Structure and major gene locus Psr9 of pod shattering resistance character of rape and application thereof

A major gene and gene locus technology, applied in the fields of molecular biology and genetics and breeding, can solve the problems of quantitative traits being easily affected by environmental conditions, long cycle, poor selection effect, etc., achieving convenient and rapid detection and high cost. , the effect of improving the selection efficiency

Inactive Publication Date: 2011-10-05
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Quantitative traits are susceptible to environmental conditions, so selection does not work

Method used

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  • Structure and major gene locus Psr9 of pod shattering resistance character of rape and application thereof
  • Structure and major gene locus Psr9 of pod shattering resistance character of rape and application thereof
  • Structure and major gene locus Psr9 of pod shattering resistance character of rape and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A method for preparing the main gene locus Psr9 of the anti-cracking angle trait of rapeseed, the steps of which are:

[0041] a) The population used in this example is the F2 offspring of high and low cleavage-resistant parents (zy72360 with a cleavage-resistant index of 0.94 and R1 with a cleavage-resistant index of 0.06). The anti-cracking angle index of parents, F1 and F2 seeds was determined by random collision method. The results of the data distribution of the anti-crack angle index of the F2 generation segregation population showed that the performance distribution of the anti-crack angle trait was a continuous distribution, but the variation distribution did not show a normal distribution, which proved that the crack angle resistance trait was a quantitative trait and there was a main effect gene locus (see figure 1 ). The combination of zy72360 and R1 was used to cross rapeseed with high and low anti-cracking angles, and the F1 generation was self-crossed to...

Embodiment 2

[0061] The application of the main effect gene locus Psr9 of rapeseed's high cracking horn resistance trait breeding, and the P507 sequence of the A9 linkage group where it is located in the rapeseed high cracking horn trait breeding, the steps are:

[0062] a) The population used in this example is the F2 offspring of high and low cleavage-resistant parents (zy72360 with a cleavage-resistant index of 0.94 and R1 with a cleavage-resistant index of 0.06). The F2 generation materials are planted in the field, and numbered according to individual plants. In this embodiment, 400 individual plants are numbered, and the numbers are 001-400.

[0063] b) Extract DNA from 400 individual plants of the F2 generation numbered 001-400 at the seedling stage by taking a small piece of leaf. The extraction method refers to the DNA extraction method in Example 1.

[0064] c) Using the marker P507 primer of Psr9, the main gene locus of the anti-cracking angle trait of rapeseed, to perform PCR a...

Embodiment 3

[0069] A structure highly correlated with anti-splitting angle traits in rapeseed, application of fruit petal and placenta frame combined area index in breeding for anti-splitting angles. The steps are:

[0070] a) Apply in conjunction with Example 2. For the 89 F2 generation plants screened by molecular markers in Example 2, take siliques at the late stage of silique development, observe and measure the combined area index of the fruit petals and the placenta frame through a dissecting microscope .

[0071] b) The results show that among the 89 F2 generation plants screened by molecular markers, 56 (63%) plants have a combination area index of fruit petals and placenta frame ≥ 2mm 2 . The anti-cracking angle test results after the seeds were harvested showed that among the 56 plants screened by molecular markers and the combined area index of fruit petals and placenta frame, 48 (accounting for 86%) had an anti-cracking angle index above 0.80. That is to say, 86% of the obt...

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Abstract

The invention discloses a structure and a major gene locus Psr9 of a pod shattering resistance character of a rape and application thereof. A preparation method of the major gene locus Psr9 of the pod shattering resistance character of the rape comprises the following steps of: (a) hybridizing by utilizing a high-low pod shattering resistance assembly zy72360 of the rape and R1, and carrying out selfing on an F1 generation to generate an F2 generation segregation population; (b) extracting a parent zy72360 and R1, the leaf total DNA of the F1 generation and F2 generation segregation population; (c) collecting rape SSR (Simple Sequence Repeat) label public databases, autonomously developing an SSR label, and screening polymorphic primers by utilizing the parent zy72360; (d) establishing a genetic map through distributions inside the F2 generation segregation population, carrying out QTL (Quantitative Trait Loci) locus analysis by dint of pod shattering resistance index data, and obtaining the SSR label closely concatenated with a major gene of the pod shattering resistance character; and (e) observing the parent zy72360 and the structure of a mature pod of the F2 generation segregation population through a dissecting microscope, and measuring the combining area index of a carpel and a placenta frame. The invention has the advantages of enhanced selection efficiency, definite position of the pod shattering resistance major gene locus Psr9, convenience and fastness for detection, fast screening of a high pod shattering resistance strain used for the pod shattering resistance breeding of the rape, definite breeding selection target and cost saving.

Description

technical field [0001] The invention belongs to the technical fields of molecular biology and genetic breeding. More specifically, it relates to a rapeseed anti-cracking angle trait structure, a main effect gene locus, and a molecular marker closely linked to the main effect gene loci, and also relates to an identification method for rapeseed rape crack angle resistance trait structure and the main effect gene locus Psr9. It involves the application of the structure and molecular markers of anti-cracking horns in the breeding of rapeseed for cracking horns. Background technique [0002] Rapeseed is the third largest oil crop in the world and the fifth largest crop in my country after rice, wheat, corn and soybean. About 13% of the world's vegetable oil comes from rapeseed. In addition to being the main raw material for edible oil, rapeseed is also an important industrial raw material and a major biological resource for renewable energy in the main member states of the Europ...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12N15/10C12Q1/68
Inventor 王汉中华玮胡志勇刘贵华王新发黄顺谋詹高淼
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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