Method for observing and identifying filamentous fungi

Abstract

Disclosed is a method for observing and identifying filamentous fungi. The method includes the steps of adding a potato dextrose agar (PDA) culture medium into a culture dish first, cutting the PDA culture medium by using an operating knife along the diameter of the culture medium and perpendicular to the bottom of the culture dish after the PDA culture medium condenses, removing half of the culture medium, selecting to-be-identified fungus culture and inoculating the to-be-identified fungus culture to the middle of the connection portion of the culture medium and the bottom of the culture dish, covering the culture dish with a culture dish lid, carrying out marking after sealing the culture dish through a parafilm, and culturing the culture medium in dark at the temperature of 28 DEG C. After culturing is finished, the fungi grow into half of a normal bacterial colony in half of the culture dish with the culture medium, and bacterial colony characteristics can be observed; and hyphae grow sparsely in the other half of the culture dish without the culture medium, and the hyphae can be micro-observed when the culture dish is inversely placed. The method for observing and identifying the filamentous fungi has the advantages that flaking is not needed, fungus individual form observation and colony form observation are combined, operation is simple, convenience and quickness are achieved, the hyphae and the bacterial colony, in a naturally-growing state, of strain can be observed in real time at the same time, accuracy of identifying work for the filamentous fungi can be guaranteed, cost is saved, and workload is reduced.

Description

technical field [0001] The invention belongs to the field of mycological research, and in particular relates to a method for combining the observation of filamentous fungus individual morphology and colony morphology. Background technique [0002] For a long time, the research and identification of filamentous fungi has been mainly based on the combination of macroscopic feature observation and molecular identification. For macroscopic feature identification methods, the identification of filamentous fungi is mainly based on morphological features, including individual morphology and group morphology. Therefore, it is very important to obtain better hyphae microscopic characteristics and colony characteristics at the same time for fungal research and identification. However, in the traditional method of studying the morphological characteristics of filamentous fungi, the individual morphology observation and the group morphology observation are carried out separately. metho...

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Problems solved by technology

However, in the traditional method of studying the morphological characteristics of filamentous fungi, the individual morphology observation and the group morphology observation are carried out separately. method, agar tank culture method, instrument and equipment automatic film production method, etc., there are following deficiencies in the above methods: (1) mycelium picking and film production method often occurs that mycelium overlaps, and the natural growth state of mycelia is destroyed. Normal microscopic morphology of fungi cannot be obtained; (2) the slide culture method needs to make a wet chamber, the operation process is cumbersome and complicated, and the requirements for operators and experimental equipment are high; (3) the automatic production method of instruments and equipment requires the samples Under the influence of the selective filter membrane, it is limited to granular materials such as cells, bacteria, pollen, etc., and it is impossible to make slices of filamentous materials, and there are external forces in the process, so the natural state of the materials cannot be maintained. At the same time, the equipment is relatively expensive.

(4) The above methods cannot obtain the colony characteristics while obtaining the microscopic characteristics of the fungi, which increases the workload and increases the cost in the observation and identification of filamentous fungi

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