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Molecular marking method for major quantitative trait loci(QTL) for rice grain length

A rice grain and molecular marker technology, applied in the field of molecular genetics and breeding, can solve the problem that grain weight-related traits have no cytoplasmic effect, etc., and achieve the effects of quick and simple identification method, cost saving and high selection efficiency.

Inactive Publication Date: 2011-08-17
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

Preliminary studies show no cytoplasmic effects for grain weight-related traits

Method used

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  • Molecular marking method for major quantitative trait loci(QTL) for rice grain length
  • Molecular marking method for major quantitative trait loci(QTL) for rice grain length
  • Molecular marking method for major quantitative trait loci(QTL) for rice grain length

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Embodiment 1

[0029] (1) Materials and methods:

[0030] 1. Materials: N411 and 93-11 were crossed with giant grain germplasm, and the hybrid was planted, and F was obtained by selfing 2 Segregated populations are used for the detection and positioning of the main effect grain length QTL.

[0031] 2. Extract individual DNA by SDS method.

[0032] 3. Screening of polymorphic markers: 976 pairs of SSR primers were used to perform PCR amplification with DNA of N411 and 93-11 as templates, and polymorphic markers were screened.

[0033] 4.PCR reaction: the volume is 25 microliters, including 2.5 microliters of 10×buffer, 25mM MgCl 21.5 microliters, 2.5 microliters of primer pair (4 pmol / microliter), 2 microliters of 2.5mM dNTPs, 0.2 microliters of Taq enzyme (5 units / microliter), 20 nanograms of template DNA, and add water to 25 microliters. The SSR reaction program was DNA pre-denaturation at 95°C for 5 min, followed by [(95°C denaturation for 30 s, 50°C annealing for 30 s, 72°C extension f...

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Abstract

The invention belongs to the field of genetic breeding science and discloses a molecular marking method for a major quantitative trait loci (QTL) for rice grain length. In the method, Indel and simple sequence repeats (SSR) are used to mark one or more pairs of primers of XJ24 and XJ26 and RM15575 respectively to perform the polymerase chain reaction (PCR) amplification of rice genome DNA, PCR amplification products undergo an electrophoretic test on 8 percent non-denaturing polyacrylamide gel, and if DNA fragments in corresponding size are generated through amplification, qGL 3.2 synergisticallelic mutant genes exist. When the large-grain germplasm of rice and breeding populations constructed by various parents of rice are detected by the grain length QTL qGL3.2 coseparated and closely interlocked molecular marking method, the major gene qGL 3.2 for controlling grain length and weight can be quickly imported, the selecting efficiency of the major gene qGL 3.2 is improved, and the method contributes to appearance improvement and high-yield breeding of rice.

Description

technical field [0001] The invention belongs to the field of molecular genetics and breeding, and relates to a molecular marker method for rice grain length main QTL sites, which is specially used for molecular marker-assisted improvement of rice variety grain length, molecular marker-assisted yield breeding and utilization of germplasm resources. Background technique [0002] As the world's population grows, rice, a staple food crop, faces an urgent need to increase yields. In order to meet the needs of human beings, rice genetic breeders have conducted more and more in-depth research on rice yield and grain shape. [0003] Yield traits are quantitative traits controlled by polygenes, which show continuous variation and are greatly affected by the environment. Among the intricate yield factors, direct constituent factors include grain weight, number of spikelets per panicle, effective panicle number and seed setting rate. In addition, plant height, tiller number, panicle ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 王建飞张晓军张红生王才林王州飞黄骥鲍永美蓝虹霞
Owner NANJING AGRICULTURAL UNIVERSITY
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