Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Glioma molecule typed gene group and application thereof

A glioma, gene technology, applied in the direction of microbial measurement/testing, biochemical equipment and methods, etc.

Active Publication Date: 2014-12-03
北京金岱生物科技有限公司
View PDF0 Cites 20 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there is still a lack of effective clinical diagnosis of glioma based on gene expression in the world.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Glioma molecule typed gene group and application thereof
  • Glioma molecule typed gene group and application thereof
  • Glioma molecule typed gene group and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1. Screening of glioma typing marker gene group and determination of typing criteria

[0061] 1. Screening of glioma typing marker gene group and determination of typing criteria

[0062] Using the glioma gene expression profile database GSE4290 published by NCBI

[0063] (http: / / www.ncbi.nlm.nih.gov / geo / query / acc.cgi?acc=GSE4290&submit.x=0&submit.y=0), through Pearson correlation analysis, obtained 37 A gene cluster consisting of genes coexpressed with platelet-derived growth factor receptor A (PDGFRA) (termed PM genes) and a gene cluster consisting of genes coexpressed with platelet-derived growth factor receptor A (PDGFRA) (called PM genes). The two gene groups are independent of each other and do not overlap. Unsupervised hierarchical clustering analysis found that the expression levels of the PM gene group and the EM gene group in the glioma samples of the database showed three specific patterns: high expression of PM genes and low expression of EM genes ...

Embodiment 2

[0096] Example 2. Application of glioma typing marker gene groups and typing criteria in predicting prognosis and survival of glioma patients to be tested

[0097] 1. Detection of gene expression

[0098] 1. Case selection and specimen processing

[0099] The 209 samples came from glioma cases admitted to Beijing Tiantan Hospital from 2006 to 2009 (patients were informed; China Glioma Genome Atlas Project http: / / jzl.dajiankang.com / portal.php), based on WHO’s Glioma classification criteria, pathological types include astrocytoma (Astrocytoma grade II, 58 cases; Astrocytoma grade III, 8 cases), glioblastoma (Astrocytoma grade IV, GBM, 79 cases), oligodendroglioma Glioma (Oligodendroglioma grade II, 18 cases; Oligodendroglioma grade III, 11 cases), oligoastrocytoma (Oligoastrocytoma grade II, 20 cases; Oligoastrocytoma grade III, 15 cases).

[0100] The cancer tissue samples were confirmed by pathological diagnosis, quick-frozen in liquid nitrogen, and stored at -80°C.

[0101...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Provided in the present invention is a set of gene groups for predicting or for the auxiliary prediction of the survival time prognosis of patients with neuroglioma, consisting of a PM gene group and an EM gene group with a total of 68 genes. A subtype marking gene group consisting of two major gene groups co-expressed with PDGFRA and EGFR can stably divide neuroglioma samples derived from different database sources into three specific subtypes, and can be applied to clinical diagnosis for neuroglioma and judging the survival time prognosis of patients with neuroglioma.

Description

technical field [0001] The present invention relates to the field of biotechnology, in particular to glioma molecular typing genogroups and their applications, and mainly relates to the study of tumor-related gene expression profiles in functional genomics and the screening and verification of marker gene groups, that is, in the range of human functional genomes According to the mRNA expression level of these genes, the glioma samples can be classified and diagnosed, which can predict the prognosis of patients. technical background [0002] Glioma is the most common primary tumor of the central nervous system and a major threat to human health. Most patients with high-grade glioma survive only 1-2 years. Low-grade gliomas will eventually develop into high-grade gliomas, but there are huge individual differences in the speed of progression. The essential reason for the slow progression of low-grade glioma is still unclear, and no effective therapeutic target for glioma has ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6886C12Q2600/112C12Q2600/118C12Q2600/158C12Q1/6876
Inventor 樊小龙孙颖郁江涛
Owner 北京金岱生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com