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Liquid preparation capable of degrading petroleum pollutants

A technology for liquid preparations and pollutants, which is applied in the field of liquid preparations for degrading petroleum pollutants. It can solve problems such as affecting efficiency, difficulty in degradation, and difficulty in uniform distribution of bacteria, so as to achieve the effect of improving degradation ability

Inactive Publication Date: 2016-03-09
叶君芝
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bacteria are prokaryotes, and they mainly use mono / dioxygenase intracellular oxidation system and a series of dehydrogenase systems in the biodegradation process of organic pollutants. The reaction needs to occur after the bacteria contact with petroleum hydrocarbons. Substrate utilization by bacteria is limited by low bioavailability of petroleum hydrocarbons
In addition, the most suitable substrate range of petroleum hydrocarbons and mono / dioxygenase system is C4-C20 straight-chain alkanes, so it is difficult for bacteria to degrade petroleum hydrocarbons with higher carbon number and more complex structure
Bacteria are strongly adsorbed by soil particles in the soil, which makes it difficult for the added bacteria to be evenly distributed in the contaminated area, and also affects the efficiency of bacterial biological agents used in the soil bioremediation process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1: A liquid microbial preparation was composed of a culture of the fungus C. chinensis strain 1 and the bacterium Pseudomonas 1 strain; the concentration of aloin in the liquid preparation was 0.4 mg / ml.

[0016] The liquid formulation used in this example was prepared as follows:

[0017] A 300ml Erlenmeyer flask with a volume of 100ml liquid potato medium was inoculated with C. chinensis, cultured at 28°C and 160rpm for 3 days, and the culture was collected. The fungal content was 30mg dry weight / ml. Pseudomonas was inoculated in a 300ml Erlenmeyer flask with a capacity of 100ml LB liquid medium, cultivated for 1 day at 30°C and 160rpm, and the culture was collected. The bacterial content was 10 8 pieces / ml. The obtained fungal culture and bacterial culture were mixed according to a mixing ratio of 5:1. Add aloin so that the final concentration is 0.4 mg / ml.

Embodiment 2

[0018] Example 2: A liquid microbial preparation was composed of cultures of a fungal white-rot fungus Phanerochaete chrysosporium strain 1 and a bacterium Rhodococcus strain 1; the concentration of aloin in the liquid preparation was 0.4 mg / ml.

[0019] The liquid formulation used in this example was prepared as follows:

[0020] Inoculate the white rot fungus Phanerochaete chrysosporium in a 300ml Erlenmeyer flask with a volume of 100ml liquid potato medium, cultivate it at 28°C and 160rpm for 3 days, collect the culture, and the fungal content is 30mg dry weight / ml. Inoculate Rhodococcus in a 300ml Erlenmeyer flask containing 100ml LB liquid medium, cultivate for 1 day at 30°C and 160rpm, collect the culture, and the bacterial content is 10 8 pieces / ml. The obtained fungal culture and bacterial culture were mixed according to a mixing ratio of 5:1. Add aloin so that the final concentration is 0.4 mg / ml.

Embodiment 3

[0021] Example 3: Use the cultures of 1 strain of silvery fungus C. chinensis, 1 strain of white-rot fungus Phanerochaete chrysosporium and 1 strain of bacteria Pseudomonas to form a liquid microbial preparation; the concentration of aloin in the liquid preparation 0.4mg / ml.

[0022] The liquid formulation used in this example was prepared as follows:

[0023] Inoculate Yinhamecus californica and white rot fungus Phanerochaete chrysosporium in a 300ml Erlenmeyer flask with 100ml liquid potato culture medium, culture at 28°C and 160rpm for 3 days, collect the culture, and the fungal content It is 80mg dry weight / ml. Pseudomonas was inoculated in a 300ml Erlenmeyer flask with a capacity of 100ml LB liquid medium, cultivated for 1 day at 30°C and 160rpm, and the culture was collected. The bacterial content was 10 8 pieces / ml. The obtained fungal culture and bacterial culture were mixed according to a mixing ratio of 1:1. Add aloin so that the final concentration is 0.4 mg / ml....

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PUM

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Abstract

The invention discloses a liquid preparation capable of degrading petroleum pollutants. The liquid preparation consists of a fungus liquid cultural substance, a bacterial liquid cultural substance and a degradation accelerant, wherein the mixing ratio of the fungus liquid cultural substance and the bacterial liquid cultural substance is 1: (0.1 to 10); the fungus liquid cultural substance is a single plant liquid cultural substance of cunninghamella echinulata or white-rot fungus phanerochaete chrysosporium, or a mixture of the two fungus liquid cultural substances, the content of fungi in the fungus cultural substances is 10 to 80mg dry weight / ml; the bacterial liquid cultural substance is a liquid cultural substance of a bacterial strain with the capability of petroleum hydrocarbon degradation, and the bacterial content is 106 to 1012 pieces / ml; the degradation accelerant is barbaloin, and the concentration in the liquid preparation is 0.2 to 0.6mg / ml. The degradation accelerant provided by the invention can obviously improve the degradation ability of fungi and bacteria to the petroleum pollutants.

Description

technical field [0001] The invention relates to the field of environmental protection, in particular to a liquid preparation for degrading petroleum pollutants. Background technique [0002] Bioremediation technology is the main remediation technology for soil contaminated by petroleum and related products. Due to the complex composition, biodegradability and low bioavailability of petroleum pollutants, it is difficult for indigenous microorganisms to effectively, rapidly and completely degrade petroleum hydrocarbons in soil. The biological addition method can improve the efficiency of bioremediation and effectively remove petroleum hydrocarbon pollutants by adding microbial agents with high-efficiency degradation of petroleum hydrocarbons. Therefore, strengthening the breeding of dominant bacteria and developing environmental microbial preparations are the core of oil-contaminated soil bioremediation technology. There are a variety of microorganisms that can degrade petro...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14B09C1/10C12R1/645C12R1/38C12R1/365C12R1/265C12R1/01C12R1/32C12R1/15C12R1/07
CPCC12N1/20B09C1/10B09C1/105C12N1/14
Inventor 叶君芝
Owner 叶君芝
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