Molecular marking method for major quantitative trait loci(QTL) for rice grain length

A rice grain and molecular marker technology, applied in the field of molecular genetics and breeding, can solve the problem that grain weight-related traits have no cytoplasmic effect, etc., and achieve the effects of quick and simple identification method, cost saving and high selection efficiency.
CN102154471AInactive Publication Date: 2011-08-17NANJING AGRICULTURAL UNIVERSITY
1 Cites 14 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
NANJING AGRICULTURAL UNIVERSITY
Publication Date
2011-08-17
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention belongs to the field of genetic breeding science and discloses a molecular marking method for a major quantitative trait loci (QTL) for rice grain length. In the method, Indel and simple sequence repeats (SSR) are used to mark one or more pairs of primers of XJ24 and XJ26 and RM15575 respectively to perform the polymerase chain reaction (PCR) amplification of rice genome DNA, PCR amplification products undergo an electrophoretic test on 8 percent non-denaturing polyacrylamide gel, and if DNA fragments in corresponding size are generated through amplification, qGL 3.2 synergisticallelic mutant genes exist. When the large-grain germplasm of rice and breeding populations constructed by various parents of rice are detected by the grain length QTL qGL3.2 coseparated and closely interlocked molecular marking method, the major gene qGL 3.2 for controlling grain length and weight can be quickly imported, the selecting efficiency of the major gene qGL 3.2 is improved, and the method contributes to appearance improvement and high-yield breeding of rice.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the field of molecular genetics and breeding, and relates to a molecular marker method for rice grain length main QTL sites, which is specially used for molecular marker-assisted improvement of rice variety grain length, molecular marker-assisted yield breeding and utilization of germplasm resources. Background technique

[0002] As the world's population grows, rice, a staple food crop, faces an urgent need to increase yields. In order to meet the needs of human beings, rice genetic breeders have conducted more and more in-depth research on rice yield and grain shape.

[0003] Yield traits are quantitative traits controlled by polygenes, which show continuous variation and are greatly affected by the environment. Among the intricate yield factors, direct constituent factors include grain weight, number of spikelets per panicle, effective panicle number and seed setting rate. In addition, plant height, tiller number, panicle ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More