Method for improving active ingredients of honeysuckle flowers

An active ingredient, the technology of honeysuckle, which is applied in the field of improving the active ingredient of honeysuckle through photoenzyme induction technology, can solve the problems of unfriendly light pollution, low application value, and weak penetration of ultraviolet light, etc., and achieve short ultraviolet irradiation time and high efficiency , suitable for large-scale industrial production

Active Publication Date: 2018-01-23
山东省医学科学院
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For such an irradiation time, the actual application value is often very low for industrial production, because the first industrial production is often ton-level production, and ultraviolet treatment for 4 or 6 hours requires extremely high cost and energy consumption, and there are Light pollution is not environmentally friendly; second, due to t

Method used

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  • Method for improving active ingredients of honeysuckle flowers
  • Method for improving active ingredients of honeysuckle flowers
  • Method for improving active ingredients of honeysuckle flowers

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0038] Construction of Fingerprint of Total Extract of Lonicerae japonica

[0039] (1) Freshly isolated honeysuckle flower buds are dried and pulverized, and passed through a 40-mesh medicine sieve. Weigh 1.0g, weigh it accurately, wrap it with filter paper and put it in a jar, add 200ml of methanol and place it in an ultrasonic generator for ultrasonic extraction for 60min (the temperature is controlled at about 30°C), pour out the supernatant, and add 100ml of methanol Continue to extract until colorless, combine the supernatants obtained twice, evaporate to dryness (60° C.) with a rotary evaporator, and dilute the residue into a 10 ml volumetric flask with methanol. After shaking well, take 1.5 mL, centrifuge at room temperature 10000 rpm for 10 minutes, pass the supernatant through a 0.45 μm filter membrane, analyze by HPLC, and finally optimize the fingerprint spectrum of the total extract of Clematis paniculata by changing the mobile phase. The chromatographic condition...

experiment example 2

[0047] Changes of fingerprints under different induction conditions:

[0048] After the induction was completed according to the conditions listed in Table 1, both the examples and the comparative examples were cultured under suitable conditions (temperature at 25° C. and humidity at 60%) completely protected from light. After 24 hours of cultivation, the honeysuckle was dried in an oven (60° C.), crushed, and passed through a 40-mesh medicine sieve. According to the method for establishing the fingerprints of the total extract of Flos Lonicerae Flos Lonicerae described in Experimental Example 1 above, the fingerprints of the examples and comparative examples were established and compared and analyzed. The results are shown in Table 3 and Table 4.

[0049] It can be known from the results that the content of caffeoylquinic acid compounds and iridoids in Lonicerae japonica active ingredients changed the most under the conditions of freezing for 1 hour, C0.5A0.5B0.5 ultraviolet ...

experiment example 3

[0057] Small scale industrial experiment:

[0058] (1) Get 200kg of fresh isolated honeysuckle;

[0059] (2) Put 200kg of honeysuckle into -20°C freezer for 1 hour;

[0060] (3) After the freezing is completed, put the honeysuckle layered into the ultraviolet radiation cabinet, and under the condition of 28°C, C0.5A0.5B0.5 ultraviolet radiation is irradiated alternately;

[0061] (4) After irradiating, take a sample, according to the method established by the total extract fingerprint of Flos Lonicerae Flos Lonicerae described in the above experimental example 1, set up the fingerprint, and compare and analyze, the results are shown in the experimental example 3 in table 3 and table 4.

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Abstract

The invention relates to a method for improving the active ingredients of honeysuckle flowers, in particular to a method for improving the active ingredients of honeysuckle flower by a photoenzymaticinduction technology. The method comprises the following sequential steps of (1) taking the honeysuckle flowers; (2) performing -20 DEG C freezing; (3) performing alternate illumination by UV-A being320 to 400nm, UV-B with the wave length being 280 to 320nm and UV-C with the wave length being 200 to 280nm. The method has the advantages that the contents of caffeoyl quinic acid and iridoid glycoside of the active ingredients of the honeysuckle flowers can be obviously increased; the ultraviolet treatment time is greatly shortened; the environment-friendly effect is achieved; meanwhile, small experiments prove that the method is applicable to large-scale industrial production.

Description

technical field [0001] The present invention relates to a method for increasing the active ingredient of honeysuckle, more specifically relates to a method for increasing the active ingredient of honeysuckle through the technology of photoenzyme induction. Background technique [0002] Lonicera japonica Thunb is the dry flower bud or the first flower of Lonicera japonica Thunb. It has been known as a good medicine for clearing away heat and detoxification since ancient times, and is one of the commonly used clinical medicines. Honeysuckle is sweet and cold in nature, fragrant and fragrant. It is sweet and cold to clear away heat without hurting the stomach. , sore throat, rash, spotting, toxic heat and bloody dysentery, sore throat, febrile disease fever embolism, all effective, the effect is remarkable. In recent years, studies have found that honeysuckle is rich in a variety of biologically active substances that are beneficial to human health, such as volatile oils, flav...

Claims

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Application Information

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IPC IPC(8): A61K36/355A61P39/06A61K133/00
Inventor 马鲁豫潘晴孙京海
Owner 山东省医学科学院
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