Method for producing 11alpha-hydroxy-4-ene-3,17-androstanedione

Abstract

The present invention provides a method for producing 11alpha-hydroxy-4-ene-3,17-androstanedione. The method comprises use of fungi to convert substrate 4-ene-3,17-androstenedione to the 11alpha hydroxy-4-ene-3,17-androstenedione, and includes the following steps: 1) collecting spores of a desired specie, and performing seed culture; 2) placing the seeds obtained in the step 1) in a conversion tank, and using a conversion culture medium to ferment and culture; 3) crushing the substrate, and beating with a 30 to 60% (v / v) aqueous ethanol solution to form a paste; 4) continuously adding the substrate of the step 3) in batches into the conversion culture medium in the conversion tank of the step 2) for 2 to 5 times, wherein in each time of adding, the amount of the added substrate is 1-3% (w / v) of the volume of the fermented liquid in the conversion tank, and the time for each time of adding is 4 to 5 hours. Compared with the prior art, the method provided by the invention improves the feeding amount of the substrate, the utilization rate and the conversion time greatly shorten the production cycle, and the method is an efficient and environmentally friendly production method.

Description

technical field [0001] The invention belongs to the technical field of microbial transformation, and relates to a method for transforming steroidal drug intermediates, more specifically to converting 4-ene-3,17-androstadione into 11α-hydroxyl-4-ene by microbial fermentation - 3,17-androstanedione method. Background technique [0002] Microorganisms can selectively modify or transform the molecular structure of steroids, which is catalyzed by enzymes produced by microorganisms. In 1952, biochemist D.H. Peterson and microbiologist H.C. Murray of Upjohn Pharmaceuticals in the United States discovered that Rhizopus aureus can hydroxylate the 11-carbon position of progesterone to generate 11α-hydroxyprogesterone, and scientists successively discovered Some species of bacteria, fungi, and actinomycetes can change the molecular structure of steroid compounds with a certain structure at a certain position. This enzymatic reaction has strict substrate specificity, and generally one...

AI Technical Summary

Problems solved by technology

Although the microbial fermentation method is beneficial to environmental protection, it has the disadvantages of low feed amount and low yield, and due to insufficient control and optimization of fermentation in the actual production process, the cost of the product may be greatly increased, and thus reduce the company's competitiveness

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