A kind of preparation method and application of quercetin-3-gentiobioside

A technology of gentiobioside and quercetin, which is applied in the preparation of sugar derivatives, preparations for skin care, chemical instruments and methods, etc., can solve problems such as undiscovered, and achieve the effect of strong antioxidant activity

Active Publication Date: 2021-08-13
YUNNAN BOTANEE BIO TECH GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Quercetin-3-gentiobioside was not found to be isolated from the plant of the genus Pachyphyllum in previous literature reports. This compound has certain antioxidant activity and can be used as an internal control substance for the quality control of the husk extract of Papaya.

Method used

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  • A kind of preparation method and application of quercetin-3-gentiobioside
  • A kind of preparation method and application of quercetin-3-gentiobioside
  • A kind of preparation method and application of quercetin-3-gentiobioside

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Effect test

Embodiment 1

[0050] A preparation method of quercetin-3-gentiobioside, comprising the following steps:

[0051] S1, crushing step: crushing 200g of dried fruit shell of Chonglou to obtain 80-mesh fine powder of Chonglou fruit;

[0052] S2, the step of reflux extraction: put the fine powder of Papaya chinensis into the extraction tank, and use 70% ethanol / water mixed solution to carry out reflux extraction for 3 times to obtain the extract, the amount of ethanol / water mixed solution is 1Kg, The temperature is 75-90°C, the time for each reflux extraction is 2 hours, and the extracts obtained by 3 reflux extractions are combined;

[0053] S3, step of concentrating under reduced pressure: concentrating the extract under reduced pressure at a temperature of 60° C., removing the alcohol in the extract to obtain 100 g of the concentrate;

[0054] S4, centrifugation step: centrifuge the concentrated solution at a speed of 3000 rpm, take out the supernatant, and redissolve the centrifuged precipit...

Embodiment 2

[0066] A preparation method of quercetin-3-gentiobioside, comprising the following steps:

[0067] S1, crushing step: crushing 205g of dried fruit shell of Chonglou to obtain 80-mesh fine powder of double fruit shell;

[0068] S2, the step of reflux extraction: put the fine powder of Pachyphylla chinensis into the extraction tank, use 1.6Kg 80% ethanol / water mixture to carry out 3 times of reflux extraction to obtain the extract, the temperature of the reflux extraction is 75-90°C, every The time of the secondary reflux extraction is 2 hours, and the extracts obtained by the 3 reflux extractions are combined;

[0069] S3, step of concentrating under reduced pressure: concentrating the extract under reduced pressure at a temperature of 60° C., removing the alcohol in the extract to obtain 120 g of the concentrate;

[0070] S4, centrifugation step: centrifuge the concentrated solution at a speed of 3000 rpm, take out the supernatant, and redissolve the centrifuged precipitate a...

Embodiment 3

[0081] A preparation method of quercetin-3-gentiobioside, comprising the following steps:

[0082] S1, pulverizing step: crushing 197g of dried fruit husk of Chonglou to obtain 80-mesh fine powder of Chonglou husk;

[0083] S2, the step of reflux extraction: put the fine powder of Papaya chinensis into the extraction tank, use 80% ethanol / water mixture to carry out 3 times of reflux extraction to obtain the extract, the amount of ethanol / water mixture is 1.6Kg, and reflux extraction The temperature is 75 ~ 90 ℃, the time of each reflux extraction is 2 hours, and the extracts obtained by 3 reflux extractions are combined;

[0084] S3, step of concentrating under reduced pressure: concentrating the extract under reduced pressure at a temperature of 60° C., removing the alcohol in the extract to obtain 101 g of the concentrate;

[0085] S4, centrifugation step: centrifuge the concentrated solution at a speed of 3000 rpm, take out the supernatant, and redissolve the centrifuged p...

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Abstract

The invention discloses a preparation method of quercetin-3-gentiobioside, which comprises a crushing step, a reflux extraction step, a decompression concentration step, a centrifugation step, a macroporous resin enrichment step, and a preparation liquid chromatography enrichment step and drying step, the quercetin-3-gentiobioside prepared by the preparation method of the present invention has stable quality and can be added to products such as skin care and beauty treatment as an antioxidant substance, and can also be used as a reference substance to treat the Extracts were quality controlled.

Description

technical field [0001] The invention relates to a preparation method and application of quercetin-3-gentiobioside. Background technique [0002] RHIZOMA PARIDIS is a traditional Chinese medicinal material, and its base source is the rhizome of the plant of the genus Paris in the Liliaceae (Lilliaceac). The aliases of Chonglou include Caoheche, Huazhonglou, Aescinus, unicorn lotus, golden clematis, and Aescinus chinensis, etc. There are 24 species of Chonglou in the world, mainly distributed in the tropical and temperate regions of Eurasia, and 19 species in my country, mainly distributed in Yunnan, Guangxi, Sichuan, Guizhou, Shaanxi and other provinces or autonomous regions. [0003] Chonglou has a long history of medicinal use. It was first included in "Shen Nong's Materia Medica", and it was recorded as a lower grade in "Compendium of Materia Medica" written by Li Shizhen under the name of Suxiu; The 2010 edition of Pharmacopoeia included Parispolyphylla Smith var.yunnan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/07C07H1/08A61P39/06A61Q19/08
CPCA61Q19/08A61P39/06C07H1/08C07H17/07
Inventor 袁永雷曲丽萍邓亚雷王飞飞马骁高绍阳郭振宇
Owner YUNNAN BOTANEE BIO TECH GRP CO LTD
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