Rat-strain-knee-osteoarthritis model building method

A knee osteoarthritis and model building technology, applied in the field of medicine and biology, can solve problems such as the influence of repair treatment evaluation and the difficulty in controlling the degree of model damage

Inactive Publication Date: 2016-12-21
AFFILIATED HOSPITAL OF ZUNYI MEDICAL COLLEGE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these models can cause corresponding pathological changes, they are not consistent with the pathogenesis and mechanism of KOA and the clinical progression of osteoarthritis caused by overuse knee articular cartilage injury, and there are obvious problems such as difficulty in controlling the degree of damage in the model. And the evaluation of repair therapy is also greatly affected by various irreversible tissue damage factors in model preparation.

Method used

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  • Rat-strain-knee-osteoarthritis model building method
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  • Rat-strain-knee-osteoarthritis model building method

Examples

Experimental program
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Effect test

Embodiment

[0104] The present invention is described in further detail below:

example 1

[0105] Example 1: The establishment and application of a new model of rat overuse knee osteoarthritis, including the following specific processes:

[0106] 1. After the rats were weighed, they were anesthetized by intraperitoneal injection of 7% chloral hydrate at a dose of 0.5ml / 100g, and the breathing conditions of the rats were observed and recorded; A sharp knife was used to incise the skin along the medial border of the patellar ligament, with an incision of 2.0 to 3.0 cm. Then use ophthalmic scissors to open the joint capsule, and bluntly separate the fat pad tissue at the intercondylar fossa to expose the intercondylar fossa until the medial collateral ligament and anterior cruciate ligament can be observed with the naked eye; a small amount of bleeding during the operation was stopped by pressing with sterile gauze. Make the surgical field of view clearer and keep the joint cavity moist with saline-soaked gauze, dislocate the patella laterally to expand the surgical fi...

Embodiment 2

[0111] Embodiment two: the construction of experimental animal running platform device, comprises following specific process:

[0112] The main body of the experimental animal treadmill is a sliding-belt treadmill with adjustable speed and movement angle. The characteristics of the transformation on its basis are:

[0113] 1. Add a self-made soft cloth restraint belt, and use the cross method to restrain the chest, and draw on both sides so that it can stand upright on the treadmill, and the breathing movement will not be affected.

[0114] 2. Increase the horizontal support baffle, so that the forelimbs of upright rats can be supported on the horizontal bar, and there is a point of support.

[0115] 3. Place food and set up a black hole directly in front of the rat, and make use of its habit of eating and avoiding light, so that it can move forward autonomously.

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Abstract

The invention relates to a rat-strain-knee-osteoarthritis model building method. The rat-strain-knee-osteoarthritis model building method is combined with the primary-risk-factor joint acute or chronic biomechanics effect, the clinical strain-knee-osteoarthritis pathogenetic process is efficiently simulated, a novel animal model similar to the clinical knee-osteoarthritis pathogenesis is built, and in other words, experiment rats are only subjected to anterior cruciate ligament transection technology, but meniscuses of rats are not excised, and metabolic regulation and the stress effect of the rat knee joint micro-environment are highly kept; on the basis that a rat-knee-joint instable model is built, rats is in the forced upright bearing state at the same time, certain-strength running sport loads are fed, the joint stress effect is increased in a upslope-movement mode, a knee-joint-cartilage long-term strain is simulated, a knee osteoarthritis animal model is built, the model forming efficiency of the method and the rough time required by different-period molding of the knee osteoarthritis model are explored, and the basis is laid on further studying the mechanism and the treatment means of knee osteoarthritis.

Description

technical field [0001] The invention belongs to the field of medical biotechnology, relates to sports medicine, bone and joint disease and behavioral functional studies, tissue engineering and cell therapy, in particular to a method for establishing a rat strain knee osteoarthritis (knee osteoarthritis, KOA) model . technical background [0002] KOA is a very common arthritic disease in middle-aged and elderly people with complex etiology and characterized by knee articular cartilage strain and degeneration. A series of pathological changes bring continuous pain to patients. Its exact pathogenesis has not yet been explored clearly, but studies have found that its disease-related risk factors include age, acute or chronic biomechanical effects, articular cartilage or bone tumors, and metabolic disturbances in the cartilage microenvironment. The acute or chronic biomechanical changes of the joints and the stability changes of the surrounding ligaments may be the primary fact...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D1/00A61D7/00A01K67/02
CPCA61D1/00A01K67/02A61D7/00
Inventor 余丽梅罗欣张中原彭笳宸
Owner AFFILIATED HOSPITAL OF ZUNYI MEDICAL COLLEGE
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