Sudden cardiac death mutant gene detection kit

Abstract

The invention relates to the field of molecular biology and medical science, and in particularly relates to a sudden cardiac death mutant gene detection kit which is high in accuracy and good in predictability. The kit is used for performing polymorphism detection on 12 SNP sites of 8 major genes related to sudden cardiac death; three forward and reverse specific primers are respectively adopted for each SNP site by combining the characteristics of specific allelic gene PCR (polymerase chain reaction) and temperature gradient descent PCR; and the mutation of the 12 SNP sites can be simultaneously determined by virtue of program amplification of the temperature gradient descent PCR and agarose gel electrophoretic analysis. The detection kit provided by the invention is strong in specificity, high in detection rate, high in efficiency and low in cost, and can be used for screening high-risk population of sudden cardiac death, evaluating the risk degree of having the sudden cardiac death for a detected patient, and making clear pathogenic factors of patient sudden death from a gene level, thereby providing a new way of preventing, diagnosing and treating clinical sudden death.

Description

technical field [0001] The invention belongs to the fields of molecular biology and medicine, and in particular relates to a sudden cardiac death mutation gene detection kit. Background technique [0002] Sudden Cardiac Death (SCD) refers to sudden death due to various cardiac causes. It can occur in patients with or without heart disease, usually without any life-threatening early manifestations, sudden loss of consciousness, and death within 1 hour after the onset of acute symptoms. It is a non-traumatic natural death, characterized by unexpected and rapid death . Sudden cardiac death is a complex disease affected by multiple factors such as environmental factors and genetic factors. At present, the trend of sudden cardiac death is becoming more and more obvious, which seriously threatens the health and family happiness of young adults and the elderly. [0003] At present, electrocardiograms and color Doppler echocardiography are mainly used to diagnose sudden death high...

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Problems solved by technology

The gene polymorphism can affect the excitability of the sympathetic nerve, affect the excitability of atrial myocytes, increas

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