Analysis method of cecal metabolome in broiler chicken

An analytical method and technology for broilers, applied in the field of biological analysis, can solve problems such as less research

Pending Publication Date: 2019-03-26
JILIN AGRICULTURAL UNIV
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Problems solved by technology

Using metabolomics to evaluate the effects of different diets on animal growth and metabo...

Method used

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  • Analysis method of cecal metabolome in broiler chicken
  • Analysis method of cecal metabolome in broiler chicken
  • Analysis method of cecal metabolome in broiler chicken

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Preparation of materials:

[0027] 1) Test animals and additives:

[0028] AA broilers were purchased from Jilin Dexiang Company; Saccharomyces cerevisiae cultures were provided by Jilin Agricultural University Ji Nong Borui Research and Development Center (fermentation times were 12h, 24h, 36h, 48h, 60h); Danongwei Yikang VXP (Danong Granville China Limited).

[0029] 2) Main reagents:

[0030] N,O-bis(trimethylsilane)trifluoroacetamide (BSTFA containing 1% TMCS), trimethylchlorosilane (TMCS) (US Sigma-Aldrich Company), pyridine (chromatographically pure, Shanghai Aladdin Biochemical Technology Co., Ltd. Co., Ltd.), n-hexane (US TEDIA Company), methoxyamine hydrochloride (US Sigma-Aldrich Company).

[0031] 3) Main instruments and software

[0032] Agilent7890A / 5975C gas chromatography-mass spectrometry (Agilent Corporation, USA), equipped with Agilent chemical workstation: MSD Chem station (E.02.01.1177, Agilent), automatic sampler (Agilent7683), Agilent HP-5MS (3...

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Abstract

The invention relates to the field of biological analysis methods, and in particular to an analysis method of cecal metabolome in a broiler chicken. The change of metabolites and metabolic pathways ofcecal contents after the broiler chicken eats a yeast culture produced at different fermentation time is detected by jointly using a gas chromatography-mass spectrometry, thereby determining the influence of the yeast culture on the cecal metabolic regulation and the metabolites of the broiler chicken. Metabolomics data show that yeast culture is used for feeding the broiler chicken, the cecal differential metabolites mainly involve in amino acid metabolism and cholesterol metabolism, and participates in fat, vitamin digestion and absorption processes.

Description

technical field [0001] The invention relates to the field of biological analysis methods, in particular to an analysis method for broiler caecum metabolites. Background technique [0002] Trillions of microbial communities in living organisms maintain a mutualistic symbiotic relationship with their parasitic hosts. These microorganisms have hundreds of times more genetic code than the human genome, and the sum of their genes is the "microbiome". The intestinal microflora is a microbiome rich in microbiota discovered by modern scholars. Research on the gut microbiome is already a hot field today. It is closely related to the occurrence and development of many diseases such as metabolic syndrome and obesity. The intestinal flora affects the host's health by affecting the host's metabolic and immune capabilities. Therefore, exploring the effects of microecological preparations such as yeast cultures on chicken intestinal microflora and their metabolites will further promote...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/88
CPCG01N30/02G01N30/88G01N2030/025
Inventor 孙喆甄玉国王涛张学峰赵巍陈雪赵小丽郑艳秋张玲玲张维刚谭胜男
Owner JILIN AGRICULTURAL UNIV
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