Glioma molecule typed gene group and application thereof

A glioma, gene technology, applied in the direction of microbial measurement/testing, biochemical equipment and methods, etc.

Active Publication Date: 2014-12-03
北京金岱生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there is still a lack of effective clinical diagnosis of glioma based on gene expression in the world.

Method used

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  • Glioma molecule typed gene group and application thereof
  • Glioma molecule typed gene group and application thereof
  • Glioma molecule typed gene group and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1. Screening of glioma typing marker gene group and determination of typing criteria

[0061] 1. Screening of glioma typing marker gene group and determination of typing criteria

[0062] Using the glioma gene expression profile database GSE4290 published by NCBI

[0063] (http: / / www.ncbi.nlm.nih.gov / geo / query / acc.cgi?acc=GSE4290&submit.x=0&submit.y=0), through Pearson correlation analysis, obtained 37 A gene cluster consisting of genes coexpressed with platelet-derived growth factor receptor A (PDGFRA) (termed PM genes) and a gene cluster consisting of genes coexpressed with platelet-derived growth factor receptor A (PDGFRA) (called PM genes). The two gene groups are independent of each other and do not overlap. Unsupervised hierarchical clustering analysis found that the expression levels of the PM gene group and the EM gene group in the glioma samples of the database showed three specific patterns: high expression of PM genes and low expression of EM genes ...

Embodiment 2

[0096] Example 2. Application of glioma typing marker gene groups and typing criteria in predicting prognosis and survival of glioma patients to be tested

[0097] 1. Detection of gene expression

[0098] 1. Case selection and specimen processing

[0099] The 209 samples came from glioma cases admitted to Beijing Tiantan Hospital from 2006 to 2009 (patients were informed; China Glioma Genome Atlas Project http: / / jzl.dajiankang.com / portal.php), based on WHO’s Glioma classification criteria, pathological types include astrocytoma (Astrocytoma grade II, 58 cases; Astrocytoma grade III, 8 cases), glioblastoma (Astrocytoma grade IV, GBM, 79 cases), oligodendroglioma Glioma (Oligodendroglioma grade II, 18 cases; Oligodendroglioma grade III, 11 cases), oligoastrocytoma (Oligoastrocytoma grade II, 20 cases; Oligoastrocytoma grade III, 15 cases).

[0100] The cancer tissue samples were confirmed by pathological diagnosis, quick-frozen in liquid nitrogen, and stored at -80°C.

[0101...

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PUM

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Abstract

Provided in the present invention is a set of gene groups for predicting or for the auxiliary prediction of the survival time prognosis of patients with neuroglioma, consisting of a PM gene group and an EM gene group with a total of 68 genes. A subtype marking gene group consisting of two major gene groups co-expressed with PDGFRA and EGFR can stably divide neuroglioma samples derived from different database sources into three specific subtypes, and can be applied to clinical diagnosis for neuroglioma and judging the survival time prognosis of patients with neuroglioma.

Description

technical field [0001] The present invention relates to the field of biotechnology, in particular to glioma molecular typing genogroups and their applications, and mainly relates to the study of tumor-related gene expression profiles in functional genomics and the screening and verification of marker gene groups, that is, in the range of human functional genomes According to the mRNA expression level of these genes, the glioma samples can be classified and diagnosed, which can predict the prognosis of patients. technical background [0002] Glioma is the most common primary tumor of the central nervous system and a major threat to human health. Most patients with high-grade glioma survive only 1-2 years. Low-grade gliomas will eventually develop into high-grade gliomas, but there are huge individual differences in the speed of progression. The essential reason for the slow progression of low-grade glioma is still unclear, and no effective therapeutic target for glioma has ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6886C12Q2600/112C12Q2600/118C12Q2600/158C12Q1/6876
Inventor 樊小龙孙颖郁江涛
Owner 北京金岱生物科技有限公司
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