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Method for preparing gardenia blue pigment

A technology of gardenia blue pigment and gardenia fruit, applied in the field of preparation of high-purity gardenia blue pigment, can solve the problems of late gardenia blue pigment, low reaction rate, high cost, etc., achieve bright color, simple and easy operation, fully responsive effect

Inactive Publication Date: 2011-04-20
LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Domestic research on gardenia blue pigment started relatively late, and the method of directly adding gardenia fruit powder to the culture medium was mostly used to produce natural pigments. The color of the obtained products was mostly gray and purple, and the yield was low, so it was difficult to industrialize production
Later, some researchers improved the method and adopted a two-step method (Sun Lijun et al., using a two-step method to produce natural gardenia blue pigment, Journal of Nanjing Agricultural University, 1994, 17(4): 98-101), first using β-glucoside Enzyme catalyzes the reaction of geniposide to obtain the intermediate product genipin, and then reacts genipin and amino acids under appropriate conditions to obtain natural gardenia blue. The obtained gardenia blue color is ideal, but its reaction rate is low and the cost is high, which affects the Industrialized production (CN101104745A a kind of production method of natural blue pigment)

Method used

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  • Method for preparing gardenia blue pigment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Add 4800mL of 70% ethanol (mass ratio of 1:12) to 400g of crushed gardenia husk, extract 45min with ultrasonic circulation, extract 2 times (ultrasonic 4s, gap 1s, power 1000W, rotating speed 1100rpm), combine the 2 extractions After the solvent was recovered under reduced pressure, it was weighed and dried to obtain 40 g of gardenia husk extract.

[0034] Get gardenia fruit shell extract 5g, dissolve with the water of 50mL, join in the HPD100A and LX-10G mixed macroporous adsorption resin column by 50g (composition ratio is 1: 1, mix homogeneously), wash with 15% ethanol Remove and collect the eluate to obtain high-purity iridoid glycosides.

[0035] After concentrating the high-purity iridoid glycosides under reduced pressure without any alcoholic taste, add 0.5g glutamic acid, then add cellulase solution (pH 4, mass ratio 0.05:100), react at 60°C for 12 hours, add Dehydrated alcohol (accounting for 10% of total volume), filters, and the filtrate obtains gardenia blu...

Embodiment 2

[0037] Add 3200 mL of 70% ethanol (mass ratio of 1:8) to 400 g of pulverized gardenia husks, and the ultrasonic circulation extraction method is the same as in Example 1 to obtain 35 g of gardenia husk extract.

[0038] Get gardenia fruit shell extract 5g, dissolve with the water of 25mL, join in the HPD200A and LX-10G mixed macroporous adsorption resin column by 50g (composition ratio is 1: 0.5, mix homogeneously), wash with 45% ethanol Remove and collect the eluate to obtain high-purity iridoid glycosides.

[0039] Concentrate the high-purity iridoid glycosides to dryness under reduced pressure, dissolve them in deionized water, add 2.5g of glycine, then add cellulase solution (pH 5, mass ratio 0.1:100), and react at 35°C for 24h Afterwards, add 95% ethanol (accounting for 15% of total volume), filter, the gardenia blue powder obtained after the filtrate is concentrated and dried under reduced pressure, color value is 4.5.

Embodiment 3

[0041] Add 4800 mL of 50% ethanol (mass ratio of 1:12) to 400 g of pulverized gardenia husks, and the ultrasonic circulation extraction method is the same as in Example 1 to obtain 45 g of gardenia husk extract.

[0042]Get gardenia fruit shell extract 5g, dissolve with the water of 45mL, join in the HPD700 and LX-68 mixed macroporous adsorption resin column by 50g (composition ratio is 0.5: 1, mix homogeneously), wash with 20% ethanol Remove, collect the eluent, concentrate the high-purity iridoid glycosides under reduced pressure until there is no alcohol smell, add 1.5g lysine, and then add cellulase solution (pH 6, mass ratio is 0.3: 100), in After reacting at 50°C for 18 hours, add 70% ethanol (20% of the total volume), filter, and concentrate and dry the filtrate under reduced pressure to obtain gardenia blue powder. is 4.7.

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Abstract

The invention discloses a preparation method for extracting and preparing high-purity gardenia blue pigment from gardenia nutshells. The method comprises the following steps that: the gardenia nutshells are extracted to obtain gardenia nutshell extractum, and the extractum is mixed with macroporous absorption resin to be further eluted and purified to obtain an iridoid glycoside compound; after the eluent is concentrated, amino acid and cellulose enzyme liquid are added for direct reacting; after the reaction is finished, ethanol is added in the reacting liquid for separating and drying; and the quick preparation of high-purity gardenia blue is realized.

Description

technical field [0001] The invention relates to a preparation method of high-purity gardenia blue pigment, specifically, the invention relates to a preparation method of extracting and preparing high-purity gardenia blue pigment from gardenia husk. Background technique [0002] Gardenia is the fruit of Gardenia jasminoides Ellis (Gardenia jasminoides Ellis), which is mainly produced in the southern and central southern regions of my country. It belongs to the first batch of medicinal and edible plant resources promulgated by the Ministry of Health. There are detailed records in Li Shizhen's "Compendium of Materia Medica" in the Ming Dynasty: Gardenia has the effects of protecting the liver, promoting gallbladder, lowering blood pressure, calming, hemostasis, and reducing swelling; Blood pressure, diabetes etc. Gardenia mainly contains crocetin, crocetin and its derivatives, and 11 kinds of iridoid glycosides mainly composed of geniposide, and also contains chlorogenic acid,...

Claims

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Application Information

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IPC IPC(8): C12P1/00
Inventor 师彦平戚欢阳
Owner LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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