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Method for simultaneously separating quercetin-3-O-gentian diglucoside and kaempferol-3-O-gentian diglucoside from folium sauropi

A technology for the simultaneous separation of gentiobiosides, applied in chemical instruments and methods, sugar derivatives, sugar derivatives, etc., can solve the problems of low quercetin and kaempferol content, complicated process, and low purity, and achieve Low dissolution rate, easy operation and high separation efficiency

Active Publication Date: 2017-05-17
CHENGDU PUSH BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the process of this technical solution is complicated, the cost is high, and there are too many types of solvents involved, and the content of quercetin and kaempferol in the extracted flavonoids is low, and the purity is not high; and the quercetin and chrysin involved , apigenin, kaempferol and luteolin are flavone aglycones, but no flavone glycosides are mentioned. Because the polarity of flavone aglycones and flavone glycosides is very different, this technical solution is not suitable for Extraction and separation of quercetin-3-0-gentiobioside and kaempferol-3-0-gentiobioside

Method used

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  • Method for simultaneously separating quercetin-3-O-gentian diglucoside and kaempferol-3-O-gentian diglucoside from folium sauropi
  • Method for simultaneously separating quercetin-3-O-gentian diglucoside and kaempferol-3-O-gentian diglucoside from folium sauropi
  • Method for simultaneously separating quercetin-3-O-gentian diglucoside and kaempferol-3-O-gentian diglucoside from folium sauropi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Such as figure 1 , figure 2 Shown: The method for simultaneously separating quercetin-3-0-gentiobioside and kaempferol-3-0-gentiobioside from gragon leaves includes the following process steps:

[0044] (1) Extract

[0045] Weigh 1Kg of the medicinal material of serrata leaf and pulverize it into 1~4mm coarse powder, then add 6L of 70% ethanol solution, reflux and extract 3 times, 2 hours / time, filter, and combine the filtrate, a total of 30L, which is the extract I;

[0046] (2) Concentration

[0047] After recovering ethanol from the extract I obtained in step (1), it is concentrated to a small volume, and the concentrated liquid obtained is 5L, which is the extract II;

[0048] (3) Enrichment and elution of macroporous resin column

[0049] The extract II obtained in step (2) is adsorbed with a macroporous resin, the resin is washed with 10L purified water until it is colorless, and then the product section is eluted with 15L of 60% ethanol solution until the effluent is col...

Embodiment 2

[0065] The method for simultaneously separating quercetin-3-0-gentiobioside and kaempferol-3-0-gentiobioside from the leaves of Glycine max includes the following process steps:

[0066] (1) Extract

[0067] Weigh 10Kg of the medicinal material of serrata leaf, pulverize it into a coarse powder of 1~4mm, add 60L of 70% ethanol solution, extract 3 times, 3 hours / time, filter, combine the filtrate, a total of 90L, it is the extract I;

[0068] (2) Concentration

[0069] After recovering ethanol from the extract I obtained in step (1), it is concentrated to a small volume, and the concentrated liquid obtained is 15 L, which is the extract II;

[0070] (3) Enrichment and analysis of macroporous resin

[0071] The extract II obtained in step (2) is adsorbed with a macroporous resin, and the resin is washed with 80L purified water until it is colorless, then 120L of 65% ethanol solution is used to elute the product section until the effluent is colorless, collect and combine Eluent: Concentra...

Embodiment 3

[0087] The method for simultaneously separating quercetin-3-0-gentiobioside and kaempferol-3-0-gentiobioside from the leaves of Glycine max includes the following process steps:

[0088] (1) Extract

[0089] Weigh 20Kg of the medicinal material of serrata leaf, pulverize it into a coarse powder of 1~4mm, then add 120L of 70% ethanol solution, extract 4 times, 3 hours / time, filter, combine the filtrate, a total of 185L, which is the extract I;

[0090] (2) Concentration

[0091] After recovering ethanol from the extract I obtained in step (1), it is concentrated to a small volume, and the concentrated liquid obtained is 30L, which is the extract II;

[0092] (3) Enrichment and analysis of macroporous resin

[0093] The extract II obtained in step (2) is adsorbed with a macroporous resin, and the resin is washed with 150L of purified water until it is colorless, and then 200L of 70% ethanol solution is used to elute the product section until the effluent is colorless. Collect and combine ...

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Abstract

The invention relates to a method for simultaneously separating quercetin-3-O-gentian diglucoside and kaempferol-3-O-gentian diglucoside from folium sauropi and belongs to the technical field of separation and purification of a compound monomer. According to the invention, the folium sauropi medicinal material is taken as a raw material, and the quercetin-3-O-gentian diglucoside and kaempferol-3-O-gentian diglucoside monomers are acquired according to the steps of ethanol extraction, concentration, macro-porous resin enrichment and elution, polyamide resin enrichment and elution, efficient preparation, liquid chromatography separation and product recycling. According to the invention, through mature processing steps and parameter conditions, the purities of the quercetin-3-O-gentian diglucoside and the kaempferol-3-O-gentian diglucoside in the product respectively reach above 99%; and meanwhile, the method is simple and convenient in operation and is high in separating efficiency, the raw materials are recyclable, and the quercetin-3-O-gentian diglucoside and kaempferol-3-O-gentian diglucoside monomers can be simultaneously separated and prepared in large batch and high purity.

Description

Technical field [0001] The present invention relates to a method for separating and purifying plant compounds, in particular to a method for simultaneously separating quercetin-3-0-gentiobioside and kaempferol-3-0-gentiobioside from Gentiana leaves, belonging to Chinese medicine Technical field. Background technique [0002] Dragon Leaf Sauropus rostratus It is a plant of the genus Euphorbiaceae, also known as Agave Leaf, Dragon Leaf Leaf, Dragon Leaf Leaf, and Niu'er Leaf. It is mainly distributed in Guangdong, Guangxi, Fujian and other places. It is native to northern Vietnam and cultivated in the Malay Peninsula. It has the effects of clearing heat and moisturizing the lungs, resolving phlegm and relieving cough. It is used to treat diseases such as lung heat, cough, excessive phlegm, dry mouth and constipation. The current reports on Longliye are limited to some preliminary discussions, and there are few studies on its chemical components and pharmacological activities. The...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08
CPCC07H1/08C07H17/07Y02P20/582
Inventor 陈冲李波徐仕银夏柯刘丁
Owner CHENGDU PUSH BIOLOGICAL TECH
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