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Exract of sea fungus culture and its preparation method and use

A technology of marine fungi and extracts, applied in the fields of botanical equipment and methods, fungi, applications, etc., can solve problems such as drug resistance

Inactive Publication Date: 2005-06-15
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Long-term use of these agents has resulted in parasite resistance to the agents

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Extract of the present invention—the separation and preparation method of brown oil:

[0021] a. Seed culture of fungus NO1893

[0022] Culture medium (by weight): glucose 1, yeast extract 0.1, peptone 0.2, agar 1, sodium chloride 3, water 100; make a test tube slant, pick strains and insert them into the slant, and culture at 35°C for 7 days;

[0023] b. Fermentation of fungus NO1893

[0024] Fermentation medium (by weight): 2kg of glucose, 0.2kg of yeast extract, 0.4kg of peptone, 6kg of sodium chloride, and 200L of water; pick the strains cultivated in the inclined plane into the fermentation medium, and let them stand at room temperature 25-35°C. Set 1.5 months;

[0025] c. filtering the above-mentioned cultivated fermented liquid to remove the thallus;

[0026] d. Heating and concentrating the fermented liquid to 50L of the volume of the stock solution, extracting it several times with an equal amount of ethyl acetate, concentrating the ethyl acetate e...

Embodiment 2

[0028] Example 2 The extract of the present invention-brown oily matter kills Chinese bream and cotton bollworm activity test

[0029] 1. Anti-cotton bollworm test

[0030] Dilute the brown oil obtained in Example 1 with a serial concentration of 1000 μg / ml, then evenly spray it on the leaves of Chinese cabbage, and after drying naturally, the 2nd to 3rd instar cotton bollworms artificially cultured are connected to each leaf with a brush. On the treated leaves, cultivate under the condition of 26°C, investigate the death percentage of each treatment cotton bollworm once every 24 hours, use sterile water as blank control, and each process has 3 repetitions. Result: 1000μg / ml, 24hr, 100% kill cotton bollworm.

[0031] 2. Anti-fish parasite test of Chinese bream

[0032] Use a 500ml Erlenmeyer flask to dilute the brown oil obtained in Example 1 with a series concentration, then connect the Chinese gnat to each treated Erlenmeyer flask, culture it at 26°C, and investigate the d...

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PUM

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Abstract

The present invention relates to a kind of ethyl acetate extract effective part oil of the culture endophytic fungus of South China Sea mangrove and its preparation method and this extract is used for preparing and killing cotton bollworm [Heliothis armigera (Hühner)] and fish parasite Pesticide application of Sinergasilus spp.

Description

technical field [0001] The invention relates to an extract of a South China Sea mangrove endophytic fungus culture and a preparation method thereof, as well as the application of the extract in preparing pesticides against cotton bollworm and fish parasites, Chinese gnats. Background technique [0002] Cotton bollworm Heliothis armigera (Hühner) is one of the main pests of cotton in bud stage. Cotton bollworm occurs early, damages for a long time, has a large amount of occurrence, a wide range of occurrence, unbalanced occurrence, and overlapping generations. Strong adaptability is the internal cause of the heavy occurrence of cotton bollworm in the 1990s. Cotton bollworm has a mixed diet. In addition to damaging cotton, it can also eat corn, wheat, soybeans, peanuts, fruit trees, vegetables and other plants. It is large and has a wide range of adaptability to temperature, so that the cotton bollworm can make full use of various plant reso...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/00A01N63/04C12N1/14
Inventor 林永成陈光英佘志刚李厚金周世宁林壁润关利平
Owner SUN YAT SEN UNIV
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