed solution. The filter residue is discarded;

[0043] 2) The mixed solution was crystallized at 10°C and 0°C for 12 hours successively; after each crystallization, the crystals were filtered and washed with ethyl acetate solvent to obtain sterol crystals with a purity of 95%; the obtained filtrate was concentrated and degreased Melt, that is, fat.

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Abstract

The invention belongs to the technical field of androstenedione production and specifically relates to a treating method for androstenedione fermented wastes and application of recovered materials. The method comprises the following steps: adding a demulsifying agent into the androstenedione fermented wastes at material liquor ratio of 1:(1-3); standing by, splitting phases and taking an upper phase under the condition at 20-60 DEG C; adding isometric demulsifying agent into a lower phase, and then standing by, splitting phases and taking the upper phase under the same temperature again; filtering the lower phase, thereby acquiring a filtrate; mixing the filtrate with the upper phase, thereby acquiring a mixed solution; crystallizing the mixed solution under the condition at 0-10 DEG C for two times, filtering and taking crystal, thereby acquiring sterol crystal; concentrating the filtrate and then precipitating the grease, thereby acquiring the grease. An organic solvent demulsification method and a low-temperature secondary crystallization technique are adopted by the invention for effectively recycling and reusing grease and sterol from the androstenedione fermented wastes, so that the production cost is saved and the pollution of the wastes is reduced.

Description

technical field [0001] The invention belongs to the technical field of androstenedione fermentation production, and specifically relates to a treatment method for androstenedione fermentation waste and an application of recyclates. Background technique [0002] The vast majority of steroid hormone drugs are produced and processed with androstenedione as raw material, because androstenedione is an indispensable intermediate in steroid hormone drugs, and has a particularly obvious regulatory effect on the body . The production of androstenedione in modern industry is mainly based on animal and plant sterols which are abundant in nature, and is produced by microbial transformation technology. [0003] With the development of industrial technology, my country has gradually adopted industrialized biotransformation methods to produce androstenedione, that is, using mainly phytosterols, supplemented by agar, glucose and other commonly used chemical products for general fermentatio...

Claims

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Application Information

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IPC IPC(8): C07J9/00C12P33/00C11B13/00
CPCC07J9/00C11B13/00C12P33/00Y02W30/74
Inventor 王莹娄源民仝飞飞刘凤霞薛刚项东方
Owner NANYANG INST OF TECH
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