Yeast fused strain mixed bacterial agent and method of fermenting organic waste water therewith to prepare liquid fertilizer

Abstract

The invention mainly relates to a yeast fused strain mixed bacterial agent and method of fermenting organic waste water therewith to prepare a liquid fertilizer. The yeast fused strain mixed bacterial agent is mainly composed of, by weight, 55-75% of yeast fused strain F105, 20-35% of aboriginal bacillus megaterium B-36, and 10-15% of aspergillus niger A-16, wherein a strain of the yeast fused strain is preserved in China General Microbiological Culture Collection Center at Dec.30, 2014 and is assigned the accession number of CGMCC NO.10263; the strain of the aboriginal bacillus megaterium is preserved in China General Microbiological Culture Collection Center at Dec.30, 2014 and is assigned the accession number of CGMCC NO.10264; and the aspergillus niger is a commen commercial bacterial strain. By means of combination of the yeast fused strain with the aboriginal bacillus megaterium and the aspergillus niger, the prepared yeast fused strain mixed bacterial agent can be used for preparing the liquid fertilizer by fermenting the organic waste water. The mixed bacterial agent not only can be used as a fermentation agent of bio-organic fertilizers, a plant production aid and a poultry, livestock and fish feed additive, but also can be used as a purifying agent in organic waste water treatment. The mixed bacterial agent has various functions and applications.

Description

technical field [0001] The invention mainly relates to a yeast fusion bacteria mixed agent and a method for preparing liquid fertilizer by fermenting organic waste water. Background technique [0002] Most of the microbial strains used in traditional microbial mixtures are purchased from national or local strain collection centers or directly isolated and screened from samples collected from nature. Its disadvantages are: 1. The activity of microbial strains is poor; 2. The ability to decompose organic matter is weak; 3. The number of living cells of the bacterial agents prepared by using these strains is small, and most of them are within 10 8 per milliliter (or gram). [0003] There are a variety of microbial bacterial agents in China, such as Baifeng EM, Wotu EM, and VT bacterial agents in Beijing. The first two EMs are Japanese Higa Teruo EM strains, and the latter is isolated from bacteria stored in the strain bank and natural samples. Bacteria culture, and the geneti...

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Problems solved by technology

Its disadvantages are: 1. The activity of microbial strains is poor; 2. The ability to decompose organic matter is weak; 3. The number of living cells of the bacterial agents prepared by using these strains is small, and most of them are within 10 8 pcs / ml (or gram)

[0003] There are a variety of microbial bacterial agents in China, such as Baifeng EM, Wotu EM, and VT bacterial agents in Beijing. The first two EMs are Japanese Higa Teruo EM strains, and the latter is isolated from bacteria stored in the strain bank and natural samples. Bacteria culture, and the genetically engineered bacteria (yeast fusion bacteria) prepared by protoplast fusion technology and the indigenous Bacillus megaterium, Aspergillus niger to prepare yeast fusion bacteria mixed agent is still blank

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