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Preparation method for honeysuckle extract

A technology of honeysuckle extract and medicinal materials, which is applied in the field of preparation of honeysuckle extract, can solve the problems of inability to comprehensively understand the medicinal effect and detection of honeysuckle extract, and achieve the effect of overall transfer, low preparation cost, and controllable conditions

Active Publication Date: 2014-10-01
LUNAN PHARMA GROUP CORPORATION
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese patent CN101530449 discloses a method for preparing honeysuckle extract by using membrane filtration technology, including decocting and extracting honeysuckle with water, concentrating and centrifuging under reduced pressure, removing most impurities with microfiltration membrane, and further refining with ultrafiltration membrane , the transfer rate of chlorogenic acid in the obtained extract reaches 90%, but this method uses microfiltration membrane and ultrafiltration membrane to separate and purify, and does not detect the medicinal ingredients other than chlorogenic acid, and removes impurities while removing Other medicinal ingredients
Although chlorogenic acid, luteolin, total organic acids or total iridoid glycosides are one of the medicinal components of honeysuckle, only a single component is used as an indicator, and the medicinal effect of honeysuckle extract cannot be fully understood

Method used

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  • Preparation method for honeysuckle extract
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  • Preparation method for honeysuckle extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Anti-influenza A H1N1 Influenza Virus Test of Honeysuckle Extract

[0029] 1 Test purpose

[0030] To investigate the pharmacodynamics of honeysuckle extract by using influenza A H1N1 influenza virus infection mouse model of pneumonia.

[0031] 2 Test materials

[0032] 2.1 Test drugs

[0033] Honeysuckle extract, batch number: 20111927 (the honeysuckle extract obtained in Example 13 of the present invention), brown-black powder, bitter smell, crisp texture, soluble in water and ethanol.

[0034] 2.2 Positive control drug Yinhuang Oral Liquid: produced by Lunan Houpu Pharmaceutical Co., Ltd.; national drug approval: Z10870001; production batch number: 09122001; production date: 091203; valid until 201211; properties: reddish-brown clear liquid, sweet, Slightly bitter; functions and indications: clearing heat and dispelling wind, relieving sore throat and detoxifying. It is used for dry throat, sore throat, swollen laryngeal nucleus, thirst, and fever cause...

Embodiment 2

[0053] Example 2 Establishment of the Fingerprint of Honeysuckle Extract

[0054] 1 Instruments and reagents

[0055] Agilent1100 high-performance liquid chromatography (Agilent Technology Co., Ltd.), AG285 analytical balance (Mettler Toledo); pHS-25 acidity meter (Shanghai Weiye Instrument Factory); glass chromatography column (1cm × 50cm).

[0056] Honeysuckle, purchased from Jiujianpeng Honeysuckle Professional Cooperative; Luteoloside (luteoloside, purity ≥ 98%, batch number 111720-201307, China Institute for Food and Drug Control); Chlorogenic acid (3-O-CQA, purity ≥ 98%, Batch number 110753-200413, China Institute for Food and Drug Control); neochlorogenic acid, cryptochlorogenic acid (5-O-CQA, 4-O-CQA, purity ≥ 98%, batch numbers are MUST-10091501, MUST-10091811, Chengdu Master Biological Technology Co., Ltd.); 4,5-O-dicaffeoylquinic acid, 3,5-O-dicaffeoylquinic acid, 3,4-O-dicaffeoylquinic acid (4 ,5-DCQA, 3,5-DCQA, 3,4-DCQA, purity ≥ 98%, batch numbers are 12022804,...

Embodiment 3

[0067] Embodiment 3 Macroporous resin purification process research

[0068] 2.4 Purification of macroporous resin

[0069] 2.4.1 Resin pretreatment Soak the resin in absolute ethanol for 24 hours, put it into a glass chromatography column, wash it with absolute ethanol at a flow rate of 2BV / h until the effluent is not turbid after adding deionized water, and then wash it with deionized water Rinse until there is no alcohol smell, then rinse with 2BV5% dilute hydrochloric acid solution at a flow rate of 2BV / h, then rinse the resin with deionized water until the pH of the effluent is neutral, and finally use 2BV2% sodium hydroxide solution at a flow rate of 2BV / h After rinsing, rinse the resin with deionized water until the pH value of the effluent is neutral, filter with suction, and remove the water for later use.

[0070] 2.4.2 Resin Screening Accurately weigh 5.0g of the pretreated resin into a stoppered Erlenmeyer flask, accurately add 40mL of honeysuckle extract, place i...

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Abstract

The invention relates to a preparation method for a honeysuckle extract. The method comprises the steps as follows: firstly, ethanol extracting; secondly, vacuum concentrating; thirdly, pH value regulating; fourthly, macroporous resin purifying; fifthly, concentrating, drying and the like. The technology of the method is simple and easy to operate; as the macroporous resin can be recycled, the method is low in manufacturing cost and small in environmental pollution; the ethanol solution, adopted as an extracting and eluting solvent, is high in safety, cheap and easy to obtain, and low in production cost; as the obtained honeysuckle extract and the honeysuckle have basically identical ingredients, namely compounds such as an organic acid, iridoid glycoside, and flavone, an active substance group is wholly transferred; under the situation that invalid substances are removed effectively, the recycling rate of the organic acid, the iridoid glycoside, and the flavone can reach higher than 80%, and the resemblance of the extract finger-print before and after the macroporous resin purification can reach over 0.91.

Description

technical field [0001] The invention relates to a preparation method of honeysuckle extract, which belongs to the field of traditional Chinese medicine. Background technique [0002] "Multi-component, multi-target, overall effect" is the characteristic of traditional Chinese medicine. Its efficacy depends on the synergistic effect of a series of compound groups with relatively stable composition. In the process, the recovery rate of each component varies greatly, which destroys its inherent composition and will inevitably affect its function. For example, macroporous resin adsorption technology is currently considered to be the most promising technology for purification of traditional Chinese medicine, and its application research in the field of traditional Chinese medicine is increasing. However, one or several components are used as indicators to optimize the purification conditions. Active ingredients with different properties have different retention rates. After resin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/355A61P31/16A61P31/04A61P29/00
Inventor 赵志全李守信李蔚群
Owner LUNAN PHARMA GROUP CORPORATION
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