Method for improving content of salidroside and tyrosol in rhodiola rosea tissue culture seedling

A technology of salidroside and rhodiola, which is applied in the field of plant tissue culture, can solve problems such as artificial cultivation of rhodiola, and achieve the effects of low cost, increased content, and simple operation

Inactive Publication Date: 2014-12-17
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, relying on traditional methods cannot solve the ...

Method used

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  • Method for improving content of salidroside and tyrosol in rhodiola rosea tissue culture seedling

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) The fungus ZPRa-R-1 is activated and cultivated to a vigorous state, and 5 bacterium cakes with a diameter of 0.5cm are inserted into a 500mL Erlenmeyer flask equipped with 200mL PDA liquid medium, at 28°C, the rotation speed is Under the condition of 200r / min, culture in dark for 5 days, filter with four layers of gauze, separate the mycelium and fermentation broth, discard the mycelia, and rotate the fermentation broth to form a paste for later use.

[0023] According to the ratio of 2g: 1L = fermentation broth paste: MS medium (containing 0.5mg / L TDZ, 0.5mg / L NAA), add the fungal fermentation broth paste to MS medium to make fungus and Rhodiola rosea The symbiotic medium of tissue culture seedlings was sterilized at 121°C, and after cooling, healthy tissue culture seedlings of Rhodiola grandiflora were inoculated.

[0024] (2) Blank control: no fungal culture was added, and MS medium (containing 0.5mg / L TDZ, 0.5mg / L NAA) was used to directly culture Rhodiola gran...

Embodiment 2

[0028] The solid culture bacteria are directly symbiotic with the tissue culture seedlings, and the contents of salidroside and tyrosol in the rhodiola rosea tissue culture seedlings are increased.

[0029] (1) The fungus ZPRa-R-1 is activated and cultured to a vigorous state, ready for use.

[0030] (2) Use sterilized and cooled MS medium (containing 0.5mg / L TDZ, 0.5mg / L NAA) to inoculate healthy Rhodiola rosea tissue culture seedlings. Under sterile conditions, the vigorous fungus ZPRa-R-1 was inoculated on the medium close to the tissue culture seedlings, so that the fungus and Rhodiola rosea tissue culture seedlings could grow symbiotically, and the culture conditions were set according to two periods: ① Light for 14 hours hours, the temperature is 27°C, and the humidity is 72%; ②10 hours without light, the temperature is 22°C, and the humidity is 78%. The culture time was 20 days, the seedlings were harvested, dried, crushed, extracted, and measured according to the meth...

Embodiment 3

[0032] The fungus ZPRa-R-1 was activated and cultivated to a vigorous state, and 6 pieces of bacterium cakes with a diameter of 1.0cm were inserted into a 500mL Erlenmeyer flask containing 150mL of PDA liquid medium. Under dark cultivation for 8 days, filter with four layers of gauze, separate the mycelia from the fermentation broth, discard the mycelia, and rotate the fermentation broth to form a paste for later use.

[0033] According to the ratio of 4g: 1L = fermentation broth paste: MS medium (containing 1mg / L TDZ, 1mg / L NAA), add the fungal paste to MS medium to make fungal culture and Rhodiola grandiflora seedlings The symbiotic medium was sterilized at 121°C, and after cooling, the tissue-cultured seedlings of Rhodiola grandiflora were inoculated.

[0034] The culture conditions were set according to two periods: ① 16 hours of light, temperature 28°C, humidity 75%; ② non-light 8 hours, temperature 23°C, humidity 80%. The culture time was 30 days, the seedlings were har...

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Abstract

The invention provides a method for improving content of salidroside and tyrosol in a rhodiola rosea tissue culture seedling and particularly relates to a method for improving content of salidroside and tyrosol by means of co-culturing phialocephala fortinii with the preservation number of CGMCC No. 9347 and the rhodiola rosea tissue culture seedling. The method comprises the following steps: fungal culture and culture preparation; and co-culture of the fungal culture and the rhodiola rosea tissue culture seedling, wherein the culture conditions are as follows: illumination for 12-16 hours, temperature at 25-28 DEG C and humidity at 70-75%; non-illumination for 12-8 hours, temperature at 18-23 DEG C and humidity at 75-80%; and alternate culture for 10-30 days to obtain the rhodiola rosea tissue culture seedling with high content of salidroside and tyrosol. The highest content of salidroside can reach 2.56mg/g and the highest content of tyrosol can reach 5.48mg/g. The method is simple to operate and low in cost, and is an effective method for improving the content of salidroside and tyrosol in the rhodiola rosea tissue culture seedling under a low altitude condition.

Description

technical field [0001] The invention relates to plant tissue culture, in particular to a method for increasing salidroside and tyrosol contents in rhodiola tissue culture seedlings by co-cultivating fungi and rhodiola rosea tissue culture seedlings. Background technique [0002] Rhodiola is a perennial succulent herb or subshrub belonging to Crassulaceae and Rhodiola sp. It generally grows in alpine areas above 2000 meters above sea level, and is a traditional medicinal plant that has been used in my country for nearly a thousand years. Rhodiola rosea (R.crenulata) is the original plant of medicinal rhodiola recorded in the Pharmacopoeia of my country in 2010. Heartache, stroke hemiplegia, burnout and asthma.” At present, rhodiola is mainly used for clinical treatment of brain hypoxia and other aspects; it is also widely used as an additive in health care products and cosmetics, and the demand is increasing year by year. [0003] The main chemical constituents of Rhodiola r...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N5/04C12R1/645
Inventor 崔晋龙王梦亮
Owner SHANXI UNIV
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