Gene LBA5 for regulating and controlling lateral branch angle, growth habit and plant type of peanut, and application thereof

A technology of growth habit and gene, applied in the field of biotechnology improvement or genetic improvement, can solve the problems of not obtaining reports on peanut side branch angle or plant type-related genes and their applications, etc.

Active Publication Date: 2019-12-20
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Existing studies are limited to this, and there is no systematic molecular genetic research on peanut plant type represented by peanut sid...

Method used

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  • Gene LBA5 for regulating and controlling lateral branch angle, growth habit and plant type of peanut, and application thereof
  • Gene LBA5 for regulating and controlling lateral branch angle, growth habit and plant type of peanut, and application thereof
  • Gene LBA5 for regulating and controlling lateral branch angle, growth habit and plant type of peanut, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Implementation 1: Preliminary positioning of side branch angles in peanuts using BSA-seq technology

[0030] In this example, a creeping plant-type peanut variety Xiaohongmao with a base angle of 90 degrees between side branches and the main stem and an upright plant-type peanut variety Henan Nanyang Peanut were used to construct a hybrid combination, and a strain propagation method was obtained. A recombinant inbred line RIL-HN-F composed of 223 individuals 6 ; The traits of side branch angle, growth habit and plant type were investigated for this recombinant inbred line, and 30 families with larger side branch angles were compared with Xiaohongmao parents and 26 families with smaller side branch angles were compared with Henan Nanyang parents. Similar families, then form 2 DNA mixed pools P (creeping) pool and Z (upright) pool, sequenced on the machine, the sequencing depth of both parents is >20×, and the sequencing depth of the mixed pool is >30×, and compared with ...

Embodiment 2

[0033] Implementation 2: Using BSR-seq technology to locate side branch angles in peanuts

[0034] In this example, the recombinant inbred line PF-F constructed by using another erect variety Pingdu 9616 and the creeping variety Florunner 5 , respectively selected 30 creeping strains and 30 erect strains, and carried out transcriptome sequencing on two parents, 30 creeping strains and 30 erect strains respectively, and the sequencing output of each progeny was not less than 3Gb Clean Date, the sequence output of each parental sample is not less than 6Gb Clean Date, and compared with the published cultivated peanut Tifrunner genome as a reference; using the SNP data found by transcriptome sequencing, 30 individuals are regarded as a mixed pool ΔSNP-index ( figure 2 ), the peanut side branch angle, growth habit and plant type related loci were also located at the end of the B05 chromosome, which was consistent with the HN population positioning results. At the same time, acco...

Embodiment 3

[0035] Implementation 3: Gene cloning, structure and function prediction of LBA5b

[0036] (1) LBA5b gene cloning:

[0037] Extract the total RNA from the side branch stem end of the creeping plant type peanut Tifrunner, reverse transcribe it into cDNA, use the cloning primer pair B5cd-F / R (its sequence SEQ ID NO: 17 / 18) to carry out PCR amplification with the cDNA of Tifrunner as a template, and PCR The product was recovered and purified by gel, connected to the T vector, transformed into Escherichia coli for sequencing, and the strain with the correct sequence was kept for later use; the specific PCR amplification reaction was: PCR system was 25ul, of which 2×Gflex PCR Buffer (Mg2+, dNTP plus) was 12.5ul, Template 1ul, 10um upstream and downstream primers 1ul, Tks Gflex DNA Polymerase 1ul, add water to 25ul; PCR reaction program: 94°C pre-denaturation 1min; 98°C denaturation 10s, 55°C annealing 15s, 68°C extension 30s, cycle 35 times;

[0038] (2) LBA5b gene structure analy...

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Abstract

The invention belongs to the technical field of biotechnology and genetic engineering, and provides a gene LBA5 for regulating and controlling a lateral branch angle, growth habit and plant type of apeanut, and application thereof. A major gene LBA5 for controlling the lateral branch angle, growth habit and plant type of the peanut is located and cloned in the peanut, and comprises two homologousgenes LBA5b and LBA5a and promoters thereof; genetic improvement of an included angle between a lateral branch and a main stem of the peanut can be achieved through selection of gene allelic variation via hybridization, backcross and the like; the functions or expression quantity of the gene in a creeping variety peanut can be regulated so that the included angle between the lateral branch and the main stem of the peanut is regulated; and thus, the two homologous genes LBA5b and LBA5a and the promoters thereof of the gene LBA5 can be applied to the genetic improvement and genetic engineeringimprovement of the lateral branch angle, growth habit and plant type of the peanut, even other crops.

Description

Technical field: [0001] The invention belongs to the fields of plant biotechnology and plant genetic engineering, and relates to a plant type gene LBA5 for regulating the side branch angle and growth habit of peanuts and its application. The gene LBA5 and its homologous genes are used to perform biotechnology on the plant type of peanuts and other crops. Improvement or genetic improvement. Background technique: [0002] The growth angle of the side branches of the plant determines the plant shape of the whole plant. For most plants, the side branches are generally non-vertical, which is convenient for the group to obtain more resources such as sunlight and air. The genetic control of lateral branch angle has been well studied in model plants (Roychoudhry and Kepinski 2015). Early on, it has been studied to separate the gravitropic set-point angle (GSA) from the gravitropic growth zone (Digby and Firn 1995). The (dorsal) gravitropic determination of side branch angle is reg...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82C12N15/113C12N15/11A01H5/00A01H6/54
CPCC07K14/415C12N15/113C12N15/8218C12N15/8261C12N2310/20C12N15/8262A01H5/10A01H6/541
Inventor 张晓军颜凡壮于晓娜张绍静司彤邹晓霞王月福王铭伦
Owner QINGDAO AGRI UNIV
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