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Herba rabdosiae extract having antibacterial activity as well as preparation method and application of herba rabdosiae extract

A technology of the extract and antibacterial activity of Rubescens japonicus, applied in the direction of organic active ingredients, medical preparations containing active ingredients, applications, etc., can solve the problems of affecting the immune effect of humans and animals, cross-infection of pathogenic bacteria, etc., and achieve prevention Effects of Feed Bacterial Contamination, Plant Resource Promotion, and Prevention of Bacterial Infection

Inactive Publication Date: 2017-05-10
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Affect the immune effect of humans and animals; (3), prone to cross-infection of pathogenic bacteria

Method used

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  • Herba rabdosiae extract having antibacterial activity as well as preparation method and application of herba rabdosiae extract

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preparation example Construction

[0022] The preparation method of the described Cordyceps japonicus extract with antibacterial activity comprises the following steps:

[0023] (1) Select the aboveground part of Dongling grass, put it in an oven to dry, separate the stems and leaves, crush the selected leaves through a traditional Chinese medicine crusher, pass through a 60-mesh sieve and put them in a vacuum glass dryer to obtain dried Dongling Grass powder, spare;

[0024] (2) Weigh the dried oriole powder prepared in the above steps, add 6-10 times the mass fraction of 95% alcohol aqueous solution, soak for 3-5 days, and stir 3-4 times a day to obtain soaked winter balsam. The mixture of lingonberry powder: add the soaked lingonberry powder mixture into the reflux device, put the reflux device into the ultrasonic generator for ultrasonic ethanol reflux extraction, the extraction temperature is 40°C, the extraction time is 30 min each time, and the ultrasonic power is 150 W , extract 2 times, combine the fi...

Embodiment 1

[0031] A kind of Oryx japonicus extract with antibacterial activity is obtained by drying, pulverizing, ultrasonically assisted ethanol reflux extraction, vacuum concentration, and vacuum freeze-drying by using Oryx japonicus as raw material. The preparation method is as follows:

[0032] (1) Select the leaves of Rubescens japonicus, which are collected from Jiyuan City, Henan Province. After drying, they are pulverized by a traditional Chinese medicine grinder, passed through a 60-mesh sieve, and placed in a desiccator for later use.

[0033] (2) Weigh 20g of the above-mentioned Rubescens japonicus, add 95% alcohol that is 6 times the mass of Rubescens japonicus, soak for 3 days, and stir 4 times a day. Put the reflux device into the ultrasonic generator for ultrasonic ethanol reflux extraction. The extraction temperature was 40°C, each extraction time was 30 min, and the ultrasonic power was 150 W. Extraction was performed twice, and the filtrates were combined.

[0034] (3)...

Embodiment 2

[0038] A kind of Oryx japonicus extract with antibacterial activity is obtained by drying, pulverizing, ultrasonically assisted ethanol reflux extraction, vacuum concentration, and vacuum freeze-drying by using Oryx japonicus as raw material. The preparation method is as follows:

[0039] (1) Select the leaves of Rubescens japonicus, which are collected from Jiyuan City, Henan Province. After drying, they are pulverized by a traditional Chinese medicine grinder, passed through a 60-mesh sieve, and placed in a desiccator for later use.

[0040] (2) Weigh 20g of the above-mentioned Rubescens japonicus, add 95% alcohol 8 times the mass of Rubescens japonicus, soak for 5 days, and stir 3 times a day. Put the reflux device into the ultrasonic generator for ultrasonic ethanol reflux extraction. The extraction temperature was 40°C, each extraction time was 30 min, and the ultrasonic power was 150 W. Extraction was performed twice, and the filtrates were combined.

[0041] (3) The filtr...

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Abstract

The invention relates to a herba rabdosiae extract having an antibacterial activity as well as a preparation method and an application of the herba rabdosiae extract. The herba rabdosiae extract consists of the following ingredients: oridonin, 5,6,7-trimethoxy flavone, 2-methoxy-4-vinylphenol, eugenol, [alpha]-linolenic acid and androstenedione. The preparation method of the herba rabdosiae extract comprises the following steps: selecting and drying herba rabdosiae, crushing the dried herba rabdosiae by virtue of a traditional Chinese medicine pulverizer, and soaking the crushed herba rabdosiae in an alcohol water solution; conducting ultrasonic ethanol reflux extraction on a soaked herba rabdosiae powder mixture so as to obtain a filtrate; and decompressing and filtering the filtrate, and concentrating the processed filtrate by virtue of a rotary evaporator at 45 DEG C so as to obtain an extractum. The herba rabdosiae extract which has the antibacterial activity is obtained by drying the extractum in a vacuum environment in a freeze-drying mode. The preparation method provided by the invention is reasonable in process; the prepared herba rabdosiae extract has a quite strong inhibitory effect on a plurality of microorganisms; and the herba rabdosiae extract, as an antibacterial active ingredient of a feed additive, can be used for preventing bacterial pollution of feed.

Description

technical field [0001] The present invention relates to a kind of Radix Ornidis extract, in particular to an extract of Radix Orbidis with antibacterial activity and its preparation method and application. Background technique [0002] Since the discovery that antibiotics can promote animal growth, antibiotic feed additives have been widely used and made great contributions to animal husbandry. However, with the abuse of antibiotics, antibiotic feed additives have three side effects: (1), causing pathogenic bacteria to develop drug resistance; (2), remaining in the body. Affect the immune effect of humans and animals; (3), prone to cross-infection of pathogenic bacteria. Scientists have confirmed that many human diseases are related to the residues of antibiotics, hormones and drugs in livestock and poultry products. With the improvement of people's living standards and health awareness, green livestock and poultry products are more and more popular among the people. The ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/53A61K31/566A61P31/04A23K10/30A61K31/352A61K31/085A61K31/202
CPCA61K31/085A61K31/202A61K31/352A61K31/566A61K36/53A61K2236/15A61K2236/333A61K2236/39A61K2236/51A61K2236/53A61K2300/00Y02A50/30
Inventor 汪伦记许美秋纠敏原江锋赵亚美
Owner HENAN UNIV OF SCI & TECH
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