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Qualitative and quantitative detection method of steroid hormones in saliva

A technology for steroid hormones and detection methods, applied in the detection field of 8 steroid hormones in human saliva, can solve the problems of poor patient compliance, inconvenient multi-point monitoring, etc. The effect of analysis

Inactive Publication Date: 2019-05-31
成都益康谱科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, fasting blood sampling is not convenient for multi-point monitoring, and patient compliance is poor

Method used

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  • Qualitative and quantitative detection method of steroid hormones in saliva
  • Qualitative and quantitative detection method of steroid hormones in saliva
  • Qualitative and quantitative detection method of steroid hormones in saliva

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. Materials.

[0039] The samples of the methodological research experiment came from the saliva samples of some patients in the Department of Endocrinology, Chengdu Women and Children's Central Hospital, Sichuan Province, from February to March 2019.

[0040] Instruments: API 4000 triple quadrupole mass spectrometer (Applied Biosystem); Agilent liquid chromatography system (with automatic sampler); one hundred thousandth balance; chromatographic column (C18); Machine; Freeze Dryer; Solid Phase Extraction Column; Adjustable Pipette; Glass Instruments, Beakers, Graduated Cylinders, etc.

[0041]Reagent consumables: chromatography pure methanol; acetonitrile; formic acid; ammonium acetate; ascorbic acid; sodium acetate; sodium bicarbonate; sodium hydroxide; acetone; acetylhydrazide trimethylammonium chloride; glacial acetic acid; sodium hydroxide; activated carbon; phosphoric acid.

[0042] Standards: DHEA, 17-hydroxyprogesterone, progesterone, androstenedione, testoste...

Embodiment 2

[0091] The samples of the research experiment came from the saliva samples of some volunteers from the Affiliated Hospital of West China School of Public Health in Sichuan Province from May to June 2019.

[0092] Among them, sample numbers 1-6; 7-9 are male samples.

[0093] Reagents and equipment are the same as in Example 1 in this experiment.

[0094] Chromatographic conditions and mass spectrometry detection conditions are the same as in Example 1 in this experiment.

[0095] The total ion diagram of the saliva sample is detected. At low concentrations, there is basically no interference from other peaks. See the attached figure 2 .

[0096] Measurement results and data statistics:

[0097]

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Abstract

The invention provides a qualitative and quantitative detection method of steroid hormones in saliva and particularly relates to a liquid chromatography tandem mass spectrometry detection method of eight steroid hormones in saliva; the steroid hormones include 17-hydroxyprogesterone, progesterone, androstenedione, testosterone, dehydroepiandrosterone, cortisol, corticosterone and aldosterone. Thedetection method herein includes: freezing a human saliva sample, allowing protein precipitation, ultrasonically extracting, filtering, mixing the filtrate with an internal standard liquid, carrying out reversed-phase SPE (solid phase extraction) column purification, and chemically deriving the target products. Full dissolution of the target hormones in saliva is guaranteed; disturbance of impurities in the saliva is eliminated; accuracy and stability are ensured for detection results. The eight hormones are synchronously, qualitatively and quantitatively analyzed by liquid chromatographic separation and mass spectrometric ion-pairing detection. The detection method herein is suitable for synchronous batch detection of hormone molecules having similar structure and approximate chemi-physical properties, has the advantages of high sensitivity, high precision, good repeatability, high detection speed and the like, fully meets the needs of clinical detection, and has high clinical application value.

Description

technical field [0001] The invention relates to the technical field of saliva sample detection, in particular to a method for detecting 8 steroid hormones in human saliva. Background technique [0002] Steroid hormones in the human body are divided into two categories according to their pharmacological effects: adrenal corticosteroid hormones and sex hormones. Steroid hormones are all synthesized from cholesterol hormones as the parent, and are a class of fat-soluble hormones. They are all cyclopentane polyhydrophenanthrene derivatives in structure. Although the functions of these hormones are quite different, they are structurally similar. Because they are all derived from cholesterol, and can be transformed into each other under the action of corresponding enzymes in the body to achieve a balanced state of steroid hormone metabolic pathways in the body. Cholesterol is first metabolized into pregnenolone, and then under the action of enzymes, part of it is converted into ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/89
Inventor 邬智刚朱文漓叶尚宇
Owner 成都益康谱科技有限公司
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