Molecular marker of brown planthopper-resistant major gene bph7 of rice and application thereof

A technology of anti-BPH and main gene, applied in the field of molecular genetics, can solve complex and difficult problems such as effective introduction and aggregation of insect-resistant genes, and achieve the effects of difficulty, shortening the breeding cycle, and convenient identification

Active Publication Date: 2011-09-14
WUHAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the complexity of identifying insect resistance in rice materials, it is often difficult to

Method used

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  • Molecular marker of brown planthopper-resistant major gene bph7 of rice and application thereof
  • Molecular marker of brown planthopper-resistant major gene bph7 of rice and application thereof
  • Molecular marker of brown planthopper-resistant major gene bph7 of rice and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] (1) 9311 / T12 F 2 Population construction and phenotyping

[0041] (1) In 1988, Kabir & Khush used insect resistance screening to find that the rice variety T12 was resistant to the brown planthopper population (biotype 4). Genetic analysis showed that the variety carried a pair of recessive brown planthopper resistance genes and named it for bph7 (Kabir & Khush, 1988 Genetic analysis of resistance to brown planthopper in rice, Oryza sativa L. Plant Breeding 100, 54–58). The rice variety comes from the germplasm resource bank of the Chinese Academy of Agricultural Sciences, and T12 comes from the rice germplasm resource bank of the International Rice Research Institute. Insect resistance identification experiments showed that T12 had moderate resistance to the brown planthopper population in my country. In order to find simple and efficient and compatible with bph7 Tightly linked molecular markers, in the present invention, the insect-susceptible variety 9311 i...

Embodiment 2

[0064] Example 2 Verification of molecular markers

[0065] 1. Materials and methods

[0066] 1.1 Materials

[0067] Negative varieties: 10 copies, 8 copies of non-insect-resistant materials in the breeding combination of susceptible variety 9311, Taichung Local No. 1 (TN1), and 9311 X T12.

[0068] Positive varieties: 9 insect-resistant materials from the breeding combination of insect-resistant variety T12 and 9311 X T12.

[0069] Molecular marker primers: RM28295, RM519, RM28305, RM3448, RM313, RM28374.

[0070] 1.2 Method

[0071] Genomic DNA of rice samples was extracted by CTAB extraction method (the method is the same as in Example 1). Sample DNA was amplified with primers RM28295, RM519, RM28305, RM3448, RM313 and RM28374, respectively. The reaction system included 0.10 μM primers, 250 μM dNTPs, 1× PCR reaction buffer (50 mM KCl, 10 mM Tris-HCl pH8.3, 1.5 mM MgCl 2 ), 100ng DNA template, 1U Taq enzyme. The reaction program was: 94°C pre-denaturation for 5 minu...

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Abstract

The invention provides a molecular marker of a brown planthopper-resistant major gene bph7 of rice and application thereof. The resistance gene bph7 of an insect-resistant variety T12 is obtained by performing genetic linkage analysis on genotypes of various F2 individual strains obtained by hybridizing the insect-resistant variety T12 (female parent) and an insect-susceptible variety 9311 (male parent) of the rice and brown planthopper resistance levels of various F2:3 families, and is positioned between a molecular marker RM3448 and a molecular marker RM313. Molecular markers, namely RM28295, RM519, RM28305 and RM28374 are also linked with the gene. The molecular marker can effectively detect whether the insect-resistant variety T12 and derived varieties (strains) thereof contain a siteof the major gene, so that the selection efficiency of brown planthopper-resistant rice is greatly improved, and a brown planthopper-resistant rice variety containing the bph7 gene is obtained.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and relates to rice main gene for resistance to brown planthopper bph7 The molecular marker, and the invention also relates to the application of the molecular marker in breeding rice varieties resistant to brown planthopper. Background technique [0002] Rice is one of the most important food crops in the world. At the same time, it is also attacked by different pathogens and pests, which seriously threatens rice production. Among them, the brown planthopper is one of the most serious insect pests in the rice planting process. Its adults and nymphs suck the juice in the phloem of rice with needles, which causes the leaves of the plant to turn yellow. No harvest. According to the China Agricultural Yearbook, in the six years of 1966, 1969, 1973, 1977, 1983 and 2003, the national brown planthopper had a large outbreak; . The occurrence area of ​​brown planthopper has reached more than 50% of...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 何光存邱永福杜波陈荣智祝莉莉
Owner WUHAN UNIV
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