Extract of sea fungal culture and preparation method and application thereof

A technology of marine fungi and cultures, applied in the field of biomedicine, to achieve remarkable effects

Inactive Publication Date: 2009-03-18
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the phenomenon of bacterial drug resistance has become a serious problem faced by the medical circles of various countries. Now t

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Preparation of the extract of the marine fungus ZH116 culture

[0026] a. Seed culture of fungus ZH116

[0027] Medium (parts by weight): glucose 1, yeast extract 0.1, peptone 0.2, agar 1, sodium chloride 3, water 100;

[0028] The above-mentioned culture medium was made into a test tube slant, the strains were picked and inserted into the slant, and cultured at 30°C for 7 days;

[0029] b. Fermentation of fungus ZH116

[0030] Fermentation medium: weigh 1g of glucose, 0.1g of yeast extract, 0.2g of peptone and 3g of coarse sea salt, add 1L of water and mix well;

[0031] Pick the strains cultivated in the above slant into the fermentation medium, and culture them statically at room temperature 25°C for 1 month

[0032] c. filtering the above-mentioned cultivated fermented liquid to remove the thallus;

[0033] d. Extract the above-mentioned filtered fermentation broth with ethyl acetate several times, collect the upper layer extract, concentrate and evapo...

Embodiment 2

[0034] Example 2 Antibacterial, enzyme-inhibiting and antitumor activity tests of the extract

[0035] 1. Antibacterial test

[0036] The extract obtained in Example 1 was dissolved in dimethyl sulfoxide DMSO to 1000 μg / mL and then serially diluted. In a 96-well plate, 190 μl of bacterial suspension (OD600 was 0.6) was added to each well, and then the aforementioned different concentrations were added. 10 μl of the extract solution was repeated three times, 10 μL DMSO was added to the blank control, and ampicillin sodium solution was used as the positive control. After culturing at 37°C for 16 hours, the OD600 was measured. At the same time also visually.

[0037] The minimum inhibitory concentration is the concentration at which there is little growth of bacteria.

[0038] The minimum inhibitory concentrations of the extracts of the fungal ZH116 culture to 6 different pathogenic bacteria were all less than 1 μg / mL.

[0039] 2. Acetylcholinesterase inhibitory activity

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PUM

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Abstract

The invention discloses an extract for marine fungi culture, a preparation method thereof and application thereof. The extract is an oily matter prepared by the following steps: after seed culture and fermentation culture of marine fungi CCTCC M 208095, collection of culture fermentation broth, extraction of the fermentation broth by ethyl acetate and concentration and drying of the residual matter. The extract has the advantages of resisting pathogenic bacteria and oral cancer cells KB and inhibiting acetylcholinesterase, topoisomerase I, and a drug-resistant strain KBv200 of the extract, and can be used for preparing antibacterial and antineoplastic medicines for treating senile dementia.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to an extract of a marine fungal culture and a preparation method and application thereof. Background technique [0002] It is one of the main tasks for human beings to overcome diseases to find substances with strong physiological activity and develop them into anticancer, antibacterial, and Alzheimer's drugs. The study of marine microbial metabolites is an emerging research topic in the world in recent years. As an ecosystem growing in the intertidal zone of tropical and subtropical bays and estuaries, mangroves have always been in the intertidal environment of high salinity, frequent tides, strong winds, high temperatures, strong ultraviolet radiation and anoxic sludge. Their habitats are very special. This has also resulted in the unique metabolic pathway and genetic background of endophytic fungi in mangroves. Studies have found that many of their components al...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P1/02A61K36/06A61P31/00A61P25/28A61P35/00C12R1/645
Inventor 蔡小玲佘志刚林永成李庆高俊平
Owner SUN YAT SEN UNIV
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