Qualitative and quantitative detection method of steroid hormones in saliva

Abstract

The invention provides a qualitative and quantitative detection method of steroid hormones in saliva and particularly relates to a liquid chromatography tandem mass spectrometry detection method of eight steroid hormones in saliva; the steroid hormones include 17-hydroxyprogesterone, progesterone, androstenedione, testosterone, dehydroepiandrosterone, cortisol, corticosterone and aldosterone. Thedetection method herein includes: freezing a human saliva sample, allowing protein precipitation, ultrasonically extracting, filtering, mixing the filtrate with an internal standard liquid, carrying out reversed-phase SPE (solid phase extraction) column purification, and chemically deriving the target products. Full dissolution of the target hormones in saliva is guaranteed; disturbance of impurities in the saliva is eliminated; accuracy and stability are ensured for detection results. The eight hormones are synchronously, qualitatively and quantitatively analyzed by liquid chromatographic separation and mass spectrometric ion-pairing detection. The detection method herein is suitable for synchronous batch detection of hormone molecules having similar structure and approximate chemi-physical properties, has the advantages of high sensitivity, high precision, good repeatability, high detection speed and the like, fully meets the needs of clinical detection, and has high clinical application value.

Description

technical field

[0001] The invention relates to the technical field of saliva sample detection, in particular to a method for detecting 8 steroid hormones in human saliva. Background technique

[0002] Steroid hormones in the human body are divided into two categories according to their pharmacological effects: adrenal corticosteroid hormones and sex hormones. Steroid hormones are all synthesized from cholesterol hormones as the parent, and are a class of fat-soluble hormones. They are all cyclopentane polyhydrophenanthrene derivatives in structure. Although the functions of these hormones are quite different, they are structurally similar. Because they are all derived from cholesterol, and can be transformed into each other under the action of corresponding enzymes in the body to achieve a balanced state of steroid hormone metabolic pathways in the body. Cholesterol is first metabolized into pregnenolone, and then under the action of enzymes, part of it is converted into ...

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